Brd4 (NM_001100903) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208565 representing NM_001100903 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCT CCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGTCAGCAAATGCAGCCAGCACCAATCCTCCACC CCCAGAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATATCTGCTCAGAGTGGTG CTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCTGTGGATGCCGTCAAGCTGAACC TCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAA CTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAAC AAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAGAAAATCAATGAAC TGCCTACAGAAGAAACCGAGATCATGATAGTCCAGGCAAAGGGACGAGGACGAGGGAGGAAAGAAACAGG GACAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAGATG CCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACCTCA TTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCCCAGCCACTTCAGACTCCTTCACCGGTACCCCCTCA GCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCTCCCATCATTGCGACCACCCCA CAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCCACCACCATCGACCCCA TTCATGAGCCGCCCTCACTGGCCCCGGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAG ACCTGTGAAGCCCCCAAAGAAGGATGTACCAGACTCCCAGCAGCACCCAGGGCCAGAGAAGAGCAGCAAG ATCTCTGAGCAGCTGAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCTAAGAAACATGCTGCCTATG CCTGGCCTTTCTACAAGCCCGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATTAAACA CCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGGGAGTACAGAGATGCCCAGGAGTTTGGT GCTGATGTCCGATTGATGTTTTCCAACTGCTACAAGTACAATCCCCCTGACCATGAAGTGGTAGCCATGG CTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGTTGT TACGGTGTCCTCCCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGCAGC AGTGATAGTTCCTCTGACAGTGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTG AGCTCCAGGAGCAGCTCAAAGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAACAGAACAAACC AAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAATGGAGGAA AATAAAAAAAGCAAAGCCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCAATG TGAGCAAGAAGGAACCAGCACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAAGAGGACAA GTGTAAGCCTATGTCTTATGAGGAGAAGCGACAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAG CTAGGCCGTGTAGTACACATAATTCAGTCGAGGGAACCATCACTTAAAAACTCCAACCCTGATGAGATTG AGATTGATTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCG GAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGATGTGATTGCTGGTTCTTCCAAGATGAAGGGCTTCTCA TCCTCTGAGTCGGAGAGCACCAGCGAATCTAGCTCCTCTGACAGTGAAGACTCTGAAACAGAGATGGCTC CCAAGTCAAAAAAGAAGGGGCACACTGGGAGGGACCAGAAAAAGCATCATCACCATCACCACCCACAGAT GCAGCCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCGCCACCTCCACAGCAGCCTCCTCCACCCCCACCT CCTCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCCCCACCCCCACCACCTCCGCCCTCCATGCCACAGC AGACTGCCCCAGCGATGAAGTCCTCGCCCCCACCCTTCATCACTGCCCAGGTACCCGTCCTGGAACCACA GCTACCAGGCAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACCTGCCGCAGCCAGAG CTGCCTCCTCACCTGCCCCAGCCACCGGAGCACAGCACTCCACCCCATCTCAACCAGCATGCTGTGGTCT CTCCTCCAGCTTTGCACAATGCGCTGCCCCAACAACCATCTCGGCCCAGCAACCGAGCTGCTGCTCTGCC CCCAAAGCCTGCCCGACCCCCAGCTGTGTCCCCTGCCTTGGCCCAGCCCCCCCTGCTCCCACAGCCACCT ATGGCTCAGCCCCCCCAAGTGCTGCTGGAGGATGAAGAGCCGCCTGCCCCACCCCTCACCTCCATGCAGA TGCAGTTGTACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCTTCCGTGAAGGTGCA GTCCCAACCCCCACCACCTTTGCCGCCTCCACCCCACCCTTCTGTGCAGCAACAGCAGCTCCAGCAGCAG CCACCACCACCCCCACCTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCCCCACCACGACCAG TTCACTTGCCATCCATGCCCTTCTCTGCTCACATTCAGCAGCCCCCACCACCCACAGGACAGCAACCCAC CCACCCACCCCCAGGACAGCAGCCACCCCCACCACAGCCTGCCAAGCCTCAGCAAGTCATCCAGCATCAC CCTTCACCCCGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAAGCCCCTTCTCCGCTTA TGATACATTCCCCTCAAATGCCACAGTTCCAGAGCCTGACCCATCAGTCTCCACCCCAGCAAAACGTCCA GCCCAAGAAGCAGGTAAAGGGCAGGGCTGAGCCACAGCCCCCAGGGCCAGTCATGGGCCGAGGCCAGGGA TGCCCACCTGCCTCACCGGCTGCCGTACCTATGCTGTCCCAGGAGCTGCGCCCACCGTCAGTCGTCCAGC CCCAGCCCCTGGTGGTAGTGAAGGAGGAGAAGATTCACTCACCAATCATCCGCAGTGAGCCTTTTAGCAC CTCACTTCGCCCAGAGCCGCCCAAGCACCCAGAGAACATGAAGGCCCCAGTCCACCTGCCTCAGCGGCCT GAGATGAAGCCTGTAGATGTAGGGAGGCCTGTGATCCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGG CCCCTGACAAGGACAAACAGAAACAGGAGCCAAAAACACCAGTGGCGCCCAAAAAGGACCTGAAAATTAA GAACATGGGCTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCACCCCATCCTCCACAGCCAAGTCCTCA AGCGACAGCTTTGAGCATTTCCGCCGCGCTGCTCGGGAGAAGGAGGAGCGGGAAAAGGCCCTGAAGGCTC AGGCTGAGCACGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAGCCGAGAGGATGAGGA TGCGCTGGAACAGGCACGTCGAGCCCATGAGGAGGCACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAG AGACAGGAGCAGCAGCAGCAGCAGCAACAGCAGGCAGCTGCTGTGGCCGCTGCCTCCGCCCCCCAGGCCC AGAGCTCCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGTTGGCCCGAAAGCGAGAACAGGAGCGGAG GCGCAGGGAGGCAATGGCAGCTACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAA AATCTTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208565 representing NM_001100903 Red=Cloning site Green=Tags(s) MSTESGPGTRLRNLPVMGDGLETSQMSTTQAQAQPQSANAASTNPPPPETSNPNKPKRQTNQLQYLLRVV LKTLWKHQFAWPFQQPVDAVKLNLPDYYKIIKTPMDMGTIKKRLENNYYWNAQECIQDFNTMFTNCYIYN KPGDDIVLMAEALEKLFLQKINELPTEETEIMIVQAKGRGRGRKETGTAKPGVSTVPNTTQASTSPQTQM PQQNPPPPVQATTHPFPAVTPDLIAQPPVMTMVPPQPLQTPSPVPPQPPPPPAPVPQPVQSHPPIIATTP QPVKTKKGVKRKADTTTPTTIDPIHEPPSLAPEPKTAKLGPRRESSRPVKPPKKDVPDSQQHPGPEKSSK ISEQLKCCSGILKEMFAKKHAAYAWPFYKPVDVEALGLHDYCDIIKHPMDMSTIKSKLESREYRDAQEFG ADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPEEPVVTVSSPAVPPPTKVVAPPSSSDSS SDSSSDSDSSTDDSEEERAQRLAELQEQLKAVHEQLAALSQPQQNKPKKKEKDKKEKKKEKHKKKEEMEE NKKSKAKELPPKKTKKNNSSNSNVSKKEPAPTKTKPPPTYESEEEDKCKPMSYEEKRQLSLDINKLPGEK LGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQAEKVDVIAGSSKMKGFS SSESESTSESSSSDSEDSETEMAPKSKKKGHTGRDQKKHHHHHHPQMQPAPAPVPQQPPPPPQQPPPPPP PQQQQQQQQQQPPPPPPPPSMPQQTAPAMKSSPPPFITAQVPVLEPQLPGSVFDPIGHFTQPILHLPQPE LPPHLPQPPEHSTPPHLNQHAVVSPPALHNALPQQPSRPSNRAAALPPKPARPPAVSPALAQPPLLPQPP MAQPPQVLLEDEEPPAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQQLQQQ PPPPPPPQPQPPPQQQHQPPPRPVHLPSMPFSAHIQQPPPPTGQQPTHPPPGQQPPPPQPAKPQQVIQHH PSPRHHKSDPYSAGHLREAPSPLMIHSPQMPQFQSLTHQSPPQQNVQPKKQVKGRAEPQPPGPVMGRGQG CPPASPAAVPMLSQELRPPSVVQPQPLVVVKEEKIHSPIIRSEPFSTSLRPEPPKHPENMKAPVHLPQRP EMKPVDVGRPVIRPPEQSAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTPSSTAKSS SDSFEHFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQARRAHEEARRRQEQQQQQQ RQEQQQQQQQQAAAVAAASAPQAQSSQPQSMLDQQRELARKREQERRRREAMAATIDMNFQSDLLSIFEE NLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100903
ORF Size	4209 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100903.1, NP_001094373.1
RefSeq Size	7037 bp
RefSeq ORF	4212 bp
Locus ID	362844
MW	156.3 kDa

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