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Ercc3 (NM_001031644) Rat Untagged Clone
CAT＃: RN215076
Ercc3 (untagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug)
CNY 10,070.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC112916 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN215076 representing NM_001031644
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAAAAGAGATCGAGTGGACCGCGACAAGAAGAAATCCAAGAAGAGGCAGTATGAAGAGGAAGAGG AAGACGAAGACGACGCTCCTGGGAACGAGTCTCAGGAAGCGGTGCCCTCAGCCGCTGGGAAACAGGTGGA CGAATCCAGCACCAAAGTGGATGAATATGGAGCAAAGGACTACAGACAGCAGATGCCACTAAAGGGTGAC CATACTTCTAGGCCCCTCTGGGTGGCTCCTGATGGCCACATTTTCTTGGAAGCCTTCTCTCCAGTTTACA AATATGCCCAAGACTTCCTGGTGGCAATTGCAGAGCCAGTGTGCCGGCCCACGCATGTACACGAATACAA GCTGACCGCCTACTCCCTCTATGCAGCTGTCAGTGTTGGGCTGCAGACCAGTGACATCACCGAGTACCTC AGAAAGCTCAGTAAGACTGGAGTTCCTGATGGAATCATCCAGTTTATTAAGCTGTGCACTGTCAGCTACG GAAAAGTCAAGCTGGTCCTGAAGCACAACAGGTACTTTGTTGAAAGTTCTCACCCTGATGTCATCCAGCA TCTTCTTCAAGACCCAGTAATACGGGAATGTCGCTTGAGGAATGCCGAGGGAGAGGCTACTGAACTCATC ACAGAGACGTTTACGAGCAAATCTGCTATTTCTAAGACTGTGGAAGGCAGTGGTGGGGCTTCTACTTCAC AGGGGGTAGATGCGCAAGCCAAATCTGACATCCCCAAAGACCTGTTTGATTTTTATGAGCAAATGGACAA GGATGAGGAGGAGGAGGAAGAGACACAGACAGTCTCCTTTGAAGTTAAGCAGGAGATGATCGAGGAGCTC CAGAAGCGCTGCATCTGCCTAGAGTACCCGCTGTTGGCAGAGTATGACTTCCGGAATGACTCTCTGAACC CCGATATCAACATTGACCTGAAGCCCACAGCCGTGCTCAGGCCCTATCAGGAGAAGAGCCTGCGCAAGAT GTTTGGCAATGGGCGAGCACGCTCAGGAGTCATTGTTCTTCCCTGTGGTGCTGGGAAGTCCCTGGTGGGC GTGACTGCCGCGTGTACTGTCAGAAAGCGCTGTCTGGTCCTGGGCAACTCGGCTGTGTCTGTGGAGCAGT GGAAAGCCCAGTTTAAGATGTGGTCAACCATCGATGACAGCCAGATCTGCCGCTTCACCTCAGATGCCAA GGACAAGCCCATTGGCTGCTCCATCGCCATTAGCACTTACTCTATGCTGGGCCACACCACCAAAAGGTCA TGGGAAGCTGAGAGAGTCATGGAATGGCTCAAAACCCAGGAGTGGGGCCTCATGATCCTTGACGAGGTGC ACACCATTCCAGCCAAGATGTTCCGGCGAGTGCTGACCATTGTGCAGGCCCACTGTAAGCTTGGTTTGAC TGCAACCCTCGTCCGGGAAGATGACAAAATTGTTGACTTAAATTTCTTGATCGGACCCAAGCTTTACGAA GCCAACTGGATGGAGCTGCAGAACAATGGGTACATCGCCAAAGTCCAGTGTGCTGAGGTCTGGTGCCCGA TGTCTCCTGAGTTCTACCGAGAGTATGTGGCAATCAAAACAAAGAAACGCATCCTGTTGTACACCATGAA TCCCAACAAATTCAGAGCCTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATTGTC TTTGCTGACAATGTGTTTGCCTTGAAGGAATATGCTATTCGGCTGAACAAACCTTACATCTATGGGCCCA CGTCCCAGGGAGAAAGGATGCAGATTCTCCAGAACTTCAAACACAACCCCAAAATCAACACCATCTTCAT CTCTAAGGTTGGTGACACATCCTTTGATCTGCCAGAAGCAAATGTCCTCATTCAGATCTCTTCCCATGGT GGCTCCAGACGGCAGGAGGCGCAGAGACTGGGGCGGGTACTCAGAGCCAAGAAAGGGATGGTCGCAGAGG AGTACAATGCCTTTTTCTACTCCCTGGTGTCCCAGGACACACAGGAAATGGCTTATTCTACCAAGCGACA GAGATTCTTAGTAGACCAGGGTTACAGCTTTAAGGTAATCACAAAGCTAGCCGGCATGGAGGAAGAGGAG CTGGCATTCTCCACCAAAGAGGAGCAGCAGCAGCTCCTGCAGAAGGTCCTGGCAGCCACTGACCTGGACG CAGAGGAGGAAGTGGTGGCTGGAGAGTTTGGCTCTAGATCTGGCCAGGCATCCCGGCGCTTTGGCACCAT GAGCTCTCTGTCCGGGGCAGACGATACTGTGTATATGGAGTACCACTCCTCCCGAAACAAAGCCTCCACC AAGCACGTGCACCCACTTTTCAAACGCTTCAGGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001031644 |
| Insert Size | 2349 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001031644.1, NP_001026814.1 |
| RefSeq Size | 2643 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 291703 |
| UniProt ID | Q4G005 |
| Gene Summary | ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR215076 | Ercc3 (Myc-DDK-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 10,070.00 |
|
| RR215076L3 | Lenti ORF clone of Ercc3 (Myc-DDK-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 11,880.00 |
|
| RR215076L4 | Lenti ORF clone of Ercc3 (mGFP-tagged ORF) - Rat excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3), (10 ug) |
CNY 13,020.00 |
