Cilp (NM_001108161) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213845 representing NM_001108161 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAACCAAGACCTGGGTACTCTCTTTCCTGGTCCTTGAAGTCACCACCGTGCTGGGGAGACAGA TGATGCTTACCCAGTCAGCAAGAAGAGTCCAGCCTGTGAAGAGGACCCCTAAGATCCTCACCAAGCCTGT TAACTCCCAGGAGAGTCCTGGAGAGTGGACGACTTGGTTTAACATCGACCACCCAGGTGGGCAGGGTGAC TATGAGCGACTGGATGCCATTCGATTCTACTACGGGGAGCGCGTGTGCGCCCGTCCCCTCCGGCTAGAGG CTCGGACCACTGACTGGATGCCTGCGGGCATCACTGGCCAGGTGGTCCATGGCAGTCCCCGTGAGGGCTT CTGGTGCCTCAACAAGGAACAGAGACCAGGCCAGAACTGTTCCAATTACACAGTGCGCTTCCTCTGCCCA CCAGGGTCCTTGCGGAGAGATACAGAGCATATCTGGAGTTCCTGGTCTCCCTGGAGCGCGTGCTCAGCCG CCTGTGGTCACACCGGGGTCCAGACCCGTACCCGCACCTGCTTGGCACAGACAGTGTCACTATGTAATGA GGCCACTGAGGAGGGACAGCTCTGCATGAGCCAGGCCTGCACAGCTTGTGACCTGACCTGCCCCATGGGC CAGGTAAATGCTGACTGTGATGCCTGCATGTGTCAGGACTTCATGCTTCACGGGGCCATCTCCCTCCCTG GAGGTGGCCCAGCTCCAGGGGCCACTGTCTACCTGCTGGCTAAGGCACCAAAGATGCTGACCCAAACAGA CAGCAGCGGAAGTTTCCGAGTTCCTGGCTTGTGTCCTGATGGCAAAACCGTCCTGAAAGTTACCAAGAGC AAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTGAAGTCAGCCACCATCAACGCAGAGTTTG TGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGACGAAAGCACGTCGGGCTGGGCAGAGTGTGTC TCTGTGCTGCAAGGCCACAGGGAAACCTAGTCCAGACAAGTACTTCTGGTACCACAACAACACATTGCTG GATCCTTCCCTCTATAAACACCAGAGCAAGCTGGTGCTGAGGAACCTGCAGCAGGACCAAGCAGGGGAGT ACTTCTGTAAGGCGCAGAGTGATGCTGGGGCTGTGAAGTCCAAGGTCACCCAACTCACTGTCATTGCACA CGATGAGGCTCCTTGCAACCCAACCCCAGAGAGCTACCTTATCCGGCTGCCCCATGATTGCTTCCAGAAT GCCACCAACTCCTTCTACTATGATGTAGGCCGCTGCCCCATCAAGACCTGTGCAGGGCAGCAAGACAATG GGATCCGGTGCCAAGATGCCGTGGAGAACTGCTGTGGTATTTCCAGAACAGAGGAGAGGGAGATCCAGTG CAGTGGGTACACACTGCCCACCAAGGTGGCCATGGAGTGCAGCTGTCAACGATGTGCAGAGACACGGAGC ATCGTTCGGGGCCGGGTCACTGCTGCTGACGATGGGGAGCCCATGCGCTTTGGCCACGTGTACATGGGGA ATAACCGTGTGAGCATGACTGGCTACAAAGGCACATTCACCCTCCACGTCCCCCAGGACACGGAGAGGCT GGTGCTCACGTTTGTGGACAGGCTGCAGAAATTTGTCAACACTACCAAAGTGCTGCCCTTCAACAAGAAA GGGAGCGCAGTGTTCCATGAGATCAAGATGCTTCGGCAGAAAGAGCCCATCACGTTGGAAGCCACGGAAA CCAACATTATCCCCCTGGGAGAGGTGGCTGGTGAGGATCCTGTGGCTGAACTGGAGATTCCACCCAAAAG