Brd4 (NM_001100903) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208565 representing NM_001100903 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACGGAGAGCGGCCCTGGGACAAGATTGAGAAATCTGCCAGTAATGGGGGATGGACTAGAAACCT CCCAAATGTCTACAACGCAGGCCCAGGCCCAACCCCAGTCAGCAAATGCAGCCAGCACCAATCCTCCACC CCCAGAGACCTCCAACCCTAACAAGCCCAAGAGGCAGACCAACCAACTGCAATATCTGCTCAGAGTGGTG CTCAAGACACTATGGAAACACCAGTTTGCGTGGCCTTTCCAGCAGCCTGTGGATGCCGTCAAGCTGAACC TCCCTGATTACTATAAGATTATTAAAACACCCATGGATATGGGAACAATAAAGAAGCGCTTGGAAAACAA CTATTACTGGAATGCTCAGGAATGTATCCAGGACTTCAACACTATGTTTACAAATTGTTACATCTATAAC AAGCCTGGAGATGACATCGTCTTAATGGCAGAAGCTCTGGAGAAGCTCTTCTTGCAGAAAATCAATGAAC TGCCTACAGAAGAAACCGAGATCATGATAGTCCAGGCAAAGGGACGAGGACGAGGGAGGAAAGAAACAGG GACAGCAAAGCCTGGTGTATCCACGGTACCAAACACAACTCAAGCATCAACTTCTCCGCAGACCCAGATG CCTCAGCAGAACCCTCCTCCACCTGTGCAGGCCACAACTCACCCCTTTCCTGCTGTCACCCCAGACCTCA TTGCCCAGCCTCCTGTCATGACAATGGTGCCCCCCCAGCCACTTCAGACTCCTTCACCGGTACCCCCTCA GCCACCACCCCCACCTGCTCCAGTTCCACAGCCTGTGCAGAGTCACCCTCCCATCATTGCGACCACCCCA CAGCCTGTGAAGACAAAGAAAGGGGTGAAGAGGAAAGCAGATACCACCACCCCCACCACCATCGACCCCA TTCATGAGCCGCCCTCACTGGCCCCGGAGCCCAAGACCGCCAAGCTGGGTCCTCGGCGGGAGAGCAGCAG ACCTGTGAAGCCCCCAAAGAAGGATGTACCAGACTCCCAGCAGCACCCAGGGCCAGAGAAGAGCAGCAAG ATCTCTGAGCAGCTGAAGTGCTGCAGTGGCATCCTCAAGGAGATGTTTGCTAAGAAACATGCTGCCTATG CCTGGCCTTTCTACAAGCCCGTGGATGTGGAGGCACTGGGTCTGCACGACTACTGTGACATCATTAAACA CCCCATGGACATGAGCACAATCAAGTCTAAACTAGAGTCCCGGGAGTACAGAGATGCCCAGGAGTTTGGT GCTGATGTCCGATTGATGTTTTCCAACTGCTACAAGTACAATCCCCCTGACCATGAAGTGGTAGCCATGG CTCGAAAACTCCAGGATGTGTTTGAAATGCGCTTTGCCAAGATGCCTGATGAGCCTGAAGAGCCAGTTGT TACGGTGTCCTCCCCTGCAGTGCCACCCCCTACAAAGGTGGTAGCCCCACCCTCATCTAGTGACAGCAGC AGTGATAGTTCCTCTGACAGTGACAGTTCCACTGACGACTCTGAGGAAGAGCGAGCCCAGCGGCTGGCTG AGCTCCAGGAGCAGCTCAAAGCCGTGCATGAGCAGCTTGCAGCCCTCTCACAGCCCCAACAGAACAAACC AAAGAAAAAGGAGAAGGACAAGAAGGAAAAGAAAAAGGAAAAGCACAAAAAGAAAGAAGAAATGGAGGAA AATAAAAAAAGCAAAGCCAAGGAACTTCCTCCCAAAAAGACAAAGAAAAATAACAGCAGCAACAGCAATG TGAGCAAGAAGGAACCAGCACCCACGAAGACCAAGCCGCCTCCCACATATGAATCAGAAGAAGAGGACAA GTGTAAGCCTATGTCTTATGAGGAGAAGCGACAGCTAAGTCTAGATATCAACAAACTTCCTGGTGAGAAG CTAGGCCGTGTAGTACACATAATTCAGTCGAGGGAACCATCACTTAAAAACTCCAACCCTGATGAGATTG AGATTGATTTTGAGACCCTGAAGCCATCTACACTACGAGAGTTGGAGCGATATGTCACCTCCTGTTTGCG GAAGAAAAGGAAACCTCAAGCTGAAAAAGTTGATGTGATTGCTGGTTCTTCCAAGATGAAGGGCTTCTCA TCCTCTGAGTCGGAGAGCACCAGCGAATCTAGCTCCTCTGACAGTGAAGACTCTGAAACAGAGATGGCTC