Glg1 (NM_017211) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MG-160; Selel
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206288 representing NM_017211 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGTGTGGACGTGTACGGAGGATGTTCCGCTTGTCGGCGGCGTTGCAGCTGCTGCTGCTAGTGG CAGCAGGGGTCCAGAATAGCCACGGTCAGGGCCAGGGTCTGGGGGTCAACTTTGGGCCCTTCGCAGGGCA AGCTGGAGGCGGCAACCCGGTCGGGCAGCAGCCACCTCAGCTGCCTCAGTTATCGCAGCAGCAGCAGCAG CAGCAGCCGCCGCCTCAGCAGCAGCAGCCTTTCCCGGCGGGCGGGCTCCCGGCCCGGCGGGGCGGAGCAG GGCCCGGTGGGACTGGCGGAGGCTGGAAGCTGGCGGAGGAAGAGTCCTGCAGAGAGGACGTGACCCGTGT GTGCCCCAAACACACCTGGAGCAACAACCTGGCGGTGCTCGAGTGCCTGCAGGACGTGAGGGAGCCTGAG AATGAGATTTCTTCAGACTGCAATCATTTGTTGTGGAATTACAAGCTGAACCTGACTACTGATCCCAAAT TTGAATCTGTGGCCAGGGAAGTTTGCAAGTCAACTATTTCAGAGATTAAAGAGTGTGCTGAAGAACCAGT TGGAAAAGGTTACATGGTTTCCTGCTTGGTGGATCACCGAGGCAACATCACTGAATACCAGTGTCATCAA TACATTACCAAAATGACAGCCATCATTTTTAGTGATTATCGTTTGATCTGTGGCTTCATGGATGACTGCA AAAATGACATCAACCTCTTGAAATGTGGCAGCATTCGCCTTGGGGAAAAGGATGCACATTCACAAGGTGA GGTGGTATCATGTTTGGAAAAAGGCCTGGTGAAAGAGGCAGAAGAGAAAGAGCCCAAGATTCAAGTCTCA GAACTCTGCAAGAAAGCCATTCTTAGGGTGGCTGAGCTGTCCTCGGATGACTTTCATTTAGACCGGCATT TGTATTTTGCTTGCAGAGATGATCGGGAGCGCTTTTGTGAGAATACACAAGCTGGTGAAGGAAGAGTGTA TAAATGCCTTTTTAACCATAAATTTGAAGAATCCATGAGTGAAAAGTGTAGAGAAGCACTAACTACACGG CAGAAGCTCATTGCCCAGGATTATAAAGTCAGCTATTCATTGGCCAAGTCCTGTAAGAGTGACTTGAAAA AATACCGATGTAATGTGGAAAACCTTCCTCGCTCCCGAGAGGCCAGGCTCTCCTACCTTCTGATGTGCCT GGAGTCAGCCGTGCACAGAGGGCGACAAGTGAGCAGTGAGTGCCAAGGTGAGATGCTGGATTACCGACGC ATGCTGATGGAAGACTTCTCTCTGAGCCCCGAGATCATCCTGAGCTGTCGAGGGGAGATCGAACACCACT GTTCTGGATTACATCGGAAAGGACGCACCCTCCACTGTCTGATGAAAGTAGTCCGGGGTGAAAAGGGGAG CCTTGGAATGAACTGCCAGCAGGCGCTTCAGACACTGATTCAGGAGACTGACCCTGGCGCAGACTACCGC ATTGATCGAGCTTTGAATGAAGCTTGTGAATCTGTAATACAGACAGCCTGCAAACATATACGATCCGGAG ACCCAATGATTCTTTCATGTCTGATGGAACATTTATACACAGAGAAAATGGTGGAAGACTGTGAACACCG TCTCTTAGAGCTACAGTATTTTATCTCTCGGGATTGGAAGTTGGACCCTGTTTTATACCGGAAATGCCAG GGAGATGCTTCCCGTCTTTGCCACACCCATGGTTGGAATGAGACCAGTGAACTTATGCCTCCCGGAGCTG TGTTTTCTTGCCTGTATAGACATGCCTACCGCACAGAAGAGCAAGGACGGAGGCTCTCACGAGAATGCCG AGCTGAAGTCCAGAGGATCCTACACCAGCGTGCCATGGATGTTAAGCTGGATCCTGCCCTCCAGGACAAG TGCCTGATAGATCTAGGAAAGTGGTGCAGTGAGAAGACAGAGACTGGTCAGGAGCTTGAGTGCCTTCAGG ACCATCTGGATGACTTAGCTGTGGAATGCAGAGACATAGTGGGCAACCTCACGGAGTTAGAGTCTGAGGA CATACAAATAGAAGCTTTGCTGATGAGAGCCTGTGAGCCTATAATTCACAACTTTTGCCACGATGTGGCA GACAACCAGATTGACTCTGGGGACCTAATGGAGTGTCTGATCCAGAACAAGCACCAGAAGGACATGAATG AGAAGTGCGCCATTGGCGTCACTCACTTCCAGCTGGTCCAAATGAAGGATTTTCGATTCTCTTACAAGTT CAAAATGGCCTGCAAGGAGGATGTGTTAAAACTTTGCCCCAACATAAAAAAGAAGGTGGATGTGGTGATC TGCCTGAGCACCACCGTGCGCAACGACACTCTGCAGGAGGCCAAGGAGCACCGGGTGTCTCTCAAGTGCC GCAAGCAGCTCCGTGTGGAGGAGCTGGAGATGACAGAGGACATCCGTTTGGAACCAGATCTGTATGAAGC