Glg1 (NM_017211) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	MG-160; Selel
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206288 representing NM_017211 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGTGTGGACGTGTACGGAGGATGTTCCGCTTGTCGGCGGCGTTGCAGCTGCTGCTGCTAGTGG CAGCAGGGGTCCAGAATAGCCACGGTCAGGGCCAGGGTCTGGGGGTCAACTTTGGGCCCTTCGCAGGGCA AGCTGGAGGCGGCAACCCGGTCGGGCAGCAGCCACCTCAGCTGCCTCAGTTATCGCAGCAGCAGCAGCAG CAGCAGCCGCCGCCTCAGCAGCAGCAGCCTTTCCCGGCGGGCGGGCTCCCGGCCCGGCGGGGCGGAGCAG GGCCCGGTGGGACTGGCGGAGGCTGGAAGCTGGCGGAGGAAGAGTCCTGCAGAGAGGACGTGACCCGTGT GTGCCCCAAACACACCTGGAGCAACAACCTGGCGGTGCTCGAGTGCCTGCAGGACGTGAGGGAGCCTGAG AATGAGATTTCTTCAGACTGCAATCATTTGTTGTGGAATTACAAGCTGAACCTGACTACTGATCCCAAAT TTGAATCTGTGGCCAGGGAAGTTTGCAAGTCAACTATTTCAGAGATTAAAGAGTGTGCTGAAGAACCAGT TGGAAAAGGTTACATGGTTTCCTGCTTGGTGGATCACCGAGGCAACATCACTGAATACCAGTGTCATCAA TACATTACCAAAATGACAGCCATCATTTTTAGTGATTATCGTTTGATCTGTGGCTTCATGGATGACTGCA AAAATGACATCAACCTCTTGAAATGTGGCAGCATTCGCCTTGGGGAAAAGGATGCACATTCACAAGGTGA GGTGGTATCATGTTTGGAAAAAGGCCTGGTGAAAGAGGCAGAAGAGAAAGAGCCCAAGATTCAAGTCTCA GAACTCTGCAAGAAAGCCATTCTTAGGGTGGCTGAGCTGTCCTCGGATGACTTTCATTTAGACCGGCATT TGTATTTTGCTTGCAGAGATGATCGGGAGCGCTTTTGTGAGAATACACAAGCTGGTGAAGGAAGAGTGTA TAAATGCCTTTTTAACCATAAATTTGAAGAATCCATGAGTGAAAAGTGTAGAGAAGCACTAACTACACGG CAGAAGCTCATTGCCCAGGATTATAAAGTCAGCTATTCATTGGCCAAGTCCTGTAAGAGTGACTTGAAAA AATACCGATGTAATGTGGAAAACCTTCCTCGCTCCCGAGAGGCCAGGCTCTCCTACCTTCTGATGTGCCT GGAGTCAGCCGTGCACAGAGGGCGACAAGTGAGCAGTGAGTGCCAAGGTGAGATGCTGGATTACCGACGC ATGCTGATGGAAGACTTCTCTCTGAGCCCCGAGATCATCCTGAGCTGTCGAGGGGAGATCGAACACCACT GTTCTGGATTACATCGGAAAGGACGCACCCTCCACTGTCTGATGAAAGTAGTCCGGGGTGAAAAGGGGAG CCTTGGAATGAACTGCCAGCAGGCGCTTCAGACACTGATTCAGGAGACTGACCCTGGCGCAGACTACCGC ATTGATCGAGCTTTGAATGAAGCTTGTGAATCTGTAATACAGACAGCCTGCAAACATATACGATCCGGAG ACCCAATGATTCTTTCATGTCTGATGGAACATTTATACACAGAGAAAATGGTGGAAGACTGTGAACACCG TCTCTTAGAGCTACAGTATTTTATCTCTCGGGATTGGAAGTTGGACCCTGTTTTATACCGGAAATGCCAG GGAGATGCTTCCCGTCTTTGCCACACCCATGGTTGGAATGAGACCAGTGAACTTATGCCTCCCGGAGCTG TGTTTTCTTGCCTGTATAGACATGCCTACCGCACAGAAGAGCAAGGACGGAGGCTCTCACGAGAATGCCG