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Pfkm (NM_031715) Rat Tagged ORF Clone
CAT＃: RR208472
- TrueORF®
Pfkm (Myc-DDK-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug)
"NM_031715" in other vectors (3)
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CNY 10,070.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ATP-PFK; PFK-A; Pfk-M |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208472 representing NM_031715
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCATGAAGAGCATCATGAAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG GTGGAGATGCCCAAGGTATGAATGCTACGGTCAGGGCTGTGGTACGAGTTGGCATCTTCACCGGGCTCCG CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGATGGTGGAGAGCACATCAGGGAGGCCACCTGG GAGAGCGTGTCCATGATGCTCCAGCTGGGAGGCACGGTGATTGGAAGTGCCCGATGCAAGGACTTCCGGG AGCGAGAAGGACGACTCCGAGCTGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG AGGCGATGGCAGCCTCACTGGGGCTGACACTTTTCGTTCAGAGTGGAGTGACTTATTGAATGACCTCCAG AAAGATGGGAAGATCACAGCCGAGGAGCGTACAAAGTCCAGCTACCTGAACATCGTTTTCCTGGTTGGCT CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACTGACTCTGCCCTGCACCGCATTGTAGA GATCGTGGACGCCATCACCACCACCGCTCAGAGCCACCAGAGGACATTTGTGTTAGAAGTGATGGGCCGC CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCGTGTGGGGCCGACTGGGTTTTCATTCCCGAGTGTC CGCCAGATGACGACTGGGAAGAACACCTTTGTCGCCGTCTCAGTGAGACAAGAACCCGTGGTTCTCGTCT CAACATCATCATTGTTGCTGAGGGTGCAATCGACAAAAACGGGAAGCCAATCACCTCAGAAGACATCAAG AACCTGGTGGTAAAGCGTCTTGGATATGATACCAGGGTCACTGTTCTGGGACATGTACAGAGGGGTGGGA CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAATACGGCTGTGCGCCTGCCCCTCATGGAG TGTGTCCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA GGGCGGGTTGCACACGGTGGCTGTGATGAACGTGGGGGCCCCAGCTGCTGGAATGAATGCTGCAGTTCGC TCTACTGTGAGGATTGGCCTTATCCAAGGCAACCGAGTGCTGGTCGTGCATGATGGCTTTGAGGGTCTGG CCAAAGGTCAGATTGAGGAAGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT TGGTACTAAAAGGACTCTACCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTATAACATC CAGGGCCTGGTTATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAGGGCAGGAAGC AGTTTGATGAGCTCTGCATCCCATTTGTGGTCATTCCCGCCACGGTTTCCAATAATGTCCCAGGGTCAGA CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACCTGTGACCGAATCAAGCAGTCTGCA GCAGGCACCAAGCGGCGAGTGTTTATCATCGAGACGATGGGTGGTTACTGTGGCTATCTGGCCACCATGG CAGGACTGGCGGCTGGGGCTGATGCTGCCTACATTTTTGAGGAGCCCTTCACCATCCGAGATCTCCAGGT TAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAG TGCAACGAGAACTACACTACTGATTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTCGACA GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAACCCAACTCCCTTTGACAGGAATTTTGCCAC CAAGATGGGTGCTAAGGCTACGAATTGGATGTCTGGGAAAATCAAAGAGAGTTACCGTAATGGACGGATC TTTGCCAACACTCCCGACTCAGGTTGTGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTGA CTGAGCTGAAGGACCAGACAGATTTTGAGCACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC AATCCTCAAAATCCTGGCCAAGTACGAGATTGATCTGGACACCTCTGACCACGCCCACCTGGAGCACATT TCCAGGAAGCGGTCTGGAGAAGCTGCTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208472 representing NM_031715
Red=Cloning site Green=Tags(s) MTHEEHHEAKTLGIGKAIAVLTSGGDAQGMNATVRAVVRVGIFTGLRVFFVHEGYQGLVDGGEHIREATW ESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDLQ KDGKITAEERTKSSYLNIVFLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGR HCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIK NLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNTAVRLPLME CVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVR STVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKYNI QGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSA AGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEK CNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGNPTPFDRNFATKMGAKATNWMSGKIKESYRNGRI FANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHI SRKRSGEAAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031715 |
| ORF Size | 2340 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031715.1, NP_113903.1 |
| RefSeq Size | 2757 bp |
| RefSeq ORF | 2343 bp |
| Locus ID | 65152 |
| UniProt ID | P47858 |
| MW | 85.6 kDa |
| Gene Summary | phosphofructokinase isozyme A; may regulate glycolytic flux and influence insulin secretion [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208472 | Pfkm (untagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 10,070.00 |
|
| RR208472L3 | Lenti ORF clone of Pfkm (Myc-DDK-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 11,880.00 |
|
| RR208472L4 | Lenti ORF clone of Pfkm (mGFP-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 13,020.00 |
