Kcnma1 (NM_031828) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BKCa; cbv1; Kcnma; KCNMA1b; KCNMA1c; Slo
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202293 representing NM_031828 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACGGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCG GAGGCGAGACGGCTCTTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCTTC TTCCTCCTCCTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTC ATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCT CCTCCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACTCTCAAGTACCTGTGGAC CGTTTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGAT GGTACTCTCAAGCCAGTGGATGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGA AGGACTGGGCAGGGGTGATGATATCTGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGC TCTCAGCATTGGTGCCCTTGTAATATACTTCATAGACTCATCAAACCCAATAGAATCCTGCCAGAATTTC TACAAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTTGGCTTGCGGT TTATTGCAGCCAATGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCTGTAGTAGATTTCTTCACAGTCCC TCCCGTGTTTGTGTCTGTATACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTG ATTCAGTTTTCAGAAATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAATTCCATCAAGCTGGTGAACC TGCTCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGA CCCATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACA ATGTCTACAGTGGGTTACGGGGATGTTTATGCAAAAACCACGCTTGGACGCCTCTTCATGGTCTTCTTCA TCCTCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAA ATACGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGGCACATTACTCTGGAG AGTGTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTTTTTC TTCACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTA TCAGGGCTCTGTCCTCAATCCACACGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGTTTGATC CTTGCCAATAAGTACTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATAA AGAACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAA CATCCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATA GCCCAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAA AGATTGAGGAAGATACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCT TTCCAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTG ATGATAGCCATTGAGTACAAGTCTGCCAACAGGGAGAGCCGAAGCCGAAAGCGAATATTAATTAACCCTG GGAACCACCTTAAGACCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAG GGCATTTTTTTACTGCAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGCGGCTGC AGGCGGCTGATCTATTCCAAGATGTCCATCTACAAGAGAATGAGCCGAGCATGTTGTTTTGATTGCGGAC GTTCTGAGCGTGACTGCTCGTGCATGTCAGGCCGTGTGCGTGGTAACGTGGACACCCTTGAGAGAAACTT CCCGCTTTCTTCTGTCTCTGTTAATGATTGCTCCACCAGTTTCCGTGCCTTTGAAGATGAGCAACCGCCA ACACTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCACCCAACACATCCCCGAAGCTGA TGAGGCATGACCCCTTGTTAATTCCTGGCAACGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTA CGACTCCACTGGAATGTTTCACTGGTGCGCACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAA GCTGCCATGACTGTCCTGAGTGGTCATGTTGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTG GCCTCCGGAACCTGGTGATGCCGCTTCGTGCTAGCAATTTTCACTATCATGAGCTCAAACACATTGTGTT TGTGGGCTCCATCGAGTACCTCAAGCGGGAGTGGGAAACACTGCACAACTTCCCCAAAGTATCCATATTG CCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCC TGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAA CATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCT GGAATGGACAGATCATCACCTGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACCG GAGTCAACATTCCCATCATCACGGAACTCGTGAACGATACTAATGTTCAGTTTTTGGACCAAGATGATGA TGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTTAGCGTC CTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAG GAGGAGCCACGCCAGAGCTCGAAGCTTTAATAGCTGAGGAGAATGCACTTCGAGGGGGTTACAGCACTCC GCAGACACTAGCCAACAGGGACCGCTGCCGAGTGGCCCAGTTAGCCCTGTTGGATGGGCCCTTTGCGGAC TTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAACATGCTTTGTTTTG GAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAA TCCCCCCTACGAGTTTGAACTTGTACCGACAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCT GGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCTG TCCACTCCATCCCGTCCACAGCAAATCGGCCAAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAA AAAAGAAATGGTTTACAGATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_031828
Insert Size	3732 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031828.1, NP_114016.1
RefSeq Size	5453 bp
RefSeq ORF	3732 bp
Locus ID	83731
UniProt ID	Q62976
Gene Summary	large-conductance Ca(2+)-activated K(+) channel; may be involved in the shortened duration of action potential in hippocampal CA1 pyramidal neurons and in channel activity in carotid body chemoreceptor cells [RGD, Feb 2006]

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