AGCTGAAGTCCAGAGGATCCTACACCAGCGTGCCATGGATGTTAAGCTGGATCCTGCCCTCCAGGACAAG TGCCTGATAGATCTAGGAAAGTGGTGCAGTGAGAAGACAGAGACTGGTCAGGAGCTTGAGTGCCTTCAGG ACCATCTGGATGACTTAGCTGTGGAATGCAGAGACATAGTGGGCAACCTCACGGAGTTAGAGTCTGAGGA CATACAAATAGAAGCTTTGCTGATGAGAGCCTGTGAGCCTATAATTCACAACTTTTGCCACGATGTGGCA GACAACCAGATTGACTCTGGGGACCTAATGGAGTGTCTGATCCAGAACAAGCACCAGAAGGACATGAATG AGAAGTGCGCCATTGGCGTCACTCACTTCCAGCTGGTCCAAATGAAGGATTTTCGATTCTCTTACAAGTT CAAAATGGCCTGCAAGGAGGATGTGTTAAAACTTTGCCCCAACATAAAAAAGAAGGTGGATGTGGTGATC TGCCTGAGCACCACCGTGCGCAACGACACTCTGCAGGAGGCCAAGGAGCACCGGGTGTCTCTCAAGTGCC GCAAGCAGCTCCGTGTGGAGGAGCTGGAGATGACAGAGGACATCCGTTTGGAACCAGATCTGTATGAAGC CTGCAAGAGTGACATCAAGAACTACTGTTCTACAGTGCAATATGGAAATGCTCAGATTATTGAATGTCTA AAAGAAAACAAGAAGCAACTGAGTACCCGTTGTCACCAGAGAGTATTTAAGCTGCAGGAGACAGAGATGA TGGACCCAGAACTAGACTATACACTCATGAGAGTCTGCAAGCAGATGATTAAGCGGTTCTGTCCAGAAGC AGATTCTAAAACTATGTTACAGTGTTTGAAACAAAACAAGAACAGTGAATTGATGGACCCCAAGTGCAAA CAGATGATCACCAAGCGTCAGATCACCCAGAACACAGATTACCGTTTAAACCCCGTGCTAAGGAAGGCCT GTAAAGCTGACATTCCTAAATTCTGTCATGGCATCCTGACCAAGGCCAAGGATGATTCAGAGCTAGAAGG CCAAGTTATCTCATGCCTCAAGCTGAGATACGCTGACCAGCGCCTGTCTTCAGACTGTGAGGATCAGATC CGTATCATCACCCAAGAGTCTGCGCTAGATTACCGCCTAGACCCTCAGCTCCAGCTGCACTGCTCAGACG AGATTGCTAATTTATGTGCTGAAGAAGCAGCAGCCCAGGAACAGACAGGCCAAGTGGAAGAGTGCCTAAA GGTCAACCTGCTCAAGATCAGGACAGAGCTGTGTAAAAAGGAAGTGTTGAACATGTTGAAGGAAAGCAAA GCAGACATCTTTGTGGATCCAGTTCTTCACACAGCGTGTGCCTTGGACATTAAACACCACTGTGCAGCCA TCACCCCCGGCCGTGGGCGTCAGATGTCCTGCCTGATGGAGGCCTTGGAAGACAAGCGCGTGAGGTTGCA GCCCGAGTGCAAAAAGCGCCTGAATGACCGAATCGAGATGTGGAGTTATGCAGCCAAGGTGGCCCCAGCG GACGGCTTCTCTGATCTTGCCATGCAAGTGATGACATCCCCATCAAAGAATTACATCCTGTCTGTGATCA GCGGGAGCATCTGCATATTGTTCCTGATTGGCCTGATGTGTGGACGGATCACTAAGAGGGTGACACGAGA GCTGAAGGACAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017211
Insert Size	3516 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017211.2, NP_058907.1
RefSeq Size	5292 bp
RefSeq ORF	3516 bp
Locus ID	29476
UniProt ID	Q62638
Gene Summary	a Golgi resident sialoglycoprotein that binds basic fibroblast growth factor [RGD, Feb 2006]

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