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Lrrc8a (NM_001024782) Rat Tagged ORF Clone
CAT＃: RR209103
- TrueORF®
Lrrc8a (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug)
"NM_001024782" in other vectors (3)
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CNY 10,360.00
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CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Lrrc8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR209103 representing NM_001024782
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCGGTGACAGAGCTCCGCTACTTCGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGT GGGATGTGTTCACTGACTACATCTCCATTGTCATGCTGATGATTGCTGTCTTTGGGGGGACACTGCAAGT CACCCAGGACAAGATGATCTGCCTACCTTGTAAGTGGGTCACCAAAGACTCCTGCAACGACTCCTTCCGG GGCTGGGGGGCCTCCAGCCCAGAGCCCACTTACCCCAACTCCACAGTCCTGCCTACTCCTGACACGGGCC CCACAGGTATCAAGTATGACCTAGATCGCCACCAGTACAACTACGTGGATGCCGTGTGTTACGAGAACCG CCTGCATTGGTTTGCCAAGTACTTCCCCTACCTCGTGCTCCTGCACACCCTCATCTTCCTGGCCTGCAGC AACTTCTGGTTCAAGTTCCCACGCACCAGCTCGAAGCTGGAGCACTTCGTGTCTATCCTGCTCAAGTGCT TTGATTCGCCATGGACCACACGGGCCCTGTCAGAGACAGTAGTGGAGGAGAGTGACCCCAAGCCAGCCTT CAGCAAGATGAATGGCTCCATGGACAAGAAGTCATCCACGGTCAGTGAGGATGTGGAGGCCACTGTGCCC ATGCTGCAGCGGACCAAGTCACGGATTGAGCAGGGCATCGTGGACCGCTCCGAGACAGGTGTGCTGGACA AGAAGGAAGGGGAGCAGGCGAAGGCACTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGG GGACATCGTGTACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCTTCCTCATCATCTGC TACACCGTGTACTATGTGCACAACATCAAGTTTGACGTGGACTGCACCGTGGACATCGAGAGCCTGACAG GCTACCGCACCTACCGCTGTGCCCACCCTCTGGCCACCCTCTTCAAGATCCTGGCATCCTTCTACATCAG CCTGGTCATCTTCTACGGCCTCATCTGCATGTACACCCTGTGGTGGATGCTCCGGCGCTCCCTCAAGAAG TACTCGTTTGAGTCGATCCGAGAGGAGAGCAGCTACAGCGACATCCCAGACGTCAAGAATGACTTCGCCT TCATGTTGCACCTCATTGACCAGTATGACCCTCTCTACTCAAAGCGCTTCGCCGTCTTCCTGTCTGAGGT GAGTGAGAACAAGCTGCGTCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTGCGCCAGCGGCTC ACCAAAAACGCCCAGGACAAGCTGGAGCTGCACCTCTTCATGCTGAGCGGCATCCCGGACACCGTGTTTG ACCTGCTGGAGTTAGAGGTCCTGAAGCTGGAACTGATCCCCGACGTGACCATCCCGCCCAGCATCGCCCA GCTCACAGGCCTCAAGGAGCTCTGGCTGTACCACACGGCAGCCAAGATCGAGGCTCCCGCCCTGGCCTTC CTGCGAGAGAACCTGCGGGCGCTGCACATCAAGTTCACTGACATCAAGGAGATCCCACTGTGGATCTACA GCCTGAAGACACTGGAGGAGCTGCACTTGACAGGCAACCTGAGCGCAGAGAACAACCGCTACATCGTCAT TGACGGACTGCGGGAGCTCAAACGCCTTAAGGTGCTGCGGCTCAAAAGCAACCTGAGCAAGCTGCCGCAG GTGGTCACGGACGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATCGTCC TCAACAGCCTCAAGAAGATGGTTAACCTGACAGAGCTGGAGCTGATCCGCTGCGACCTGGAGCGTATCCC TCACTCCATCTTTAGCCTCCACAACCTGCAGGAGATCGACCTGAAGGACAACAACCTCAAGACCATCGAG GAGATCATCAGCTTCCAGCATCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACA TCCCCATCCAGATCGGCAACCTCACCAACCTTGAGCGCCTCTACCTGAACCGCAACAAAATCGAGAAAAT TCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTGCTC CCCGCTGACATTGGCCTCTTGCAGAACCTCCAGAACCTGGCCGTCACAGCCAATAGGATCGAGGCGCTCC CCCCGGAGCTCTTCCAGTGTCGGAAGCTGCGGGCTCTACACCTGGGCAACAATGTTCTGCAGTCACTGCC CTCGAGAGTGGGTGAGCTGACCAACCTGACGCAGATTGAGCTTCGGGGCAACCGGCTGGAGTGCCTGCCC GTGGAGCTGGGCGAGTGTCCCCTGCTCAAGCGCAGCGGCCTGGTGGTAGAGGAGGACTTGTTCAGCACAC TGCCGCCTGAGGTGAAGGAACGGCTCTGGAGAGCTGACAAGGAGCAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209103 representing NM_001024782
Red=Cloning site Green=Tags(s) MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFR GWGASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACS NFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVP MLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFFLIIC YTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKK YSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRL TKNAQDKLELHLFMLSGIPDTVFDLLELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAF LRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQ VVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIE EIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTLL PADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLP VELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001024782 |
| ORF Size | 2430 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001024782.1, NP_001019953.1 |
| RefSeq Size | 3328 bp |
| RefSeq ORF | 2433 bp |
| Locus ID | 311846 |
| UniProt ID | Q4V8I7 |
| MW | 94.2 kDa |
| Gene Summary | Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28833202). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202). Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (By similarity). Also required for T-cell development (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN209103 | Lrrc8a (untagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 10,360.00 |
|
| RR209103L3 | Lenti ORF clone of Lrrc8a (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 12,260.00 |
|
| RR209103L4 | Lenti ORF clone of Lrrc8a (mGFP-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 13,400.00 |
