Evc2 (NM_001106012) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207102 representing NM_001106012 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCGACCGGTCTCACGGGCACTGGGGGACAAGCCACGTGGGTGCTGGCTGGAAATATCCTGGCCG CGGCCTTGGTACTGGGTGTGGGTCCGTGTGCGCTTCCACCCTCCTTCCCTGCCCTGGGCCCAGGGTCGCC ATCCCCACCCGACCCTGTAGGACCCCGGGCAAGCTCACAGCACAGCGATATTTCAAGAGATGCCCAAGGT CCCTCAGAGAACGGAGTGATATTTCAAAAATGCTCGCTGGTGTCTGGGCAGAGCGAATCGCAGACCATGC ATGTGCAGCTGTCAGTGAACAACACCAGGACGCCCATGTCAGCCAACCTTTCGGACCTGCTTGTGCTGGA TGACATCACTGGCCTCACTGTGAAGGAGTCACCTGGAAATGTGACTCAGGAAGGAATCCAGACTTTTAGA AAGCGGTTTCTGCAAGTGGGAGAGTGCTACTCCGTCAGCTACATGGCCTCGCTGGACCCCACAGCCCTTG GGCCTGGAGAGAGCCTGGATCTTCCCGCCCAGCTCATCTTTCAGAGACCCTCACAGAACAGAACTCAGCT AAGAGCCCGCTTTACCATCACCGTGGAAGAAAAGATAATGGTTCTGCCTAACCATGGTCTCCACACAGCA GGCTTCATCGCAGCCTTCCTCATCTCCTTCCTCCTGACCTTGGCAGCCCTGTTCTTCCTGGCTCGGGGTC GGTGTCTGCAGGGAAGCATGCTGTTGAGATGCCGGGTTCAGCATCCTGAGAACAAGCTGGAGCCCTCACC CTTCACCTCTGCTAATGGTGTGAGCCAGGACCTTTCCCTCAATGACCAACTGGTTGCCATCCTGACTTCT GAGGAGCCTGGGAGCATGCTTCAAGCCCTAGAAGAGTTGGAGATCGCAACCCTGAATCAGGCAGATGCGG ATCTGGAGGCTTGTCGAAACCAGATTAGTAAGGACATCATTGCCCTTCTGATGAAGAATCTGATCAGTGG TGGTCACCTCTCTCTGCAAACAGAAAGGAAGATGGCTGCTGCTTTTAAAAAGCAGTTTCTGCTGCTGGAA AACGAAATCCAGGAGGAGTATGAGCGGAAGATGTTGGCACTCACGGCAGAGTGTGACCTAGAAGTGAGGA AGAAGACAGAAAACCAGTACCAGAGGGAGATGGTGGCCATGGAGGAAGCAGAAGAGGTGTTAAAGCGAGT TAGTGAGAGGTCTGCTGCAGAGTGCAGCAGCCTTTTGCGGACCCTCCATGGGCTGGAGCAGGAGGACCTG CAGAGGTCCCTCACTCTGCAGCAAGCAGAGGACTTTGCCCAAGCACACAGGCAGCTGGCTGTTTTCCAGA GAAACGAATTACACAGCATCGTCTATACCCAGATACAGAGTGCTGTTGCCAAAGGAGAACTGAGGCCCGA GGTGGCTAAAATGCTGCTGCAAGACTATTCTAAAACACAGGAGAGTATAGAAGAGCTGATGGACTTCTTC CAGGCCACCAAGCGATACCATCTCAGCAAGAGGTTTGGCCACAGAGAGTACCTGGTCCAGAGACTGCAGG CCATGGAGACCCGCGTGCAAGGGCTGCTGAGCACTGCAGCTACCCAGCTAACAAGTCTCATTCATAAACA TGAGAGGGCTGGTTACTTGGATGAAGATCAGATGGAAATGCTATTGGAGCGTGCACAGACAGAAACCTTT TCTATAAAACAGAAGCTGGACAATGACTTAAAGCAAGAAAAGAAAAGGCTCCACCAAAAATTAATAACCA AGAGACGGCGAGAACTTCTGCAAAAGCACAAGGAGCAGCAGAAAGAGCAGGTGTCCCTTGGCGAGGCCTC CAGCACTGCGGAGGATGCCATTCAGTACCTGCAGCAGTGGAGGAGCGTGATGGCCGAGCACACCACTGCC