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Pif1 (NM_001044253) Rat Tagged ORF Clone
CAT＃: RR207530
- TrueORF®
Pif1 (Myc-DDK-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug)
"NM_001044253" in other vectors (3)
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CNY 8,270.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207530 representing NM_001044253
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCTAGCACTGAGGTGGCGACCGATGAATGTGATGACACCGAGCTGCGGTGCCGGGTAGCCGTAG AGGAGCTGAGTCCTGGAGGGCAACCTCGCAAGCGCCAGGCTCTGCGCGCCGCAGAGCTGAGCCTAGGTCG GAATGAACGCCGCGAGTTGATGCTGCGACTGCAGGCACCCGGACCCGAAGGGCGGCCACGCTGTTTCCCA CTGCGCGCCGTGCGCCTCTTCACCCGCTTCGCCGCAGTTGGGCGCAGCACTTTGCGACTCCCGGCCGATG GCGTGCCCCGGGCTGGCTCAGTGCAACTGCTGCTCTCTGACTGTCCCCCGGAGCGCTTGCGCCGCTTCCT GCGCACCCTGCGCCTGAAGCTGGCGGTCGCCCCTGGGCCGGGACCCGCCTCTGCCCGTGCGCAATTGCTC GGTCCGCGGCCCCGAGACTTTGTCACGATCAGTCCAGTGCAGCCGGAGGAACTGCGCCGTGCTGCGGCCA CCAAGGCTCCAGACTCAGCGCTGGAAAAGCGGCCAATGGAATCCCAGCCTAGTATGGAAGCTCCAAGGTG GCCCCTGCCTGTGAAGAAGCTACGCATGCCCTCCAGCAAACCGAAGCTTTCCGAAGAGCAGGCCGCTGTG CTGCGGATGGTTCTGAAAGGCCAGAGCATATTCTTTACCGGGAGTGCAGGGACAGGGAAGTCCTACCTGC TGAAACATATCCTGGGTTCCCTGCCTCCTACTGGCACTGTGGCCACTGCCAGCACTGGGGTGGCAGCCTG CCACATTGGGGGCACCACCCTTCATGCCTTTGCAGGCATCGGTTCAGGCCAGGCTCCCCTGGCCCAGTGT GTGGCTCTGGCCCATCGGCCTGGTGTGCGGCAGGGCTGGCTGAACTGCCAGCGTTTGGTCATTGATGAGA TCTCCATGGTAGAGGCAGACTTCTTTGACAAGTTGGAAGCTGTGGCCAGAGCTGTCCGGCAACAGAAGAA ACCATTTGGAGGGATCCAGCTTATTATCTGTGGGGACTTCCTACAGTTGCCGCCAGTGACCAAAGGTTCC CAGCATCCTCGATTCTGCTTTCAGGCCAAGAGCTGGAGGAAGTGTGTACCAGTGACTCTGGAGCTGACTG AGGTGTGGAGGCAAGCAGACCAGACCTTCATCTCTCTCCTGAAGGCTGTGAGATTAGGCAGATGTTCAGA TGAAGTGACCCGCCAGCTCAGGGCCACAGCTGCCCATAAGGTGGGACGAGATGGAATTATAGCCACGAGA CTGTGCACCCATCAGGATGATGTGGCCCTTACCAATGAGAAGCGGCTGAAGGAACTACCAGGTGATGTAC ACAGCTTTGAGGCTATAGACAGTGACCCTGAGCTAAGCCGGACCCTGGATGCTCAGTGCCCTGTAGGCCG TGTCCTTCAGTTAAAGCTGGGGGCTCAAGTCATGCTGGTGAAGAACTTAGCAGTGTCTCGGGGCCTGGTG AATGGTGCCCGAGGGGTGGTAGTCGGGTTTGAGTCAGAAGGAAGAGGGCTTCCCCGGGTACGGTTCCTGT GTGGCATCACTGAGGTCATCCGCACCGACCGCTGGACAGTACAGGTCACTGGGGGCCAGTACCTCAGCCG GCAGCAGCTTCCCCTACAGCTAGCCTGGGCAATGTCCATCCACAAAAGCCAGGGCATGTCTCTGGACTGT GTAGAGATCTCTCTGGGCCGTGTGTTCGCCAGTGGTCAAGCCTATGTGGCCCTCTCCCGGGCCCGTAGCC TCCAGGGTCTACGTGTGCTAGACTTCGACCCCACTGTGGTTCGATGCGACTCCCGAGTGCTGCAATTCTA TGCCACCCTGCGGCAGGGCAGGGGCCTCAGTCTGGAGTCGCAAGACGATGAGGAGGCAAGCTCAGATCTG GAGAACATGGACCCAAACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207530 representing NM_001044253
Red=Cloning site Green=Tags(s) MPSSTEVATDECDDTELRCRVAVEELSPGGQPRKRQALRAAELSLGRNERRELMLRLQAPGPEGRPRCFP LRAVRLFTRFAAVGRSTLRLPADGVPRAGSVQLLLSDCPPERLRRFLRTLRLKLAVAPGPGPASARAQLL GPRPRDFVTISPVQPEELRRAAATKAPDSALEKRPMESQPSMEAPRWPLPVKKLRMPSSKPKLSEEQAAV LRMVLKGQSIFFTGSAGTGKSYLLKHILGSLPPTGTVATASTGVAACHIGGTTLHAFAGIGSGQAPLAQC VALAHRPGVRQGWLNCQRLVIDEISMVEADFFDKLEAVARAVRQQKKPFGGIQLIICGDFLQLPPVTKGS QHPRFCFQAKSWRKCVPVTLELTEVWRQADQTFISLLKAVRLGRCSDEVTRQLRATAAHKVGRDGIIATR LCTHQDDVALTNEKRLKELPGDVHSFEAIDSDPELSRTLDAQCPVGRVLQLKLGAQVMLVKNLAVSRGLV NGARGVVVGFESEGRGLPRVRFLCGITEVIRTDRWTVQVTGGQYLSRQQLPLQLAWAMSIHKSQGMSLDC VEISLGRVFASGQAYVALSRARSLQGLRVLDFDPTVVRCDSRVLQFYATLRQGRGLSLESQDDEEASSDL ENMDPNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001044253 |
| ORF Size | 1911 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001044253.1, NP_001037718.1 |
| RefSeq Size | 1915 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 367645 |
| UniProt ID | Q1HG60 |
| MW | 69.8 kDa |
| Gene Summary | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207530 | Pif1 (untagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 8,270.00 |
|
| RR207530L3 | Lenti ORF clone of Pif1 (Myc-DDK-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 9,980.00 |
|
| RR207530L4 | Lenti ORF clone of Pif1 (mGFP-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 10,930.00 |
