Evc2 (NM_001106012) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207102 representing NM_001106012 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGACCGGTCTCACGGGCACTGGGGGACAAGCCACGTGGGTGCTGGCTGGAAATATCCTGGCCG CGGCCTTGGTACTGGGTGTGGGTCCGTGTGCGCTTCCACCCTCCTTCCCTGCCCTGGGCCCAGGGTCGCC ATCCCCACCCGACCCTGTAGGACCCCGGGCAAGCTCACAGCACAGCGATATTTCAAGAGATGCCCAAGGT CCCTCAGAGAACGGAGTGATATTTCAAAAATGCTCGCTGGTGTCTGGGCAGAGCGAATCGCAGACCATGC ATGTGCAGCTGTCAGTGAACAACACCAGGACGCCCATGTCAGCCAACCTTTCGGACCTGCTTGTGCTGGA TGACATCACTGGCCTCACTGTGAAGGAGTCACCTGGAAATGTGACTCAGGAAGGAATCCAGACTTTTAGA AAGCGGTTTCTGCAAGTGGGAGAGTGCTACTCCGTCAGCTACATGGCCTCGCTGGACCCCACAGCCCTTG GGCCTGGAGAGAGCCTGGATCTTCCCGCCCAGCTCATCTTTCAGAGACCCTCACAGAACAGAACTCAGCT AAGAGCCCGCTTTACCATCACCGTGGAAGAAAAGATAATGGTTCTGCCTAACCATGGTCTCCACACAGCA GGCTTCATCGCAGCCTTCCTCATCTCCTTCCTCCTGACCTTGGCAGCCCTGTTCTTCCTGGCTCGGGGTC GGTGTCTGCAGGGAAGCATGCTGTTGAGATGCCGGGTTCAGCATCCTGAGAACAAGCTGGAGCCCTCACC CTTCACCTCTGCTAATGGTGTGAGCCAGGACCTTTCCCTCAATGACCAACTGGTTGCCATCCTGACTTCT GAGGAGCCTGGGAGCATGCTTCAAGCCCTAGAAGAGTTGGAGATCGCAACCCTGAATCAGGCAGATGCGG ATCTGGAGGCTTGTCGAAACCAGATTAGTAAGGACATCATTGCCCTTCTGATGAAGAATCTGATCAGTGG TGGTCACCTCTCTCTGCAAACAGAAAGGAAGATGGCTGCTGCTTTTAAAAAGCAGTTTCTGCTGCTGGAA AACGAAATCCAGGAGGAGTATGAGCGGAAGATGTTGGCACTCACGGCAGAGTGTGACCTAGAAGTGAGGA AGAAGACAGAAAACCAGTACCAGAGGGAGATGGTGGCCATGGAGGAAGCAGAAGAGGTGTTAAAGCGAGT TAGTGAGAGGTCTGCTGCAGAGTGCAGCAGCCTTTTGCGGACCCTCCATGGGCTGGAGCAGGAGGACCTG CAGAGGTCCCTCACTCTGCAGCAAGCAGAGGACTTTGCCCAAGCACACAGGCAGCTGGCTGTTTTCCAGA GAAACGAATTACACAGCATCGTCTATACCCAGATACAGAGTGCTGTTGCCAAAGGAGAACTGAGGCCCGA GGTGGCTAAAATGCTGCTGCAAGACTATTCTAAAACACAGGAGAGTATAGAAGAGCTGATGGACTTCTTC CAGGCCACCAAGCGATACCATCTCAGCAAGAGGTTTGGCCACAGAGAGTACCTGGTCCAGAGACTGCAGG CCATGGAGACCCGCGTGCAAGGGCTGCTGAGCACTGCAGCTACCCAGCTAACAAGTCTCATTCATAAACA TGAGAGGGCTGGTTACTTGGATGAAGATCAGATGGAAATGCTATTGGAGCGTGCACAGACAGAAACCTTT TCTATAAAACAGAAGCTGGACAATGACTTAAAGCAAGAAAAGAAAAGGCTCCACCAAAAATTAATAACCA AGAGACGGCGAGAACTTCTGCAAAAGCACAAGGAGCAGCAGAAAGAGCAGGTGTCCCTTGGCGAGGCCTC CAGCACTGCGGAGGATGCCATTCAGTACCTGCAGCAGTGGAGGAGCGTGATGGCCGAGCACACCACTGCC CTGGAGGAGCTGCAGGAGCGGCTGGACCAGGCTGCCCTGGATGACCTCCGGGTCCTGACTGTGTCACTGT CTGAGAAAGCCACAGAGGAGCTGAGGCGCCTGCAGAGCACGGCCATGACACAGGAGCTGCTGAAGCGCAG TGCTCCCTGGCTCTTCCTGCAGCAGATCCTAGAGGAGCATAGCCGGGAGTCTGCGGCCCGTACCGCGCAG CTGGAGGCAGAGGAGAGAGAGCGTGGCCAGGAGCTGGTGCAGGGCGTGAGACAGAGGCTACAACAGGATG CACCGGAGGCTTGCACAGAAGAGCAGGCAGAGCTGAGGCATTGGGAGCACTTGGTATTCACGAAGCTCTG CTCTGCGGCCATCTCACTGTCTGAAGAAGACCTGCTCCGGGTGAGGCAGGAGGCACAGGGCTGCTTCACC CAGCTGGACCGGAGCTTGGCCCTCCCCAGAGTCCGTGCACGGGTGCTGCAGCAGCAGATGCAGATGACCT GGCGGGAAGCAGAGTTCCGGAAACTGGACCAGGCTCTGGCTGTGCCTGAGCTGCAGTCCAAGTCAAGAAA GCTGCGTTCCAAGGGCAGAGGCAAGGCGGACCTTCTGAAGAAGAACCTGGAGGATAAGATCCGCCTGTTC GAGGAGCGGGCCCCTGAAGACCTGGCTGACCAGGTACGAGGGGAATTGCTCCAGGAGAGGGTCCAGAGGC