United States
Germany
Japan
United Kingdom
China
Ngly1 (NM_001014136) Rat Untagged Clone
CAT＃: RN207395
Ngly1 (untagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug)
CNY 7,790.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207395 representing NM_001014136
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGCCACACTGGGCAGCTCGTCGAGCTCTGCGTCTCCGGCCGTGGCCGAGTTATGCCAGAACA CCCCGGAGACCTTTCTGGAGGCCTCCAAGCTGCTGCTCACCTACGCCGACAACATCCTGAGAAACCCCAG TGATGAAAAATACAGATCCATCCGCATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA GCTGTTGAGTGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACACCTTATCTTTCCTAAAAAAGCTT CAGTGGAACAGCTTCAGAAAATCCGTGACCTGATTGCTGTAGAGAGAAGGAGCAGACTGGATGGATCAAG TCAGAAGGTGGAGTTCTCTCAGCACCCTGCTGCTGTCAGGCTTCCTGCCGAGCAGCCTGAGGATCCTACA GGATTAATGCAGCACTCAGGAAACCAACCTGGGCAGCCGCTGAGCCTACCCTCTGCTCCACTGGTTGTTG GTGATTCAACCATCTTTAAAGTTCTTCAGTCCAACATTCAGCACGTGCAGCTCTATGAAAATCCTGTCCT CCAGGAGAAAGCCTTGGCTTGTATCCCAGTCAATGAACTCAAAAGAAAATCTCAAGAAAAACTATTCAGA GCTAGAAAGTTAGATAAAGGTACTAAAGTAAGTGATGAGGATTTCCTTCTACTGGAGCTCCTACACTGGT TTAAAGAGGAGTTTTTCCACTGGGTGAATAACGTTGTGTGCAGCAGATGTGGCAGAGAGACTAGATCTAG AGATGAGGCCTTGCCGCCCAATGATGATGAGCTGAAGTGGGGCGCCAAGAATGTGGAGGATCATTACTGT GATGCCTGCCAGTTGAGCAACCGCTTCCCAAGATATAACAACCCAGAGAAACTTTTGGAAACAAGATGTG GACGCTGTGGTGAATGGGCCAATTGTTTTACGCTATGCTGCCGTGCATTAGGGTTTGAAGCTCGATATGT GTGGGATTACACAGACCATGTTTGGACAGAAGTCTATTCTCCATCTCAGCAGCGGTGGCTGCATTGTGAT GCATGTGAGGATGTCTGCGACAAACCTCTTCTTTATGAAATAGGATGGGGAAAGAAGCTCTCCTATATCA TAGCATTCTCTAAAGATGAGGTGGTTGACGTCACCTGGAGATACTCTTGTAAACATGAAGAGGTGATGTC CAGGAGAACCAAGGTCAAAGAAGAGTTACTTAGAGAAACTATCAATGGGCTTAATAAGCAGAGACAGCTA TTATTATCAGAAAGCAGAAGAAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTC CCAAAACCCCCCGACCTGGAGAACTTGGTGGAAGGGTATCTGGGTCATTGGCTTGGAGAGTGGCCCGAGG TGAGACGTGTCTGGAGAGAAAGGAGATTCTGTTCATCCCTTCTGAAAATGAAAAGATTTCTAAGCAATTC CACCTCCGTTATGATATTGTGAGAGATCGTTACATCCGTGTCTCAGACAACAATGCAAACATTTCTGGAT GGGAGAACGGTGTGTGGAAAATGGAATCCATATTCAGAAAGGTTGAGAAAGACTGGAACATGGTTTATTT GGCCCGAAAAGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAATGTGGGTCAGCTGGTCTAAAG GTGGATAACGTTTCCATCCGGACGAGTAGCCAAAGCTTTGAGACTGGATCAGTGAGGTGGAAACTTCGCT CTGAGATGGCTCAAGTCAACCTGCTGGGAGGTAGGTCCAGACAGAAATCTGCGTTCCTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014136 |
| Insert Size | 1812 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014136.1, NP_001014158.1 |
| RefSeq Size | 2234 bp |
| RefSeq ORF | 1812 bp |
| Locus ID | 361014 |
| UniProt ID | Q5XI55 |
| Gene Summary | Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207395 | Ngly1 (Myc-DDK-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 7,790.00 |
|
| RR207395L3 | Lenti ORF clone of Ngly1 (Myc-DDK-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 9,600.00 |
|
| RR207395L4 | Lenti ORF clone of Ngly1 (mGFP-tagged ORF) - Rat N-glycanase 1 (Ngly1), (10 ug) |
CNY 10,450.00 |
