Nfat5 (NM_001107425) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NF-AT5; Nfat; TonEBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208725 representing NM_001107425 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC GAGATTCTCTGAAGTTACACCCATCACAGAATTTTCATAGAGCTGGACTATTGGAAGAATCTGTCTATGA TCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGCAGCATCAATGAGTCAGACAAGC GGTGGCGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCCGATGCTTCTTCAGCTCCCTCCTCCTCCA TGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGACAG CAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAGCAG TTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCACCAC CACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGCA GAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGAT AACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGATT GTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCAGCT TACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGAGTA AAGAAGAGTCCTATGTTGTGTGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGGTAC AGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCCGTAAAAGATAGAACACA GCAAGGCTTTCCTACGGTGAAGCTGGAAGGTCATAATGAGCCAGTGGTGTTGCAGGTGTTTGTGGGCAAT GACTCTGGTCGAGTAAAGCCACACGGATTCTATCAGGCCTGCAGAGTGACCGGGCGAAACACAACTCCGT GCAAAGAAGTGGACATCGAAGGCACCACTGTGATAGAGGTTGGCCTCGACCCTAGCAGCAACATGACGCT GGCAGTGGACTGTGTAGGAATACTGAAGCTGAGAAATGCTGATGTTGAGGCCAGAATTGGAATTGCTGGT TCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACGAGGAAAGATGGCTCTACTC TGACATTGCAGACACCTTCTTCCCCAATTTTATGCACTCAGCCAGCAGGAGTTCCTGAGATCTTAAAGAA AAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTGTTTTTAATTGGGAAGAACTTTCTGAAAGGA ACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCAGAAGCTGAAATTGACATGG AATTGTTCCATCAGAACCATCTTATTGTGAAAGTTCCTCCATATCATGACCAACATATAACTTTGCCTGT ATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCAGCCATTTACTTACACTCCA GATCCAGCAGCTGGTGCTTTGAGTGTAAATGTGAAAAAGGAGATATCTAGTCCAGCAAGACCTTGCTCTT TTGAAGAAGCCTTGAAAGCAATGAAAACGACTGGCTGTAACGTAGATAAGGTAACTATCCTTCCTAATGC CCTGATCACTCCACTCATATCGAGCAGCATGATTAAGACTGAAGATGTTACTCCAATGGAAGTAACATCA GAGAAAAGATCTTCCCCAATTTTTCAGACTACAAAGACAGTCGGATCAACCCAGCAAACTTTAGAAACTA TTTCTAACATAGCGGGAAATGCATCCTTTTCATCACCATCATCTTCTCACTTATCTCCTGAAAATGAAAA TCAGCAGCAGCTTCAGCCCAAGGCATACAACCCGGAGACTCTGACAACTATCCAAACGCAAGACATCTCA CAGCCAGGGACTTTTCCAGCGGTTTCTGCTTCTAGTCAGCTGCCCAGCAGCGATGCACTGCTGCAGCAGG CGGCACAGTTTCAGACAAGAGACGCTCAGTCCAGAGACACAATGCAGTCAGACTCAGTGGTTAACTTGTC ACAGTTGACTGAGGCACCACAGCAGCAGCAGTCCCCACTGCAAGAACAAGCACAGATACCATCAAATATT TTTCCGTCGCCAAATAGTGTGAGCCAGCTCCAGAGTACTATTCAGCAGCTGCAAGCAGGAAGTTTTACAG GCAGCACTGCTAGTGGCAGCAATGGAAGTGTTGACTTGGTCCAGCAGGTTTTAGAGGCACAACAACAGTT