Kcnma1 (NM_031828) Rat Tagged ORF Clone
CAT#: RR202293
- TrueORF®
Kcnma1 (Myc-DDK-tagged ORF) - Rat potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), (10 ug)
"NM_031828" in other vectors (1)
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CNY 8,656.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BKCa; cbv1; Kcnma; KCNMA1b; KCNMA1c; Slo |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202293 representing NM_031828
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACGGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCG GAGGCGAGACGGCTCTTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCTTC TTCCTCCTCCTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTC ATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCT CCTCCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACTCTCAAGTACCTGTGGAC CGTTTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGAT GGTACTCTCAAGCCAGTGGATGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGA AGGACTGGGCAGGGGTGATGATATCTGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGC TCTCAGCATTGGTGCCCTTGTAATATACTTCATAGACTCATCAAACCCAATAGAATCCTGCCAGAATTTC TACAAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTTGGCTTGCGGT TTATTGCAGCCAATGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCTGTAGTAGATTTCTTCACAGTCCC TCCCGTGTTTGTGTCTGTATACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTG ATTCAGTTTTCAGAAATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAATTCCATCAAGCTGGTGAACC TGCTCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGA CCCATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACA ATGTCTACAGTGGGTTACGGGGATGTTTATGCAAAAACCACGCTTGGACGCCTCTTCATGGTCTTCTTCA TCCTCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAA ATACGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGGCACATTACTCTGGAG AGTGTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTTTTTC TTCACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTA TCAGGGCTCTGTCCTCAATCCACACGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGTTTGATC CTTGCCAATAAGTACTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATAA AGAACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAA CATCCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATA GCCCAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAA AGATTGAGGAAGATACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCT TTCCAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTG ATGATAGCCATTGAGTACAAGTCTGCCAACAGGGAGAGCCGAAGCCGAAAGCGAATATTAATTAACCCTG GGAACCACCTTAAGACCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAG GGCATTTTTTTACTGCAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGCGGCTGC AGGCGGCTGATCTATTCCAAGATGTCCATCTACAAGAGAATGAGCCGAGCATGTTGTTTTGATTGCGGAC GTTCTGAGCGTGACTGCTCGTGCATGTCAGGCCGTGTGCGTGGTAACGTGGACACCCTTGAGAGAAACTT CCCGCTTTCTTCTGTCTCTGTTAATGATTGCTCCACCAGTTTCCGTGCCTTTGAAGATGAGCAACCGCCA ACACTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCACCCAACACATCCCCGAAGCTGA TGAGGCATGACCCCTTGTTAATTCCTGGCAACGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTA CGACTCCACTGGAATGTTTCACTGGTGCGCACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAA GCTGCCATGACTGTCCTGAGTGGTCATGTTGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTG GCCTCCGGAACCTGGTGATGCCGCTTCGTGCTAGCAATTTTCACTATCATGAGCTCAAACACATTGTGTT TGTGGGCTCCATCGAGTACCTCAAGCGGGAGTGGGAAACACTGCACAACTTCCCCAAAGTATCCATATTG CCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCC TGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAA CATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCT GGAATGGACAGATCATCACCTGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACCG GAGTCAACATTCCCATCATCACGGAACTCGTGAACGATACTAATGTTCAGTTTTTGGACCAAGATGATGA TGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTTAGCGTC CTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAG GAGGAGCCACGCCAGAGCTCGAAGCTTTAATAGCTGAGGAGAATGCACTTCGAGGGGGTTACAGCACTCC GCAGACACTAGCCAACAGGGACCGCTGCCGAGTGGCCCAGTTAGCCCTGTTGGATGGGCCCTTTGCGGAC TTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAACATGCTTTGTTTTG GAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAA TCCCCCCTACGAGTTTGAACTTGTACCGACAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCT GGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCTG TCCACTCCATCCCGTCCACAGCAAATCGGCCAAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAA AAAAGAAATGGTTTACAGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RR202293 representing NM_031828
Red=Cloning site Green=Tags(s) MANGGGGGGGGSSGSSGGGGGGGGGETALRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSVHEPKMDAL IIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQAD GTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNF YKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRL IQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVT MSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLE SVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLI LANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFI AQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLL MIAIEYKSANRESRSRKRILINPGNHLKTQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGC RRLIYSKMSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTLERNFPLSSVSVNDCSTSFRAFEDEQPP TLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSE AAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSIL PGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPP GMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSV LDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFAD LGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNA GQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031828 |
ORF Size | 3729 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031828.1, NP_114016.1 |
RefSeq Size | 5453 bp |
RefSeq ORF | 3732 bp |
Locus ID | 83731 |
UniProt ID | Q62976 |
MW | 138.4 kDa |
Gene Summary | large-conductance Ca(2+)-activated K(+) channel; may be involved in the shortened duration of action potential in hippocampal CA1 pyramidal neurons and in channel activity in carotid body chemoreceptor cells [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202293 | Kcnma1 (untagged ORF) - Rat potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), (10 ug) |
CNY 19,950.00 |