Cilp (NM_001108161) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR213845 representing NM_001108161 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAACCAAGACCTGGGTACTCTCTTTCCTGGTCCTTGAAGTCACCACCGTGCTGGGGAGACAGA TGATGCTTACCCAGTCAGCAAGAAGAGTCCAGCCTGTGAAGAGGACCCCTAAGATCCTCACCAAGCCTGT TAACTCCCAGGAGAGTCCTGGAGAGTGGACGACTTGGTTTAACATCGACCACCCAGGTGGGCAGGGTGAC TATGAGCGACTGGATGCCATTCGATTCTACTACGGGGAGCGCGTGTGCGCCCGTCCCCTCCGGCTAGAGG CTCGGACCACTGACTGGATGCCTGCGGGCATCACTGGCCAGGTGGTCCATGGCAGTCCCCGTGAGGGCTT CTGGTGCCTCAACAAGGAACAGAGACCAGGCCAGAACTGTTCCAATTACACAGTGCGCTTCCTCTGCCCA CCAGGGTCCTTGCGGAGAGATACAGAGCATATCTGGAGTTCCTGGTCTCCCTGGAGCGCGTGCTCAGCCG CCTGTGGTCACACCGGGGTCCAGACCCGTACCCGCACCTGCTTGGCACAGACAGTGTCACTATGTAATGA GGCCACTGAGGAGGGACAGCTCTGCATGAGCCAGGCCTGCACAGCTTGTGACCTGACCTGCCCCATGGGC CAGGTAAATGCTGACTGTGATGCCTGCATGTGTCAGGACTTCATGCTTCACGGGGCCATCTCCCTCCCTG GAGGTGGCCCAGCTCCAGGGGCCACTGTCTACCTGCTGGCTAAGGCACCAAAGATGCTGACCCAAACAGA CAGCAGCGGAAGTTTCCGAGTTCCTGGCTTGTGTCCTGATGGCAAAACCGTCCTGAAAGTTACCAAGAGC AAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTGAAGTCAGCCACCATCAACGCAGAGTTTG TGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGACGAAAGCACGTCGGGCTGGGCAGAGTGTGTC TCTGTGCTGCAAGGCCACAGGGAAACCTAGTCCAGACAAGTACTTCTGGTACCACAACAACACATTGCTG GATCCTTCCCTCTATAAACACCAGAGCAAGCTGGTGCTGAGGAACCTGCAGCAGGACCAAGCAGGGGAGT ACTTCTGTAAGGCGCAGAGTGATGCTGGGGCTGTGAAGTCCAAGGTCACCCAACTCACTGTCATTGCACA CGATGAGGCTCCTTGCAACCCAACCCCAGAGAGCTACCTTATCCGGCTGCCCCATGATTGCTTCCAGAAT GCCACCAACTCCTTCTACTATGATGTAGGCCGCTGCCCCATCAAGACCTGTGCAGGGCAGCAAGACAATG GGATCCGGTGCCAAGATGCCGTGGAGAACTGCTGTGGTATTTCCAGAACAGAGGAGAGGGAGATCCAGTG CAGTGGGTACACACTGCCCACCAAGGTGGCCATGGAGTGCAGCTGTCAACGATGTGCAGAGACACGGAGC ATCGTTCGGGGCCGGGTCACTGCTGCTGACGATGGGGAGCCCATGCGCTTTGGCCACGTGTACATGGGGA ATAACCGTGTGAGCATGACTGGCTACAAAGGCACATTCACCCTCCACGTCCCCCAGGACACGGAGAGGCT GGTGCTCACGTTTGTGGACAGGCTGCAGAAATTTGTCAACACTACCAAAGTGCTGCCCTTCAACAAGAAA GGGAGCGCAGTGTTCCATGAGATCAAGATGCTTCGGCAGAAAGAGCCCATCACGTTGGAAGCCACGGAAA CCAACATTATCCCCCTGGGAGAGGTGGCTGGTGAGGATCCTGTGGCTGAACTGGAGATTCCACCCAAAAG TTTCTATAAGCAGAATGGGGAGCCCTTCACAGGAAAAGTAAAGGCCAGTGTGACCTTCCTGGATCCTCGG AATGTCTCTACGGCCACAGCCACCCAAAGTGACCTGAACTTCATTAATGATGAAGGAGACACCTTTCCCC TTAGAACATACGGGATGTTTTCTGTGGACTTCAGAGATGAGGCCACTTCGGAGTCACTTAATGCTGGCAA GGTGAAGGTCCATCTTGACTCGACCCAGGTCAAAATGCCAGAGCATGCGCGGGCCATGAAACTCTGGTCG CTCAACCCAAACACGGGGCTGTGGGAAGAGGAAGGCGATTTCAAATTTGAAAGCCAGAGGAGGAACAAGA GGGAAGAGAGAACCTTCCTAGTGGGAAATATGGAGATCCGTGAGAGAAGGCTCTTTAACCTGGATGTCCC AGAAAGCAGAAGGTGCTTCATCAAGGTGAGGACTTATCGGAGTGAGAGGTTCCTGCCCAGTGAGCAAATC CAGGGGGTTGTAGTCTCAGTGATCAACCTGGAGCCCAGAACTGGCTTTTCATCTAACCCCAGGGCCTGGG GCCGATTTGACAGTGTCATCACAGGGCCCAATGGAGCCTGCCTGCCTGCCTTCTGTGACGATCAGTCCCC TGATGCCTACTCTGTCTATGTTTTGGCAAGCCTTTCTGGAGAAGAACTAGAGGCAGTTGAGTCTTCTCCT AAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTCAACAAACTTAAATACCGTCGGACAGACC ATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTATGGCCAAACCAAGGCCCAACTCAGCTGA GGAGAGCAATGGGCCCATCTATGCCTTTGAGAACCTTCGGGCATGCGAGGAGGCACCACCCAGTGCAGCT CACTTCCGGTTCTATCAGATTGAGGGGGATCGCTATGACTATAACACAGTTCCTTTCAATGAAGATGACC CTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGCCAATGGAGTTCAGAGCCTGCTACATCAA GGTAAAGATTGTGGGGCCACTTGAGGTGAATGTACGATCCCGTAACATGGGCGGCACCCACCGGCAGACT GTGGGGAAGCTCTATGGGATCCGGGATGTGAAAAGTACTCGGGACAGAGACCAACCTAATGTCTCATCTG CCTGTCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAGACCGTGTAGACCGCACATTAGTGAAGGT CATCCCCCAGGGCAGCTGCCATCGAGCCAGTGTTAACTCTATGCTGCACGAGTACCTGGTCAACCACCTT CCCCTGGCAGTCAACAATGACACCAGCGAGTATACCATGCTGGCACCCCTGGACCCTCTGGGCCACAATT ACGGTATCTATACTGTCACTGACCAGGACCCTGGTACAGCTAAGGAGATTGCGCTTGGCCGGTGCTTTGA CGGCACTTCTGATGGCTCCTCCAGAATCATGAAGAGCAACGTGGGAGTCGCCCTGACCTTTAACTGTGCA GAAAGGCAGGTAGGCCGCCAGAGCGCCTTCCAGTACCTCCAAAGCACCCCGGCCCGGTCCCCAGCTACAG GCACTGTCCAAGGAAGAGTACCCGCCATGAGGCAACAACGGGCAAGCAGGGGTGGCCTACGCCGGCGTGG AAGCATGGCCCCTCTGAGATTTTCTGGTGTCGCTCAACAACCTCTGAGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR213845 representing NM_001108161 Red=Cloning site Green=Tags(s) MAATKTWVLSFLVLEVTTVLGRQMMLTQSARRVQPVKRTPKILTKPVNSQESPGEWTTWFNIDHPGGQGD YERLDAIRFYYGERVCARPLRLEARTTDWMPAGITGQVVHGSPREGFWCLNKEQRPGQNCSNYTVRFLCP PGSLRRDTEHIWSSWSPWSACSAACGHTGVQTRTRTCLAQTVSLCNEATEEGQLCMSQACTACDLTCPMG QVNADCDACMCQDFMLHGAISLPGGGPAPGATVYLLAKAPKMLTQTDSSGSFRVPGLCPDGKTVLKVTKS KFAPIMITMPKTSLKSATINAEFVRAETPYIVMNPETKARRAGQSVSLCCKATGKPSPDKYFWYHNNTLL DPSLYKHQSKLVLRNLQQDQAGEYFCKAQSDAGAVKSKVTQLTVIAHDEAPCNPTPESYLIRLPHDCFQN ATNSFYYDVGRCPIKTCAGQQDNGIRCQDAVENCCGISRTEEREIQCSGYTLPTKVAMECSCQRCAETRS IVRGRVTAADDGEPMRFGHVYMGNNRVSMTGYKGTFTLHVPQDTERLVLTFVDRLQKFVNTTKVLPFNKK GSAVFHEIKMLRQKEPITLEATETNIIPLGEVAGEDPVAELEIPPKSFYKQNGEPFTGKVKASVTFLDPR NVSTATATQSDLNFINDEGDTFPLRTYGMFSVDFRDEATSESLNAGKVKVHLDSTQVKMPEHARAMKLWS LNPNTGLWEEEGDFKFESQRRNKREERTFLVGNMEIRERRLFNLDVPESRRCFIKVRTYRSERFLPSEQI QGVVVSVINLEPRTGFSSNPRAWGRFDSVITGPNGACLPAFCDDQSPDAYSVYVLASLSGEELEAVESSP KFNPNAIGVPQPYLNKLKYRRTDHEDPRVKKTAFQISMAKPRPNSAEESNGPIYAFENLRACEEAPPSAA HFRFYQIEGDRYDYNTVPFNEDDPMSWTEDYLAWWPKPMEFRACYIKVKIVGPLEVNVRSRNMGGTHRQT VGKLYGIRDVKSTRDRDQPNVSSACLEFKCSGMLYDQDRVDRTLVKVIPQGSCHRASVNSMLHEYLVNHL PLAVNNDTSEYTMLAPLDPLGHNYGIYTVTDQDPGTAKEIALGRCFDGTSDGSSRIMKSNVGVALTFNCA ERQVGRQSAFQYLQSTPARSPATGTVQGRVPAMRQQRASRGGLRRRGSMAPLRFSGVAQQPLSN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108161
ORF Size	3552 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108161.1, NP_001101631.1
RefSeq Size	4131 bp
RefSeq ORF	3555 bp
Locus ID	315761
MW	132.1 kDa

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