CTGCAAGAGTGACATCAAGAACTACTGTTCTACAGTGCAATATGGAAATGCTCAGATTATTGAATGTCTA AAAGAAAACAAGAAGCAACTGAGTACCCGTTGTCACCAGAGAGTATTTAAGCTGCAGGAGACAGAGATGA TGGACCCAGAACTAGACTATACACTCATGAGAGTCTGCAAGCAGATGATTAAGCGGTTCTGTCCAGAAGC AGATTCTAAAACTATGTTACAGTGTTTGAAACAAAACAAGAACAGTGAATTGATGGACCCCAAGTGCAAA CAGATGATCACCAAGCGTCAGATCACCCAGAACACAGATTACCGTTTAAACCCCGTGCTAAGGAAGGCCT GTAAAGCTGACATTCCTAAATTCTGTCATGGCATCCTGACCAAGGCCAAGGATGATTCAGAGCTAGAAGG CCAAGTTATCTCATGCCTCAAGCTGAGATACGCTGACCAGCGCCTGTCTTCAGACTGTGAGGATCAGATC CGTATCATCACCCAAGAGTCTGCGCTAGATTACCGCCTAGACCCTCAGCTCCAGCTGCACTGCTCAGACG AGATTGCTAATTTATGTGCTGAAGAAGCAGCAGCCCAGGAACAGACAGGCCAAGTGGAAGAGTGCCTAAA GGTCAACCTGCTCAAGATCAGGACAGAGCTGTGTAAAAAGGAAGTGTTGAACATGTTGAAGGAAAGCAAA GCAGACATCTTTGTGGATCCAGTTCTTCACACAGCGTGTGCCTTGGACATTAAACACCACTGTGCAGCCA TCACCCCCGGCCGTGGGCGTCAGATGTCCTGCCTGATGGAGGCCTTGGAAGACAAGCGCGTGAGGTTGCA GCCCGAGTGCAAAAAGCGCCTGAATGACCGAATCGAGATGTGGAGTTATGCAGCCAAGGTGGCCCCAGCG GACGGCTTCTCTGATCTTGCCATGCAAGTGATGACATCCCCATCAAAGAATTACATCCTGTCTGTGATCA GCGGGAGCATCTGCATATTGTTCCTGATTGGCCTGATGTGTGGACGGATCACTAAGAGGGTGACACGAGA GCTGAAGGACAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206288 representing NM_017211 Red=Cloning site Green=Tags(s) MAVCGRVRRMFRLSAALQLLLLVAAGVQNSHGQGQGLGVNFGPFAGQAGGGNPVGQQPPQLPQLSQQQQQ QQPPPQQQQPFPAGGLPARRGGAGPGGTGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPE NEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTISEIKECAEEPVGKGYMVSCLVDHRGNITEYQCHQ YITKMTAIIFSDYRLICGFMDDCKNDINLLKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEKEPKIQVS ELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTR QKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRR MLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGSLGMNCQQALQTLIQETDPGADYR IDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQ GDASRLCHTHGWNETSELMPPGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDK CLIDLGKWCSEKTETGQELECLQDHLDDLAVECRDIVGNLTELESEDIQIEALLMRACEPIIHNFCHDVA DNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVI CLSTTVRNDTLQEAKEHRVSLKCRKQLRVEELEMTEDIRLEPDLYEACKSDIKNYCSTVQYGNAQIIECL KENKKQLSTRCHQRVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCK QMITKRQITQNTDYRLNPVLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQI RIITQESALDYRLDPQLQLHCSDEIANLCAEEAAAQEQTGQVEECLKVNLLKIRTELCKKEVLNMLKESK ADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPA DGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017211
ORF Size	3513 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017211.2, NP_058907.1
RefSeq Size	5292 bp
RefSeq ORF	3516 bp
Locus ID	29476
UniProt ID	Q62638
MW	133.6 kDa
Gene Summary	a Golgi resident sialoglycoprotein that binds basic fibroblast growth factor [RGD, Feb 2006]

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