Bcdin3d (NM_001108751) Rat Tagged ORF Clone
CAT#: RR206088
- TrueORF®
Bcdin3d (Myc-DDK-tagged ORF) - Rat BCDIN3 domain containing (Bcdin3d), (10 ug)
"NM_001108751" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | RGD1306433 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR206088 representing NM_001108751
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAACCCAGGAATTGTCCAAAGGGGGCGTTGAAGAGGCTGTGGAGGAAGACGACCCGGCAGCTT TGAAGCCTGGGGCCGCGCCGTTCGGAAATTTCCCTCATTATTCCCGCTTCCACCCTCCCGAGCAAAGGCT CCGCCTTCTGCCCCCGGAGCTTCTTCGACAGCTCTTCCCTCCCGAGGGTCCCGAGAGGAGGCCGATCCTA GGGCTCGACGTGGGGTGTAACTCCGGGGATCTGAGCATGGCTCTGTATAAACATTTCCTTTCCCCGCATG ATGGGGAGACCAGCTCCGGCACTTCCAGAGAACTCCGCCTCCTCTGCTGTGATATAGATCCAGTCCTTGT GGAGCGGGCTGAAAATGGCTGTCGCTTTCCTGATGCCTTGACCTTTATCACCCTGGACATCATGGATCAA GAGAGCAGGAAGGTTCCCTTGAGTTCTTTCTTAAGCCAGTTTGGGCGTTCCGTTTTTGACATTGTCTTCT GCATGTCAGTAACCATGTGGATTCACCTGAACCATGGAGACCGTGGTCTGTGCGAGTTCCTGGCCCACGT CTCCTCTCTCTGCAGCTACCTCCTCGTGGAGCCACAGCCCTGGAAGTGTTACCGGGCAGCCGCAAGACGC CTACGCAAGCTGGGACTCCACAATTTTGATCACTTCCGCTCCCTGGCCATCCGAGGTGACATGGCCAGCC AGATCGTGCGAATCTTGACGCAGGACCACGGGATGGAGTTAGCATGCTGTTTTGGCAACACCAGTTGGGA CCGAAGCCTTCTTCTCTTCAGAGCAAAGCACACCGAGGAGACTCAGGCAATCCCTGAATCGTCAACCAAA GAGACAGGGACGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR206088 representing NM_001108751
Red=Cloning site Green=Tags(s) MAATQELSKGGVEEAVEEDDPAALKPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPPEGPERRPIL GLDVGCNSGDLSMALYKHFLSPHDGETSSGTSRELRLLCCDIDPVLVERAENGCRFPDALTFITLDIMDQ ESRKVPLSSFLSQFGRSVFDIVFCMSVTMWIHLNHGDRGLCEFLAHVSSLCSYLLVEPQPWKCYRAAARR LRKLGLHNFDHFRSLAIRGDMASQIVRILTQDHGMELACCFGNTSWDRSLLLFRAKHTEETQAIPESSTK ETGTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001108751 |
ORF Size | 855 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001108751.1, NP_001102221.1 |
RefSeq Size | 1254 bp |
RefSeq ORF | 858 bp |
Locus ID | 363001 |
UniProt ID | D4ABH7 |
MW | 32 kDa |
Gene Summary | O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA, acting as a capping enzyme. Less efficiently, also methylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs. There is some controversy about the methylation of pre-miR-145, since the dimethylation first described as the specific enzymatic activity cannot be reproduced by a more recent work which observes a monomehtylation of pre-miR-145 but two orders weaker than the methylation of cytosolic histidyl tRNA.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN206088 | Bcdin3d (untagged ORF) - Rat BCDIN3 domain containing (Bcdin3d), (10 ug) |
CNY 3,990.00 |
|
RR206088L3 | Lenti ORF clone of Bcdin3d (Myc-DDK-tagged ORF) - Rat BCDIN3 domain containing (Bcdin3d), (10 ug) |
CNY 6,080.00 |
|
RR206088L4 | Lenti ORF clone of Bcdin3d (mGFP-tagged ORF) - Rat BCDIN3 domain containing (Bcdin3d), (10 ug) |
CNY 6,650.00 |
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