ATCGTCTGTTCTGTTTTCTACTCCAGATGGTAATGAGAATGTTCAAGAACAGCTGAATGCAGACATTTTC CAAGTCAGTCAAATTCAAAATAGTGTAAGCCCTGGAATGTTTTCCTCAACAGAGTCTGCAGTTCACACCA GACCAGATAACTTACTACCTGGGAGGGCCGACAGCGTCCATCAGCAGACTGAAAACACACTGTCTAGTCA GCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAAGTGATAGAGTCTTCAGCTGCCATGGTG ATGGAGATGCAGCAGAGCATTTGCCAAGCAGCAGCCCAGATTCAGTCAGAACTGTTTCCTTCAGCTGCTT CAGCAAATGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTCACATGATGAGCGCCCTGCCTAC CAGCGAGGACATGCAAATGCAGTGTGAATTGTTCTCTTCCCCCCCTGCAGTTTCTGGAAATGAAACATCC ACCACCACCACACCGCAGGTGGCAACCCCCGGCTCTACTATGTTTCAGCCGCCAAATTCAGGCGATGGAG AAGAAACGGGAGCACAAGCAAAACAGATTCAGAGCAGTGTCTTTCAGACAATGGTCCAGATGCAGCACAG TGGGGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAACATAATGAGTGTTCAGAGTAATGGT ACCCAGCAACAAGGGAATAGCTTGTTCCAGCAGGGGAGCGAGATGCTGTCACTTCAGTCTGGGAGCTTCT TGCAGCAGTCTTCTCATTCCCAGGCTCAGCTCTTTCACCCTCAGAACCCTATTGCTGATGCTCAGAGCCT TTCCCAGGAGACTCAAGGTCCAATGTTTCATAGCGCCAATCCCATCGTCCACAGTCAGACTTCCACAGCA TCCTCCGAACAGCTGCAGCCTTCCATGTTTCACTCGCAGAGTACCATTGCTGTACTACAGGGCTCTTCAG TCCCTCAGGACCAGCAGTCACCCAACATATATCTTTCCCAAAGTTCCATCAGTAATCTTCAAACTAACAC AGTAGCCCAAGAAGAGCAGATTTCATTTTTTTCAGCACAGAACTCAATTTCTCCACTTCAGTCAACATCA AACACTGAGCAGCAAGCTGCTTTTCAACAGCAGCCTCCAATCTCGCACATCCAGACCCCTCTTCTTTCCC AGGAACAGGCACAGCCCTCTCAGCAAGGTTTATTTCAGCCTCAGGTGGCCCTGGGCTCCCTTCCTGCTAA TCCAATGCCTCAGAACCAGCAAGGCCCAATTTTCCAGACACAGCGCCCAATAGTTGGCATGCAGAGTAAC TCTCCATCCCAGGAGCAGCAGCAGCAGCAACAGCAGCAGCAACAACAACAGCAGCAGCAGCAGCAGCAGA GCATTTTATTTAGTAATCAGAATGCCATGGCTACAATGGCCTCCCAGAAGCAGCCACCACCAAACATGAT ATTTAGCCCAAACCAGAACCCGATGGCTAGTCAAGAGCAGCAGAACCAGTCAATTTTTCACCAGCAAAGT AATATGGCCCCAATGAACCAAGAGCAGCAGCCCATGCAGTTTCAGAATCAGCCTACGGTTTCCTCACTTC AGAACCCAGGTCCTACGCCATCTGAGTCACCACAGACCTCCTTGTTCCATAGTTCTCCTCAGATCCAGTT GGTTCAAGGGTCACCTAGTTCTCAAGAGCAACAAGTAACACTCTTCCTCTCTCCAGCATCCATGTCTGCA TTGCAAACCAGTATAAACCAACCAGACATGCAGCAGTCTCCTCTTTATTCCCCACAGAACAACATCCCTG GAATCCAAGGAAGCACTTCTTCACCCCAGCCACAGGCTGCTTTATTTCACAACACTACAGGAGGCACAAT AAACCAACTACAAAATTCTCCTGGCTCATCTCAGCAGACTTCAGGAATGTTCTTATTTGGCATTCAAAAT AATTGTAGTCAGCTGTTAACTTCTGGGCCAGCTACATTGCCAGAGCAGCTGATGGCCATAAATCAGCCGG GCCAACCACAAAATGAGGGCCAATCTTCTGTGACAACACTTCTTTCTCAGCAAATGCCAGAATCTGCCCC ACTGGCCTCCTCGGTGAACAACAGTCAGAACATGGAAAAGATCGATTTGCTTGTTTCGTTGCAAAGCCAA GGGAACAATTTAACCGGCTCCTTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107425
Insert Size	4647 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107425.1, NP_001100895.1
RefSeq Size	13556 bp
RefSeq ORF	4647 bp
Locus ID	307820
UniProt ID	D3ZGB1
Gene Summary	Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function]

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