St8sia1 (NM_012813) Rat Untagged Clone

CAT#: RN213865

St8sia1 (untagged ORF) - Rat ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), (10 ug)



  "NM_012813" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
St8sia1 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Siat8; Siat8a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN213865 representing NM_012813
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCCCTGCGGGCGGGCCCTACATACATCCAGAGGGGCCATGGCCATGCTGGCGCGGAAATTCCCGC
GTACCCGGCTACCCGTGGGAGCCAGTGCCCTCTGTGTGGTGGTCCTCTGTTGGCTCTATGTCTTCCCTGT
CTACCGGCTGCCCAACGAGAAGGAGATCGTGCAAGGAGTGCTGGCGCAGCGCACAGCTTGGAGGAGGAAC
CAGACGTCAGCCAGGCTCTTCAGGAAACAAATGGAAGACTGCTGCAACCCCGCCCATCTCTTTGCAATGA
CGAAGGTGAACTCCCCCATGGGGAAGAGCCTGTGGTATGACGGGGAGTTTCTATACTCGCTCACCATCGA
CAACTCCACGTACTCCCTCTTCCCCCAGGCAACCCCCTTCCAGCTGCCATTGAAGAAATGTGCGGTGGTG
GGAAACGGTGGGATTCTGAAGATGAGTGGCTGTGGCCGTCAAATAGATGAAGCAAATTTTGTCATGCGGT
GTAACCTTCCTCCCTTGTCAAGTGAATACACCAGAGATGTGGGTTCCAAGACTCAGTTGGTGACAGCTAA
CCCCAGCATAATTCGCCAGAGATTTGAAAACCTGCTGTGGTCCAGAAAGAAGTTTGTGGACAACATGAAG
ATCTATAACCACAGTTACATATACATGCCTGCCTTTTCTATGAAGACGGGAACAGAGCCGTCTCTCCGTG
TGTACTACACCCTGAAAGACGCTGGAGCCAATCAAACAGTGCTTTTTGCTAACCCCAACTTTCTTCGAAA
CATTGGAAAGTTCTGGAAGGGTAGGGGGATCCATGCCAAGCGCCTGTCTACAGGACTCTTTTTGGTGAGT
GCAGCCTTGGGCCTTTGTGAGGAGGTGTCCATCTACGGCTTCTGGCCCTTCTCCGTGAACATGCAAGGGG
AACCTATCAGCCACCATTACTATGACAACGTCTTGCCCTTCTCAGGCTTCCATGCCATGCCTGAGGAGTT
TCTTCAACTCTGGTATCTTCATAAAATGGGTGCACTGAGGATGCAACTGGACCCATGTGAGGAGCCGTCG
CCGCCACAGCCCACTTCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012813
Insert Size 1071 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012813.2, NP_036945.2
RefSeq Size 1472 bp
RefSeq ORF 1071 bp
Locus ID 25280
Gene Summary an alpha-2,8-sialyltransferase that catalyzes the biosynthesis of GT1a and GQ1b as well as GD3 [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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