United States
Germany
Japan
United Kingdom
China
St8sia1 (NM_012813) Rat Tagged ORF Clone
CAT＃: RR213865
- TrueORF®
St8sia1 (Myc-DDK-tagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug)
"NM_012813" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Siat8; Siat8a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR213865 representing NM_012813
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCTGCGGGCGGGCCCTACATACATCCAGAGGGGCCATGGCCATGCTGGCGCGGAAATTCCCGC GTACCCGGCTACCCGTGGGAGCCAGTGCCCTCTGTGTGGTGGTCCTCTGTTGGCTCTATGTCTTCCCTGT CTACCGGCTGCCCAACGAGAAGGAGATCGTGCAAGGAGTGCTGGCGCAGCGCACAGCTTGGAGGAGGAAC CAGACGTCAGCCAGGCTCTTCAGGAAACAAATGGAAGACTGCTGCAACCCCGCCCATCTCTTTGCAATGA CGAAGGTGAACTCCCCCATGGGGAAGAGCCTGTGGTATGACGGGGAGTTTCTATACTCGCTCACCATCGA CAACTCCACGTACTCCCTCTTCCCCCAGGCAACCCCCTTCCAGCTGCCATTGAAGAAATGTGCGGTGGTG GGAAACGGTGGGATTCTGAAGATGAGTGGCTGTGGCCGTCAAATAGATGAAGCAAATTTTGTCATGCGGT GTAACCTTCCTCCCTTGTCAAGTGAATACACCAGAGATGTGGGTTCCAAGACTCAGTTGGTGACAGCTAA CCCCAGCATAATTCGCCAGAGATTTGAAAACCTGCTGTGGTCCAGAAAGAAGTTTGTGGACAACATGAAG ATCTATAACCACAGTTACATATACATGCCTGCCTTTTCTATGAAGACGGGAACAGAGCCGTCTCTCCGTG TGTACTACACCCTGAAAGACGCTGGAGCCAATCAAACAGTGCTTTTTGCTAACCCCAACTTTCTTCGAAA CATTGGAAAGTTCTGGAAGGGTAGGGGGATCCATGCCAAGCGCCTGTCTACAGGACTCTTTTTGGTGAGT GCAGCCTTGGGCCTTTGTGAGGAGGTGTCCATCTACGGCTTCTGGCCCTTCTCCGTGAACATGCAAGGGG AACCTATCAGCCACCATTACTATGACAACGTCTTGCCCTTCTCAGGCTTCCATGCCATGCCTGAGGAGTT TCTTCAACTCTGGTATCTTCATAAAATGGGTGCACTGAGGATGCAACTGGACCCATGTGAGGAGCCGTCG CCGCCACAGCCCACTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR213865 representing NM_012813
Red=Cloning site Green=Tags(s) MSPCGRALHTSRGAMAMLARKFPRTRLPVGASALCVVVLCWLYVFPVYRLPNEKEIVQGVLAQRTAWRRN QTSARLFRKQMEDCCNPAHLFAMTKVNSPMGKSLWYDGEFLYSLTIDNSTYSLFPQATPFQLPLKKCAVV GNGGILKMSGCGRQIDEANFVMRCNLPPLSSEYTRDVGSKTQLVTANPSIIRQRFENLLWSRKKFVDNMK IYNHSYIYMPAFSMKTGTEPSLRVYYTLKDAGANQTVLFANPNFLRNIGKFWKGRGIHAKRLSTGLFLVS AALGLCEEVSIYGFWPFSVNMQGEPISHHYYDNVLPFSGFHAMPEEFLQLWYLHKMGALRMQLDPCEEPS PPQPTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012813 |
| ORF Size | 1068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012813.2, NP_036945.2 |
| RefSeq Size | 1472 bp |
| RefSeq ORF | 1071 bp |
| Locus ID | 25280 |
| MW | 40.4 kDa |
| Gene Summary | an alpha-2,8-sialyltransferase that catalyzes the biosynthesis of GT1a and GQ1b as well as GD3 [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN213865 | St8sia1 (untagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug) |
CNY 3,990.00 |
|
| RR213865L3 | Lenti ORF clone of St8sia1 (Myc-DDK-tagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug) |
CNY 6,080.00 |
|
| RR213865L4 | Lenti ORF clone of St8sia1 (mGFP-tagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug) |
CNY 6,650.00 |
其它St8sia1产品