TGGAGGCCCAGGAGGCACACTTTGCCGAGTCACTTGTGGCTCTGCAGTTCCAGAAGGTGGCCCGGGCTGC TGAGACCCTCTCCGTCTACACCGCCCTGCTTAGCATCCAAGACCTGCTGCTGGGGGAGCTGAGTGAGTCG GAGACACTGACCAAGTCAGCCTGCGTGCAGATCCTGGAGTCCCACAGGCCGGAGCTGCTGGAGCTGGAGA GGATGCTGGAGGACCGGCTGGTGCAGCAGGAAGAGGCTGAGCAGCGGCGGGTCCTGGAGAGCAGGCAGCG GTGGGCGGCTGACGGACCTGCGCTGGGCGAGCCTGAGGAGATAGATCCCGAGAGGCAGGTCTCTGCCGTC CTGCGGCAGGCTCTGAACAAGGGCCAGAAGCTACTGGAACAGTATCAGCAGAGGGTGAGAGAGGAGCAGC AGAACGGTGCAGTGCTGGAAGATTCTCTGGAAAGCATGGAGACCGACACCATGGCCAGCCTCTGCAGCCA GGGACTGAGGCTGGTGTCCTACCTCTCCAGGATGACAATGGTGCCGGGGGGCACCCTACTGAGACTCCTG AGTGTGGTGCTTCCCGCAGCTTCACAGCCTCAGCTACTGGCCTTGCTGGATGCAGTCAGCGAGAAGCACT CAGACCACACAGCAGAGAATGAGAGTGGTGGTGAGCAGGCACAGGCGGAGCAGGGCAAGAGGAGAAAACA CCAGAGCTGGTGGAAAGCTTTGGACAGCAGGTTCAGAGCAGATCTGGTGAGTCAAGGACTGGAGAGGATG CTCTGGGCCCGCCGGAAGAAGGAGAGCATATTGAAGAAGAGATATGTTCCTGTCCAGGAGAGGGTGGTGT TCCCCGGGAAAGGAAGCTGGCCGCACCTGTCCCTAGAGCCCATCGGAGAACTGGCGCCCATCCCCATCAC AGGGCTAGACACTATGGACATATTAAACACAGGAGAAAAGATCTTTGTATTCAGAAGCCCAAAGGAGCCT GAGATCTCTTTGCGTGTTCCTCCCAGAAGGAAGAAGAATTTCCTGAATGCCAAGGCAGCCAGCAGGGCCT TGGGCTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207102 representing NM_001106012 Red=Cloning site Green=Tags(s) MGATGLTGTGGQATWVLAGNILAAALVLGVGPCALPPSFPALGPGSPSPPDPVGPRASSQHSDISRDAQG PSENGVIFQKCSLVSGQSESQTMHVQLSVNNTRTPMSANLSDLLVLDDITGLTVKESPGNVTQEGIQTFR KRFLQVGECYSVSYMASLDPTALGPGESLDLPAQLIFQRPSQNRTQLRARFTITVEEKIMVLPNHGLHTA GFIAAFLISFLLTLAALFFLARGRCLQGSMLLRCRVQHPENKLEPSPFTSANGVSQDLSLNDQLVAILTS EEPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLISGGHLSLQTERKMAAAFKKQFLLLE NEIQEEYERKMLALTAECDLEVRKKTENQYQREMVAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDL QRSLTLQQAEDFAQAHRQLAVFQRNELHSIVYTQIQSAVAKGELRPEVAKMLLQDYSKTQESIEELMDFF QATKRYHLSKRFGHREYLVQRLQAMETRVQGLLSTAATQLTSLIHKHERAGYLDEDQMEMLLERAQTETF SIKQKLDNDLKQEKKRLHQKLITKRRRELLQKHKEQQKEQVSLGEASSTAEDAIQYLQQWRSVMAEHTTA LEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTAQ LEAEERERGQELVQGVRQRLQQDAPEACTEEQAELRHWEHLVFTKLCSAAISLSEEDLLRVRQEAQGCFT QLDRSLALPRVRARVLQQQMQMTWREAEFRKLDQALAVPELQSKSRKLRSKGRGKADLLKKNLEDKIRLF EERAPEDLADQVRGELLQERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLSIQDLLLGELSES ETLTKSACVQILESHRPELLELERMLEDRLVQQEEAEQRRVLESRQRWAADGPALGEPEEIDPERQVSAV LRQALNKGQKLLEQYQQRVREEQQNGAVLEDSLESMETDTMASLCSQGLRLVSYLSRMTMVPGGTLLRLL SVVLPAASQPQLLALLDAVSEKHSDHTAENESGGEQAQAEQGKRRKHQSWWKALDSRFRADLVSQGLERM LWARRKKESILKKRYVPVQERVVFPGKGSWPHLSLEPIGELAPIPITGLDTMDILNTGEKIFVFRSPKEP EISLRVPPRRKKNFLNAKAASRALGLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106012
ORF Size	3651 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106012.1, NP_001099482.1
RefSeq Size	4067 bp
RefSeq ORF	3654 bp
Locus ID	289711
MW	137.2 kDa

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