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Dlst (NM_001006981) Rat Tagged ORF Clone
CAT＃: RR214067
- TrueORF®
Dlst (Myc-DDK-tagged ORF) - Rat dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10 ug)
"NM_001006981" in other vectors (3)
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CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC95077 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR214067 representing NM_001006981
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTCCCGGTCCCGCTGCGTGTCCCGGGCGTTCAGCCGCTCGCTTTCTGCCTTCCAGAAGGGGAACT GCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGTCAGGGACCAGGTTACCCTGACAGCAGGAA GATGGTCATTAACAACAGTAGCGTCTTCAGTGTTCGCTTCTTCCAAACCACGGCAGTGTGCAAGAATGAT GTGATTACAGTCCAGACCCCAGCGTTTGCAGAGTCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTG TTGGAGATGCAGTTGCAGAAGATGAAGTGGTGTGTGAGATTGAGACAGACAAGACTTCTGTGCAGGTTCC ATCACCAGCAAATGGCATCATTGAAGCTCTTTTGGTACCCGATGGGGGCAAAGTTGAAGGAGGAACTCCT CTATTCACACTCAGGAAAACCGGTGCTGCTCCTGCTAAGGCCAAACCAGCTGAAGCCCCTGCTACAGCCC ACAAAGCAGCGCCTGAAGCACCGGCGGCCCCTCCTCCTCCTGTAGCACCAGTGCCCACTCAGATGCCACC TGTGCCCTCACCCTCACAACCTCCTTCTAGCAAACCAGTGTCTGCAATAAAACCCACTGCTGCCCCTCCA CTGGCTGAGGCGGGAGCTGCTAAAGGCCTGCGCTCAGAACATCGGGAAAAGATGAACAGGATGCGGCAGC GCATCGCCCAGCGTCTGAAGGAAGCCCAGAACACCTGCGCAATGCTGACGACGTTCAATGAGGTTGACAT GAGTAACATACAAGAGATGAGAGCTCGGCACAAAGATGCTTTCCTGAAAAAACATAACCTGAAATTAGGC TTCATGTCGGCATTTGTGAAGGCCTCAGCATTCGCCTTGCAGGAGCAGCCTGTAGTAAACGCAGTGATTG ATGACGCAACCAAGGAGGTGGTGTACAGAGATTATATTGACATCAGTGTCGCAGTTGCTACCCCAAGGGG TCTCGTGGTTCCTGTCATCAGGAATGTGGAAACTATGAATTATGCAGATATTGAACGGACCATTAATGAA CTAGGAGAGAAGGCCCGGAAGAATGAACTTGCCATCGAAGACATGGATGGGGGCACCTTCACCATCAGCA ATGGAGGAGTTTTCGGCTCACTTTTCGGAACACCCATTATCAACCCGCCTCAGTCTGCCATTCTGGGCAT GCATGGCATCTTCGACAGGCCTGTGGCTGTGGGGGGCAAGGTGGAAGTCCGACCTATGATGTATGTAGCC CTGACCTACGACCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCCGAAAAATCAAGGCAGCAG TAGAAGATCCACGAGTCCTCCTCCTAGACCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR214067 representing NM_001006981
Red=Cloning site Green=Tags(s) MLSRSRCVSRAFSRSLSAFQKGNCPLGRRSLPGVSLCQGPGYPDSRKMVINNSSVFSVRFFQTTAVCKND VITVQTPAFAESVTEGDVRWEKAVGDAVAEDEVVCEIETDKTSVQVPSPANGIIEALLVPDGGKVEGGTP LFTLRKTGAAPAKAKPAEAPATAHKAAPEAPAAPPPPVAPVPTQMPPVPSPSQPPSSKPVSAIKPTAAPP LAEAGAAKGLRSEHREKMNRMRQRIAQRLKEAQNTCAMLTTFNEVDMSNIQEMRARHKDAFLKKHNLKLG FMSAFVKASAFALQEQPVVNAVIDDATKEVVYRDYIDISVAVATPRGLVVPVIRNVETMNYADIERTINE LGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPIINPPQSAILGMHGIFDRPVAVGGKVEVRPMMYVA LTYDHRLIDGREAVTFLRKIKAAVEDPRVLLLDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001006981 |
| ORF Size | 1362 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001006981.2, NP_001006982.2 |
| RefSeq Size | 2797 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 299201 |
| UniProt ID | Q01205 |
| MW | 48.9 kDa |
| Gene Summary | Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex (By similarity). The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) (By similarity). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN214067 | Dlst (untagged ORF) - Rat dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10 ug) |
CNY 5,800.00 |
|
| RR214067L3 | Lenti ORF clone of Dlst (Myc-DDK-tagged ORF) - Rat dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10 ug) |
CNY 7,700.00 |
|
| RR214067L4 | Lenti ORF clone of Dlst (mGFP-tagged ORF) - Rat dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10 ug) |
CNY 8,550.00 |
