Nfat5 (NM_001107425) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NF-AT5; Nfat; TonEBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208725 representing NM_001107425 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC GAGATTCTCTGAAGTTACACCCATCACAGAATTTTCATAGAGCTGGACTATTGGAAGAATCTGTCTATGA TCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGCAGCATCAATGAGTCAGACAAGC GGTGGCGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCCGATGCTTCTTCAGCTCCCTCCTCCTCCA TGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAG CAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCAG TTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCACCAC CACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGCA GAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGAT AACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATT GTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCT TACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGAGTA AAGAAGAGTCCTATGTTGTGTGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGGTAC AGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCCGTAAAAGATAGAACACA GCAAGGCTTTCCTACGGTGAAGCTGGAAGGTCATAATGAGCCAGTGGTGTTGCAGGTGTTTGTGGGCAAT GACTCTGGTCGAGTAAAGCCACACGGATTCTATCAGGCCTGCAGAGTGACCGGGCGAAACACAACTCCGT GCAAAGAAGTGGACATCGAAGGCACCACTGTGATAGAGGTTGGCCTCGACCCTAGCAGCAACATGACGCT GGCAGTGGACTGTGTAGGAATACTGAAGCTGAGAAATGCTGATGTTGAGGCCAGAATTGGAATTGCTGGT TCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACGAGGAAAGATGGCTCTACTC TGACATTGCAGACACCTTCTTCCCCAATTTTATGCACTCAGCCAGCAGGAGTTCCTGAGATCTTAAAGAA AAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTGTTTTTAATTGGGAAGAACTTTCTGAAAGGA ACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCAGAAGCTGAAATTGACATGG AATTGTTCCATCAGAACCATCTTATTGTGAAAGTTCCTCCATATCATGACCAACATATAACTTTGCCTGT ATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCAGCCATTTACTTACACTCCA GATCCAGCAGCTGGTGCTTTGAGTGTAAATGTGAAAAAGGAGATATCTAGTCCAGCAAGACCTTGCTCTT TTGAAGAAGCCTTGAAAGCAATGAAAACGACTGGCTGTAACGTAGATAAGGTAACTATCCTTCCTAATGC CCTGATCACTCCACTCATATCGAGCAGCATGATTAAGACTGAAGATGTTACTCCAATGGAAGTAACATCA GAGAAAAGATCTTCCCCAATTTTTCAGACTACAAAGACAGTCGGATCAACCCAGCAAACTTTAGAAACTA TTTCTAACATAGCGGGAAATGCATCCTTTTCATCACCATCATCTTCTCACTTATCTCCTGAAAATGAAAA TCAGCAGCAGCTTCAGCCCAAGGCATACAACCCGGAGACTCTGACAACTATCCAAACGCAAGACATCTCA CAGCCAGGGACTTTTCCAGCGGTTTCTGCTTCTAGTCAGCTGCCCAGCAGCGATGCACTGCTGCAGCAGG CGGCACAGTTTCAGACAAGAGACGCTCAGTCCAGAGACACAATGCAGTCAGACTCAGTGGTTAACTTGTC ACAGTTGACTGAGGCACCACAGCAGCAGCAGTCCCCACTGCAAGAACAAGCACAGATACCATCAAATATT TTTCCGTCGCCAAATAGTGTGAGCCAGCTCCAGAGTACTATTCAGCAGCTGCAAGCAGGAAGTTTTACAG GCAGCACTGCTAGTGGCAGCAATGGAAGTGTTGACTTGGTCCAGCAGGTTTTAGAGGCACAACAACAGTT ATCGTCTGTTCTGTTTTCTACTCCAGATGGTAATGAGAATGTTCAAGAACAGCTGAATGCAGACATTTTC CAAGTCAGTCAAATTCAAAATAGTGTAAGCCCTGGAATGTTTTCCTCAACAGAGTCTGCAGTTCACACCA GACCAGATAACTTACTACCTGGGAGGGCCGACAGCGTCCATCAGCAGACTGAAAACACACTGTCTAGTCA GCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAAGTGATAGAGTCTTCAGCTGCCATGGTG ATGGAGATGCAGCAGAGCATTTGCCAAGCAGCAGCCCAGATTCAGTCAGAACTGTTTCCTTCAGCTGCTT CAGCAAATGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTCACATGATGAGCGCCCTGCCTAC CAGCGAGGACATGCAAATGCAGTGTGAATTGTTCTCTTCCCCCCCTGCAGTTTCTGGAAATGAAACATCC ACCACCACCACACCGCAGGTGGCAACCCCCGGCTCTACTATGTTTCAGCCGCCAAATTCAGGCGATGGAG AAGAAACGGGAGCACAAGCAAAACAGATTCAGAGCAGTGTCTTTCAGACAATGGTCCAGATGCAGCACAG TGGGGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAACATAATGAGTGTTCAGAGTAATGGT ACCCAGCAACAAGGGAATAGCTTGTTCCAGCAGGGGAGCGAGATGCTGTCACTTCAGTCTGGGAGCTTCT TGCAGCAGTCTTCTCATTCCCAGGCTCAGCTCTTTCACCCTCAGAACCCTATTGCTGATGCTCAGAGCCT