Ngly1 (NM_001014136) Rat Tagged ORF Clone
CAT#: RR207395
- TrueORF®
Ngly1 (Myc-DDK-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug)
"NM_001014136" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 7,790.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR207395 representing NM_001014136
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCTCCGGCCGTGGCCGAGTTATGCCAGAACA CCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAAACCCCAG TGATGAAAAATACAGATCCATCCGCATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA GCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACACCTTATCTTTCCTAAAAAAGCTT CAGTGGAACAGCTTCAGAAAATCCGTGACCTGATTGCTGTAGAGAGAAGGAGCAGACTGGATGGATCAAG TCAGAAGGTGGAGTTCTCTCAGCACCCTGCTGCTGTCAGGCTTCCTGCCGAGCAGCCTGAGGATCCTACA GGATTAATGCAGCACTCAGGAAACCAACCTGGGCAGCCGCTGAGCCTACCCTCTGCTCCACTGGTTGTTG GTGATTCAACCATCTTTAAAGTTCTTCAGTCCAACATTCAGCACGTGCAGCTCTATGAAAATCCTGTCCT CCAGGAGAAAGCCTTGGCTTGTATCCCAGTCAATGAACTCAAAAGAAAATCTCAAGAAAAACTATTCAGA GCTAGAAAGTTAGATAAAGGTACTAAAGTAAGTGATGAGGATTTCCTTCTACTGGAGCTCCTACACTGGT TTAAAGAGGAGTTTTTCCACTGGGTGAATAACGTTGTGTGCAGCAGATGTGGCAGAGAGACTAGATCTAG AGATGAGGCCTTGCCGCCCAATGATGATGAGCTGAAGTGGGGCGCCAAGAATGTGGAGGATCATTACTGT GATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCAGAGAAACTTTTGGAAACAAGATGTG GACGCTGTGGTGAATGGGCCAATTGTTTTACGCTATGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGT GTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTGCATTGTGAT GCATGTGAGGATGTCTGCGACAAACCTCTTCTTTATGAAATAGGATGGGGAAAGAAGCTCTCCTATATCA TAGCATTCTCTAAAGATGAGGTGGTTGACGTCACCTGGAGATACTCTTGTAAACATGAAGAGGTGATGTC CAGGAGAACCAAGGTCAAAGAAGAGTTACTTAGAGAAACTATCAATGGGCTTAATAAGCAGAGACAGCTA TTATTATCAGAAAGCAGAAGAAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTC CCAAAACCCCCCGACCTGGAGAACTTGGTGGAAGGGTATCTGGGTCATTGGCTTGGAGAGTGGCCCGAGG TGAGACGTGTCTGGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAAGCAATTC CACCTCCGTTATGATATTGTGAGAGATCGTTACATCCGTGTCTCAGACAACAATGCAAACATTTCTGGAT GGGAGAACGGTGTGTGGAAAATGGAATCCATATTCAGAAAGGTTGAGAAAGACTGGAACATGGTTTATTT GGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGGTCTAAAG GTGGATAACGTTTCCATCCGGACGAGTAGCCAAAGCTTTGAGACTGGATCAGTGAGGTGGAAACTTCGCT CTGAGATGGCTCAAGTCAACCTGCTGGGAGGTAGGTCCAGACAGAAATCTGCGTTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207395 representing NM_001014136
Red=Cloning site Green=Tags(s) MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRG AVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAVERRSRLDGSSQKVEFSQHPAAVRLPAEQPEDPT GLMQHSGNQPGQPLSLPSAPLVVGDSTIFKVLQSNIQHVQLYENPVLQEKALACIPVNELKRKSQEKLFR ARKLDKGTKVSDEDFLLLELLHWFKEEFFHWVNNVVCSRCGRETRSRDEALPPNDDELKWGAKNVEDHYC DACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCD ACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHEEVMSRRTKVKEELLRETINGLNKQRQL LLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETCLERKEILFIPSENEKISKQF HLRYDIVRDRYIRVSDNNANISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLK VDNVSIRTSSQSFETGSVRWKLRSEMAQVNLLGGRSRQKSAFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001014136 |
ORF Size | 1809 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001014136.1, NP_001014158.1 |
RefSeq Size | 2234 bp |
RefSeq ORF | 1812 bp |
Locus ID | 361014 |
UniProt ID | Q5XI55 |
MW | 69.2 kDa |
Gene Summary | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN207395 | Ngly1 (untagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 7,790.00 |
|
RR207395L3 | Lenti ORF clone of Ngly1 (Myc-DDK-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 9,600.00 |
|
RR207395L4 | Lenti ORF clone of Ngly1 (mGFP-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 10,450.00 |