CCAAGTCAAAAAAGAAGGGGCACACTGGGAGGGACCAGAAAAAGCATCATCACCATCACCACCCACAGAT GCAGCCAGCCCCAGCTCCTGTGCCCCAGCAGCCGCCGCCACCTCCACAGCAGCCTCCTCCACCCCCACCT CCTCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCCCCACCCCCACCACCTCCGCCCTCCATGCCACAGC AGACTGCCCCAGCGATGAAGTCCTCGCCCCCACCCTTCATCACTGCCCAGGTACCCGTCCTGGAACCACA GCTACCAGGCAGTGTCTTTGACCCTATTGGCCACTTCACCCAGCCCATCTTGCACCTGCCGCAGCCAGAG CTGCCTCCTCACCTGCCCCAGCCACCGGAGCACAGCACTCCACCCCATCTCAACCAGCATGCTGTGGTCT CTCCTCCAGCTTTGCACAATGCGCTGCCCCAACAACCATCTCGGCCCAGCAACCGAGCTGCTGCTCTGCC CCCAAAGCCTGCCCGACCCCCAGCTGTGTCCCCTGCCTTGGCCCAGCCCCCCCTGCTCCCACAGCCACCT ATGGCTCAGCCCCCCCAAGTGCTGCTGGAGGATGAAGAGCCGCCTGCCCCACCCCTCACCTCCATGCAGA TGCAGTTGTACCTACAGCAGCTGCAGAAGGTGCAGCCTCCCACACCACTACTCCCTTCCGTGAAGGTGCA GTCCCAACCCCCACCACCTTTGCCGCCTCCACCCCACCCTTCTGTGCAGCAACAGCAGCTCCAGCAGCAG CCACCACCACCCCCACCTCCTCAGCCACAGCCACCACCCCAGCAACAACACCAGCCCCCACCACGACCAG TTCACTTGCCATCCATGCCCTTCTCTGCTCACATTCAGCAGCCCCCACCACCCACAGGACAGCAACCCAC CCACCCACCCCCAGGACAGCAGCCACCCCCACCACAGCCTGCCAAGCCTCAGCAAGTCATCCAGCATCAC CCTTCACCCCGGCACCACAAGTCAGACCCCTACTCAGCTGGTCATCTTCGTGAAGCCCCTTCTCCGCTTA TGATACATTCCCCTCAAATGCCACAGTTCCAGAGCCTGACCCATCAGTCTCCACCCCAGCAAAACGTCCA GCCCAAGAAGCAGGTAAAGGGCAGGGCTGAGCCACAGCCCCCAGGGCCAGTCATGGGCCGAGGCCAGGGA TGCCCACCTGCCTCACCGGCTGCCGTACCTATGCTGTCCCAGGAGCTGCGCCCACCGTCAGTCGTCCAGC CCCAGCCCCTGGTGGTAGTGAAGGAGGAGAAGATTCACTCACCAATCATCCGCAGTGAGCCTTTTAGCAC CTCACTTCGCCCAGAGCCGCCCAAGCACCCAGAGAACATGAAGGCCCCAGTCCACCTGCCTCAGCGGCCT GAGATGAAGCCTGTAGATGTAGGGAGGCCTGTGATCCGGCCTCCAGAGCAGAGTGCACCCCCACCAGGGG CCCCTGACAAGGACAAACAGAAACAGGAGCCAAAAACACCAGTGGCGCCCAAAAAGGACCTGAAAATTAA GAACATGGGCTCCTGGGCCAGCCTGGTACAGAAGCATCCGACCACCCCATCCTCCACAGCCAAGTCCTCA AGCGACAGCTTTGAGCATTTCCGCCGCGCTGCTCGGGAGAAGGAGGAGCGGGAAAAGGCCCTGAAGGCTC AGGCTGAGCACGCAGAGAAGGAGAAGGAGCGGCTGAGGCAGGAGCGCATGAGGAGCCGAGAGGATGAGGA TGCGCTGGAACAGGCACGTCGAGCCCATGAGGAGGCACGACGGCGCCAGGAGCAGCAGCAGCAGCAGCAG AGACAGGAGCAGCAGCAGCAGCAGCAACAGCAGGCAGCTGCTGTGGCCGCTGCCTCCGCCCCCCAGGCCC AGAGCTCCCAGCCCCAGTCTATGCTGGACCAGCAGAGGGAGTTGGCCCGAAAGCGAGAACAGGAGCGGAG GCGCAGGGAGGCAATGGCAGCTACAATTGACATGAATTTCCAGAGTGATCTTTTGTCAATATTTGAAGAA AATCTTTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100903
Insert Size	4212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100903.1, NP_001094373.1
RefSeq Size	7037 bp
RefSeq ORF	4212 bp
Locus ID	362844

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