CTGGAGGAGCTGCAGGAGCGGCTGGACCAGGCTGCCCTGGATGACCTCCGGGTCCTGACTGTGTCACTGT CTGAGAAAGCCACAGAGGAGCTGAGGCGCCTGCAGAGCACGGCCATGACACAGGAGCTGCTGAAGCGCAG TGCTCCCTGGCTCTTCCTGCAGCAGATCCTAGAGGAGCATAGCCGGGAGTCTGCGGCCCGTACCGCGCAG CTGGAGGCAGAGGAGAGAGAGCGTGGCCAGGAGCTGGTGCAGGGCGTGAGACAGAGGCTACAACAGGATG CACCGGAGGCTTGCACAGAAGAGCAGGCAGAGCTGAGGCATTGGGAGCACTTGGTATTCACGAAGCTCTG CTCTGCGGCCATCTCACTGTCTGAAGAAGACCTGCTCCGGGTGAGGCAGGAGGCACAGGGCTGCTTCACC CAGCTGGACCGGAGCTTGGCCCTCCCCAGAGTCCGTGCACGGGTGCTGCAGCAGCAGATGCAGATGACCT GGCGGGAAGCAGAGTTCCGGAAACTGGACCAGGCTCTGGCTGTGCCTGAGCTGCAGTCCAAGTCAAGAAA GCTGCGTTCCAAGGGCAGAGGCAAGGCGGACCTTCTGAAGAAGAACCTGGAGGATAAGATCCGCCTGTTC GAGGAGCGGGCCCCTGAAGACCTGGCTGACCAGGTACGAGGGGAATTGCTCCAGGAGAGGGTCCAGAGGC TGGAGGCCCAGGAGGCACACTTTGCCGAGTCACTTGTGGCTCTGCAGTTCCAGAAGGTGGCCCGGGCTGC TGAGACCCTCTCCGTCTACACCGCCCTGCTTAGCATCCAAGACCTGCTGCTGGGGGAGCTGAGTGAGTCG GAGACACTGACCAAGTCAGCCTGCGTGCAGATCCTGGAGTCCCACAGGCCGGAGCTGCTGGAGCTGGAGA GGATGCTGGAGGACCGGCTGGTGCAGCAGGAAGAGGCTGAGCAGCGGCGGGTCCTGGAGAGCAGGCAGCG GTGGGCGGCTGACGGACCTGCGCTGGGCGAGCCTGAGGAGATAGATCCCGAGAGGCAGGTCTCTGCCGTC CTGCGGCAGGCTCTGAACAAGGGCCAGAAGCTACTGGAACAGTATCAGCAGAGGGTGAGAGAGGAGCAGC AGAACGGTGCAGTGCTGGAAGATTCTCTGGAAAGCATGGAGACCGACACCATGGCCAGCCTCTGCAGCCA GGGACTGAGGCTGGTGTCCTACCTCTCCAGGATGACAATGGTGCCGGGGGGCACCCTACTGAGACTCCTG AGTGTGGTGCTTCCCGCAGCTTCACAGCCTCAGCTACTGGCCTTGCTGGATGCAGTCAGCGAGAAGCACT CAGACCACACAGCAGAGAATGAGAGTGGTGGTGAGCAGGCACAGGCGGAGCAGGGCAAGAGGAGAAAACA CCAGAGCTGGTGGAAAGCTTTGGACAGCAGGTTCAGAGCAGATCTGGTGAGTCAAGGACTGGAGAGGATG CTCTGGGCCCGCCGGAAGAAGGAGAGCATATTGAAGAAGAGATATGTTCCTGTCCAGGAGAGGGTGGTGT TCCCCGGGAAAGGAAGCTGGCCGCACCTGTCCCTAGAGCCCATCGGAGAACTGGCGCCCATCCCCATCAC AGGGCTAGACACTATGGACATATTAAACACAGGAGAAAAGATCTTTGTATTCAGAAGCCCAAAGGAGCCT GAGATCTCTTTGCGTGTTCCTCCCAGAAGGAAGAAGAATTTCCTGAATGCCAAGGCAGCCAGCAGGGCCT TGGGCTTGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106012
Insert Size	3654 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106012.1, NP_001099482.1
RefSeq Size	4067 bp
RefSeq ORF	3654 bp
Locus ID	289711

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