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Lrrc8a (NM_001024782) Rat Untagged Clone
CAT＃: RN209103
Lrrc8a (untagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug)
CNY 10,360.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Lrrc8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN209103 representing NM_001024782
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCCGGTGACAGAGCTCCGCTACTTCGCGGACACGCAGCCAGCATACCGGATCCTGAAGCCGTGGT GGGATGTGTTCACTGACTACATCTCCATTGTCATGCTGATGATTGCTGTCTTTGGGGGGACACTGCAAGT CACCCAGGACAAGATGATCTGCCTACCTTGTAAGTGGGTCACCAAAGACTCCTGCAACGACTCCTTCCGG GGCTGGGGGGCCTCCAGCCCAGAGCCCACTTACCCCAACTCCACAGTCCTGCCTACTCCTGACACGGGCC CCACAGGTATCAAGTATGACCTAGATCGCCACCAGTACAACTACGTGGATGCCGTGTGTTACGAGAACCG CCTGCATTGGTTTGCCAAGTACTTCCCCTACCTCGTGCTCCTGCACACCCTCATCTTCCTGGCCTGCAGC AACTTCTGGTTCAAGTTCCCACGCACCAGCTCGAAGCTGGAGCACTTCGTGTCTATCCTGCTCAAGTGCT TTGATTCGCCATGGACCACACGGGCCCTGTCAGAGACAGTAGTGGAGGAGAGTGACCCCAAGCCAGCCTT CAGCAAGATGAATGGCTCCATGGACAAGAAGTCATCCACGGTCAGTGAGGATGTGGAGGCCACTGTGCCC ATGCTGCAGCGGACCAAGTCACGGATTGAGCAGGGCATCGTGGACCGCTCCGAGACAGGTGTGCTGGACA AGAAGGAAGGGGAGCAGGCGAAGGCACTGTTTGAGAAGGTGAAGAAGTTCCGGACCCATGTGGAGGAGGG GGACATCGTGTACCGCCTCTACATGCGGCAGACCATCATCAAGGTGATCAAGTTCTTCCTCATCATCTGC TACACCGTGTACTATGTGCACAACATCAAGTTTGACGTGGACTGCACCGTGGACATCGAGAGCCTGACAG GCTACCGCACCTACCGCTGTGCCCACCCTCTGGCCACCCTCTTCAAGATCCTGGCATCCTTCTACATCAG CCTGGTCATCTTCTACGGCCTCATCTGCATGTACACCCTGTGGTGGATGCTCCGGCGCTCCCTCAAGAAG TACTCGTTTGAGTCGATCCGAGAGGAGAGCAGCTACAGCGACATCCCAGACGTCAAGAATGACTTCGCCT TCATGTTGCACCTCATTGACCAGTATGACCCTCTCTACTCAAAGCGCTTCGCCGTCTTCCTGTCTGAGGT GAGTGAGAACAAGCTGCGTCAGCTGAACCTCAACAACGAGTGGACGCTGGACAAGCTGCGCCAGCGGCTC ACCAAAAACGCCCAGGACAAGCTGGAGCTGCACCTCTTCATGCTGAGCGGCATCCCGGACACCGTGTTTG ACCTGCTGGAGTTAGAGGTCCTGAAGCTGGAACTGATCCCCGACGTGACCATCCCGCCCAGCATCGCCCA GCTCACAGGCCTCAAGGAGCTCTGGCTGTACCACACGGCAGCCAAGATCGAGGCTCCCGCCCTGGCCTTC CTGCGAGAGAACCTGCGGGCGCTGCACATCAAGTTCACTGACATCAAGGAGATCCCACTGTGGATCTACA GCCTGAAGACACTGGAGGAGCTGCACTTGACAGGCAACCTGAGCGCAGAGAACAACCGCTACATCGTCAT TGACGGACTGCGGGAGCTCAAACGCCTTAAGGTGCTGCGGCTCAAAAGCAACCTGAGCAAGCTGCCGCAG GTGGTCACGGACGTGGGCGTGCACCTGCAGAAGCTGTCCATCAACAATGAGGGCACCAAGCTCATCGTCC TCAACAGCCTCAAGAAGATGGTTAACCTGACAGAGCTGGAGCTGATCCGCTGCGACCTGGAGCGTATCCC TCACTCCATCTTTAGCCTCCACAACCTGCAGGAGATCGACCTGAAGGACAACAACCTCAAGACCATCGAG GAGATCATCAGCTTCCAGCATCTGCACCGCCTCACCTGCCTTAAGCTGTGGTACAACCACATCGCCTACA TCCCCATCCAGATCGGCAACCTCACCAACCTTGAGCGCCTCTACCTGAACCGCAACAAAATCGAGAAAAT TCCCACCCAGCTCTTCTACTGCCGCAAGCTGCGCTACCTGGACCTCAGCCACAACAACCTGACCTTGCTC CCCGCTGACATTGGCCTCTTGCAGAACCTCCAGAACCTGGCCGTCACAGCCAATAGGATCGAGGCGCTCC CCCCGGAGCTCTTCCAGTGTCGGAAGCTGCGGGCTCTACACCTGGGCAACAATGTTCTGCAGTCACTGCC CTCGAGAGTGGGTGAGCTGACCAACCTGACGCAGATTGAGCTTCGGGGCAACCGGCTGGAGTGCCTGCCC GTGGAGCTGGGCGAGTGTCCCCTGCTCAAGCGCAGCGGCCTGGTGGTAGAGGAGGACTTGTTCAGCACAC TGCCGCCTGAGGTGAAGGAACGGCTCTGGAGAGCTGACAAGGAGCAGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024782 |
| Insert Size | 2433 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024782.1, NP_001019953.1 |
| RefSeq Size | 3328 bp |
| RefSeq ORF | 2433 bp |
| Locus ID | 311846 |
| UniProt ID | Q4V8I7 |
| Gene Summary | Essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:28833202). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (By similarity). Mediates efflux of amino acids, such as aspartate and glutamate, in response to osmotic stress (PubMed:28833202). Required for in vivo channel activity, together with at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:28833202). Can form functional channels by itself (in vitro). Involved in B-cell development: required for the pro-B cell to pre-B cell transition (By similarity). Also required for T-cell development (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Cryo-EM structures of an LRRC8 chimera with native functional properties reveal heptameric assembly
,null,
eLife
,PubMed ID 36897307
[Lrrc8a]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR209103 | Lrrc8a (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 10,360.00 |
|
| RR209103L3 | Lenti ORF clone of Lrrc8a (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 12,260.00 |
|
| RR209103L4 | Lenti ORF clone of Lrrc8a (mGFP-tagged ORF) - Rat leucine rich repeat containing 8 family, member A (Lrrc8a), (10 ug) |
CNY 13,400.00 |
