Dlst (NM_001006981) Rat Untagged Clone

CAT#: RN214067

Dlst (untagged ORF) - Rat dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex) (Dlst), (10 ug)



  "NM_001006981" in other vectors (3)

CNY 5,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC95077
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN214067 representing NM_001006981
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTCCCGGTCCCGCTGCGTGTCCCGGGCGTTCAGCCGCTCGCTTTCTGCCTTCCAGAAGGGGAACT
GCCCTCTAGGGAGACGTTCCCTGCCTGGGGTCTCCTTATGTCAGGGACCAGGTTACCCTGACAGCAGGAA
GATGGTCATTAACAACAGTAGCGTCTTCAGTGTTCGCTTCTTCCAAACCACGGCAGTGTGCAAGAATGAT
GTGATTACAGTCCAGACCCCAGCGTTTGCAGAGTCTGTCACAGAGGGAGATGTCAGGTGGGAGAAAGCTG
TTGGAGATGCAGTTGCAGAAGATGAAGTGGTGTGTGAGATTGAGACAGACAAGACTTCTGTGCAGGTTCC
ATCACCAGCAAATGGCATCATTGAAGCTCTTTTGGTACCCGATGGGGGCAAAGTTGAAGGAGGAACTCCT
CTATTCACACTCAGGAAAACCGGTGCTGCTCCTGCTAAGGCCAAACCAGCTGAAGCCCCTGCTACAGCCC
ACAAAGCAGCGCCTGAAGCACCGGCGGCCCCTCCTCCTCCTGTAGCACCAGTGCCCACTCAGATGCCACC
TGTGCCCTCACCCTCACAACCTCCTTCTAGCAAACCAGTGTCTGCAATAAAACCCACTGCTGCCCCTCCA
CTGGCTGAGGCGGGAGCTGCTAAAGGCCTGCGCTCAGAACATCGGGAAAAGATGAACAGGATGCGGCAGC
GCATCGCCCAGCGTCTGAAGGAAGCCCAGAACACCTGCGCAATGCTGACGACGTTCAATGAGGTTGACAT
GAGTAACATACAAGAGATGAGAGCTCGGCACAAAGATGCTTTCCTGAAAAAACATAACCTGAAATTAGGC
TTCATGTCGGCATTTGTGAAGGCCTCAGCATTCGCCTTGCAGGAGCAGCCTGTAGTAAACGCAGTGATTG
ATGACGCAACCAAGGAGGTGGTGTACAGAGATTATATTGACATCAGTGTCGCAGTTGCTACCCCAAGGGG
TCTCGTGGTTCCTGTCATCAGGAATGTGGAAACTATGAATTATGCAGATATTGAACGGACCATTAATGAA
CTAGGAGAGAAGGCCCGGAAGAATGAACTTGCCATCGAAGACATGGATGGGGGCACCTTCACCATCAGCA
ATGGAGGAGTTTTCGGCTCACTTTTCGGAACACCCATTATCAACCCGCCTCAGTCTGCCATTCTGGGCAT
GCATGGCATCTTCGACAGGCCTGTGGCTGTGGGGGGCAAGGTGGAAGTCCGACCTATGATGTATGTAGCC
CTGACCTACGACCACCGGCTGATTGATGGCAGAGAGGCTGTGACTTTCCTCCGAAAAATCAAGGCAGCAG
TAGAAGATCCACGAGTCCTCCTCCTAGACCTTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001006981
Insert Size 1365 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001006981.2, NP_001006982.2
RefSeq Size 2797 bp
RefSeq ORF 1365 bp
Locus ID 299201
UniProt ID Q01205
Gene Summary Dihydrolipoamide succinyltransferase (E2) component of the 2-oxoglutarate dehydrogenase complex (By similarity). The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) (By similarity). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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