TTCCCAGGAGACTCAAGGTCCAATGTTTCATAGCGCCAATCCCATCGTCCACAGTCAGACTTCCACAGCA TCCTCCGAACAGCTGCAGCCTTCCATGTTTCACTCGCAGAGTACCATTGCTGTACTACAGGGCTCTTCAG TCCCTCAGGACCAGCAGTCACCCAACATATATCTTTCCCAAAGTTCCATCAGTAATCTTCAAACTAACAC AGTAGCCCAAGAAGAGCAGATTTCATTTTTTTCAGCACAGAACTCAATTTCTCCACTTCAGTCAACATCA AACACTGAGCAGCAAGCTGCTTTTCAACAGCAGCCTCCAATCTCGCACATCCAGACCCCTCTTCTTTCCC AGGAACAGGCACAGCCCTCTCAGCAAGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCTGCTAA TCCAATGCCTCAGAACCAGCAAGGCCCAATTTTCCAGACACAGCGCCCAATAGTTGGCATGCAGAGTAAC TCTCCATCCCAGGAGCAGCAGCAGCAGCAACAGCAGCAGCAACAACAACAGCAGCAGCAGCAGCAGCAGA GCATTTTATTTAGTAATCAGAATGCCATGGCTACAATGGCCTCCCAGAAGCAGCCACCACCAAACATGAT ATTTAGCCCAAACCAGAACCCGATGGCTAGTCAAGAGCAGCAGAACCAGTCAATTTTTCACCAGCAAAGT AATATGGCCCCAATGAACCAAGAGCAGCAGCCCATGCAGTTTCAGAATCAGCCTACGGTTTCCTCACTTC AGAACCCAGGTCCTACGCCATCTGAGTCACCACAGACCTCCTTGTTCCATAGTTCTCCTCAGATCCAGTT GGTTCAAGGGTCACCTAGTTCTCAAGAGCAACAAGTAACACTCTTCCTCTCTCCAGCATCCATGTCTGCA TTGCAAACCAGTATAAACCAACCAGACATGCAGCAGTCTCCTCTTTATTCCCCACAGAACAACATCCCTG GAATCCAAGGAAGCACTTCTTCACCCCAGCCACAGGCTGCTTTATTTCACAACACTACAGGAGGCACAAT AAACCAACTACAAAATTCTCCTGGCTCATCTCAGCAGACTTCAGGAATGTTCTTATTTGGCATTCAAAAT AATTGTAGTCAGCTGTTAACTTCTGGGCCAGCTACATTGCCAGAGCAGCTGATGGCCATAAATCAGCCGG GCCAACCACAAAATGAGGGCCAATCTTCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAATCTGCCCC ACTGGCCTCCTCGGTGAACAACAGTCAGAACATGGAAAAGATCGATTTGCTTGTTTCGTTGCAAAGCCAA GGGAACAATTTAACCGGCTCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208725 representing NM_001107425 Red=Cloning site Green=Tags(s) MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQNFHRAGLLEESVYDLLPKELQLPPPRETSAASMSQTS GGEAGSPPPAVVAADASSAPSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQ LTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYND NTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTGV KKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGN DSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSSNMTLAVDCVGILKLRNADVEARIGIAG SKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKG TKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTP DPAAGALSVNVKKEISSPARPCSFEEALKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVTS EKRSSPIFQTTKTVGSTQQTLETISNIAGNASFSSPSSSHLSPENENQQQLQPKAYNPETLTTIQTQDIS QPGTFPAVSASSQLPSSDALLQQAAQFQTRDAQSRDTMQSDSVVNLSQLTEAPQQQQSPLQEQAQIPSNI FPSPNSVSQLQSTIQQLQAGSFTGSTASGSNGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQEQLNADIF QVSQIQNSVSPGMFSSTESAVHTRPDNLLPGRADSVHQQTENTLSSQQQQQQQQQQQQQQQVIESSAAMV MEMQQSICQAAAQIQSELFPSAASANGSLQQSPVYQQPSHMMSALPTSEDMQMQCELFSSPPAVSGNETS TTTTPQVATPGSTMFQPPNSGDGEETGAQAKQIQSSVFQTMVQMQHSGDSQPQVNLFSSTKNIMSVQSNG TQQQGNSLFQQGSEMLSLQSGSFLQQSSHSQAQLFHPQNPIADAQSLSQETQGPMFHSANPIVHSQTSTA SSEQLQPSMFHSQSTIAVLQGSSVPQDQQSPNIYLSQSSISNLQTNTVAQEEQISFFSAQNSISPLQSTS NTEQQAAFQQQPPISHIQTPLLSQEQAQPSQQGLFQPQVALGSLPANPMPQNQQGPIFQTQRPIVGMQSN SPSQEQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMIFSPNQNPMASQEQQNQSIFHQQS NMAPMNQEQQPMQFQNQPTVSSLQNPGPTPSESPQTSLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSA LQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQAALFHNTTGGTINQLQNSPGSSQQTSGMFLFGIQN NCSQLLTSGPATLPEQLMAINQPGQPQNEGQSSVTTLLSQQMPESAPLASSVNNSQNMEKIDLLVSLQSQ GNNLTGSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107425
ORF Size	4644 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107425.1, NP_001100895.1
RefSeq Size	13556 bp
RefSeq ORF	4647 bp
Locus ID	307820
UniProt ID	D3ZGB1
MW	167.2 kDa
Gene Summary	Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function]

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