United States
Germany
Japan
United Kingdom
China
Ercc3 (NM_001031644) Rat Tagged ORF Clone
CAT＃: RR215076
- TrueORF®
Ercc3 (Myc-DDK-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug)
"NM_001031644" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 10,070.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC112916 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR215076 representing NM_001031644
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAAAAGAGATCGAGTGGACCGCGACAAGAAGAAATCCAAGAAGAGGCAGTATGAAGAGGAAGAGG AAGACGAAGACGACGCTCCTGGGAACGAGTCTCAGGAAGCGGTGCCCTCAGCCGCTGGGAAACAGGTGGA CGAATCCAGCACCAAAGTGGATGAATATGGAGCAAAGGACTACAGACAGCAGATGCCACTAAAGGGTGAC CATACTTCTAGGCCCCTCTGGGTGGCTCCTGATGGCCACATTTTCTTGGAAGCCTTCTCTCCAGTTTACA AATATGCCCAAGACTTCCTGGTGGCAATTGCAGAGCCAGTGTGCCGGCCCACGCATGTACACGAATACAA GCTGACCGCCTACTCCCTCTATGCAGCTGTCAGTGTTGGGCTGCAGACCAGTGACATCACCGAGTACCTC AGAAAGCTCAGTAAGACTGGAGTTCCTGATGGAATCATCCAGTTTATTAAGCTGTGCACTGTCAGCTACG GAAAAGTCAAGCTGGTCCTGAAGCACAACAGGTACTTTGTTGAAAGTTCTCACCCTGATGTCATCCAGCA TCTTCTTCAAGACCCAGTAATACGGGAATGTCGCTTGAGGAATGCCGAGGGAGAGGCTACTGAACTCATC ACAGAGACGTTTACGAGCAAATCTGCTATTTCTAAGACTGTGGAAGGCAGTGGTGGGGCTTCTACTTCAC AGGGGGTAGATGCGCAAGCCAAATCTGACATCCCCAAAGACCTGTTTGATTTTTATGAGCAAATGGACAA GGATGAGGAGGAGGAGGAAGAGACACAGACAGTCTCCTTTGAAGTTAAGCAGGAGATGATCGAGGAGCTC CAGAAGCGCTGCATCTGCCTAGAGTACCCGCTGTTGGCAGAGTATGACTTCCGGAATGACTCTCTGAACC CCGATATCAACATTGACCTGAAGCCCACAGCCGTGCTCAGGCCCTATCAGGAGAAGAGCCTGCGCAAGAT GTTTGGCAATGGGCGAGCACGCTCAGGAGTCATTGTTCTTCCCTGTGGTGCTGGGAAGTCCCTGGTGGGC GTGACTGCCGCGTGTACTGTCAGAAAGCGCTGTCTGGTCCTGGGCAACTCGGCTGTGTCTGTGGAGCAGT GGAAAGCCCAGTTTAAGATGTGGTCAACCATCGATGACAGCCAGATCTGCCGCTTCACCTCAGATGCCAA GGACAAGCCCATTGGCTGCTCCATCGCCATTAGCACTTACTCTATGCTGGGCCACACCACCAAAAGGTCA TGGGAAGCTGAGAGAGTCATGGAATGGCTCAAAACCCAGGAGTGGGGCCTCATGATCCTTGACGAGGTGC ACACCATTCCAGCCAAGATGTTCCGGCGAGTGCTGACCATTGTGCAGGCCCACTGTAAGCTTGGTTTGAC TGCAACCCTCGTCCGGGAAGATGACAAAATTGTTGACTTAAATTTCTTGATCGGACCCAAGCTTTACGAA GCCAACTGGATGGAGCTGCAGAACAATGGGTACATCGCCAAAGTCCAGTGTGCTGAGGTCTGGTGCCCGA TGTCTCCTGAGTTCTACCGAGAGTATGTGGCAATCAAAACAAAGAAACGCATCCTGTTGTACACCATGAA TCCCAACAAATTCAGAGCCTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTC TTTGCTGACAATGTGTTTGCCTTGAAGGAATATGCTATTCGGCTGAACAAACCTTACATCTATGGGCCCA CGTCCCAGGGAGAAAGGATGCAGATTCTCCAGAACTTCAAACACAACCCCAAAATCAACACCATCTTCAT CTCTAAGGTTGGTGACACATCCTTTGATCTGCCAGAAGCAAATGTCCTCATTCAGATCTCTTCCCATGGT GGCTCCAGACGGCAGGAGGCGCAGAGACTGGGGCGGGTACTCAGAGCCAAGAAAGGGATGGTCGCAGAGG AGTACAATGCCTTTTTCTACTCCCTGGTGTCCCAGGACACACAGGAAATGGCTTATTCTACCAAGCGACA GAGATTCTTAGTAGACCAGGGTTACAGCTTTAAGGTAATCACAAAGCTAGCCGGCATGGAGGAAGAGGAG CTGGCATTCTCCACCAAAGAGGAGCAGCAGCAGCTCCTGCAGAAGGTCCTGGCAGCCACTGACCTGGACG CAGAGGAGGAAGTGGTGGCTGGAGAGTTTGGCTCTAGATCTGGCCAGGCATCCCGGCGCTTTGGCACCAT GAGCTCTCTGTCCGGGGCAGACGATACTGTGTATATGGAGTACCACTCCTCCCGAAACAAAGCCTCCACC AAGCACGTGCACCCACTTTTCAAACGCTTCAGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR215076 representing NM_001031644
Red=Cloning site Green=Tags(s) MGKRDRVDRDKKKSKKRQYEEEEEDEDDAPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGD HTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYL RKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELI TETFTSKSAISKTVEGSGGASTSQGVDAQAKSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEEL QKRCICLEYPLLAEYDFRNDSLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLVG VTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSIAISTYSMLGHTTKRS WEAERVMEWLKTQEWGLMILDEVHTIPAKMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLYE ANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIV FADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHG GSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEE LAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRFGTMSSLSGADDTVYMEYHSSRNKAST KHVHPLFKRFRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031644 |
| ORF Size | 2346 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031644.1, NP_001026814.1 |
| RefSeq Size | 2643 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 291703 |
| UniProt ID | Q4G005 |
| MW | 89.1 kDa |
| Gene Summary | ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN215076 | Ercc3 (untagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 10,070.00 |
|
| RR215076L3 | Lenti ORF clone of Ercc3 (Myc-DDK-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 11,880.00 |
|
| RR215076L4 | Lenti ORF clone of Ercc3 (mGFP-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 13,020.00 |
