Pfkm (NM_031715) Rat Untagged Clone
CAT#: RN208472
Pfkm (untagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug)
CNY 10,070.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | ATP-PFK; PFK-A; Pfk-M |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN208472 representing NM_031715
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCATGAAGAGCATCATGAAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG GTGGAGATGCCCAAGGTATGAATGCTACGGTCAGGGCTGTGGTACGAGTTGGCATCTTCACCGGGCTCCG CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGATGGTGGAGAGCACATCAGGGAGGCCACCTGG GAGAGCGTGTCCATGATGCTCCAGCTGGGAGGCACGGTGATTGGAAGTGCCCGATGCAAGGACTTCCGGG AGCGAGAAGGACGACTCCGAGCTGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG AGGCGATGGCAGCCTCACTGGGGCTGACACTTTTCGTTCAGAGTGGAGTGACTTATTGAATGACCTCCAG AAAGATGGGAAGATCACAGCCGAGGAGCGTACAAAGTCCAGCTACCTGAACATCGTTTTCCTGGTTGGCT CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACTGACTCTGCCCTGCACCGCATTGTAGA GATCGTGGACGCCATCACCACCACCGCTCAGAGCCACCAGAGGACATTTGTGTTAGAAGTGATGGGCCGC CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCGTGTGGGGCCGACTGGGTTTTCATTCCCGAGTGTC CGCCAGATGACGACTGGGAAGAACACCTTTGTCGCCGTCTCAGTGAGACAAGAACCCGTGGTTCTCGTCT CAACATCATCATTGTTGCTGAGGGTGCAATCGACAAAAACGGGAAGCCAATCACCTCAGAAGACATCAAG AACCTGGTGGTAAAGCGTCTTGGATATGATACCAGGGTCACTGTTCTGGGACATGTACAGAGGGGTGGGA CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAATACGGCTGTGCGCCTGCCCCTCATGGAG TGTGTCCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA GGGCGGGTTGCACACGGTGGCTGTGATGAACGTGGGGGCCCCAGCTGCTGGAATGAATGCTGCAGTTCGC TCTACTGTGAGGATTGGCCTTATCCAAGGCAACCGAGTGCTGGTCGTGCATGATGGCTTTGAGGGTCTGG CCAAAGGTCAGATTGAGGAAGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT TGGTACTAAAAGGACTCTACCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTATAACATC CAGGGCCTGGTTATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAGGGCAGGAAGC AGTTTGATGAGCTCTGCATCCCATTTGTGGTCATTCCCGCCACGGTTTCCAATAATGTCCCAGGGTCAGA CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACCTGTGACCGAATCAAGCAGTCTGCA GCAGGCACCAAGCGGCGAGTGTTTATCATCGAGACGATGGGTGGTTACTGTGGCTATCTGGCCACCATGG CAGGACTGGCGGCTGGGGCTGATGCTGCCTACATTTTTGAGGAGCCCTTCACCATCCGAGATCTCCAGGT TAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAG TGCAACGAGAACTACACTACTGATTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTCGACA GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAACCCAACTCCCTTTGACAGGAATTTTGCCAC CAAGATGGGTGCTAAGGCTACGAATTGGATGTCTGGGAAAATCAAAGAGAGTTACCGTAATGGACGGATC TTTGCCAACACTCCCGACTCAGGTTGTGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTGA CTGAGCTGAAGGACCAGACAGATTTTGAGCACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC AATCCTCAAAATCCTGGCCAAGTACGAGATTGATCTGGACACCTCTGACCACGCCCACCTGGAGCACATT TCCAGGAAGCGGTCTGGAGAAGCTGCTGTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_031715 |
Insert Size | 2343 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031715.1, NP_113903.1 |
RefSeq Size | 2757 bp |
RefSeq ORF | 2343 bp |
Locus ID | 65152 |
UniProt ID | P47858 |
Gene Summary | phosphofructokinase isozyme A; may regulate glycolytic flux and influence insulin secretion [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR208472 | Pfkm (Myc-DDK-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 10,070.00 |
|
RR208472L3 | Lenti ORF clone of Pfkm (Myc-DDK-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 11,880.00 |
|
RR208472L4 | Lenti ORF clone of Pfkm (mGFP-tagged ORF) - Rat phosphofructokinase, muscle (Pfkm), (10 ug) |
CNY 13,020.00 |