TTTCTATAAGCAGAATGGGGAGCCCTTCACAGGAAAAGTAAAGGCCAGTGTGACCTTCCTGGATCCTCGG AATGTCTCTACGGCCACAGCCACCCAAAGTGACCTGAACTTCATTAATGATGAAGGAGACACCTTTCCCC TTAGAACATACGGGATGTTTTCTGTGGACTTCAGAGATGAGGCCACTTCGGAGTCACTTAATGCTGGCAA GGTGAAGGTCCATCTTGACTCGACCCAGGTCAAAATGCCAGAGCATGCGCGGGCCATGAAACTCTGGTCG CTCAACCCAAACACGGGGCTGTGGGAAGAGGAAGGCGATTTCAAATTTGAAAGCCAGAGGAGGAACAAGA GGGAAGAGAGAACCTTCCTAGTGGGAAATATGGAGATCCGTGAGAGAAGGCTCTTTAACCTGGATGTCCC AGAAAGCAGAAGGTGCTTCATCAAGGTGAGGACTTATCGGAGTGAGAGGTTCCTGCCCAGTGAGCAAATC CAGGGGGTTGTAGTCTCAGTGATCAACCTGGAGCCCAGAACTGGCTTTTCATCTAACCCCAGGGCCTGGG GCCGATTTGACAGTGTCATCACAGGGCCCAATGGAGCCTGCCTGCCTGCCTTCTGTGACGATCAGTCCCC TGATGCCTACTCTGTCTATGTTTTGGCAAGCCTTTCTGGAGAAGAACTAGAGGCAGTTGAGTCTTCTCCT AAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTCAACAAACTTAAATACCGTCGGACAGACC ATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTATGGCCAAACCAAGGCCCAACTCAGCTGA GGAGAGCAATGGGCCCATCTATGCCTTTGAGAACCTTCGGGCATGCGAGGAGGCACCACCCAGTGCAGCT CACTTCCGGTTCTATCAGATTGAGGGGGATCGCTATGACTATAACACAGTTCCTTTCAATGAAGATGACC CTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGCCAATGGAGTTCAGAGCCTGCTACATCAA GGTAAAGATTGTGGGGCCACTTGAGGTGAATGTACGATCCCGTAACATGGGCGGCACCCACCGGCAGACT GTGGGGAAGCTCTATGGGATCCGGGATGTGAAAAGTACTCGGGACAGAGACCAACCTAATGTCTCATCTG CCTGTCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAGACCGTGTAGACCGCACATTAGTGAAGGT CATCCCCCAGGGCAGCTGCCATCGAGCCAGTGTTAACTCTATGCTGCACGAGTACCTGGTCAACCACCTT CCCCTGGCAGTCAACAATGACACCAGCGAGTATACCATGCTGGCACCCCTGGACCCTCTGGGCCACAATT ACGGTATCTATACTGTCACTGACCAGGACCCTGGTACAGCTAAGGAGATTGCGCTTGGCCGGTGCTTTGA CGGCACTTCTGATGGCTCCTCCAGAATCATGAAGAGCAACGTGGGAGTCGCCCTGACCTTTAACTGTGCA GAAAGGCAGGTAGGCCGCCAGAGCGCCTTCCAGTACCTCCAAAGCACCCCGGCCCGGTCCCCAGCTACAG GCACTGTCCAAGGAAGAGTACCCGCCATGAGGCAACAACGGGCAAGCAGGGGTGGCCTACGCCGGCGTGG AAGCATGGCCCCTCTGAGATTTTCTGGTGTCGCTCAACAACCTCTGAGCAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108161
Insert Size	3555 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108161.1, NP_001101631.1
RefSeq Size	4131 bp
RefSeq ORF	3555 bp
Locus ID	315761

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