Wnk3 (NM_001163607) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1563131
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217683 representing NM_001163607 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTCAAA GCAGGGTTGCCCGGGCTGTTGCACCTCTGACTGTAGAAGCTAGAATAAAGGAAAAATACAGTACCTTTTC TGCTTCTGGGGAAACCATTGAAAGAAAGAGATTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA GTTGCCCAATCTTCCCGCAGAGATGAAAGAAAAGTTGCAACAAATATTTCAAGAGTAGAAAAAGTTCCTA CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAGTGTTCAAAGGCAACCTCAGAATCCTCAAA AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTCGCAACGTCC CCTAGTGGCAGGTTTCTAAAATTTGATATAGAGCTGGGAAGAGGCGCTTTTAAAACAGTATATAAAGGAT TGGACACTGAAACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA GCAACGGTTCAAAGAAGAAGCTGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTTTACGAT TCATGGGAGTCCATATTGAAAGGAAAGAAATGTATTGTTTTAGTGACTGAACTAATGACATCAGGAACCT TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATTTT AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCCT TTGCTAAGAGTGTCATTGGCACTCCTGAGTTTATGGCGCCAGAGATGTATGAAGAACACTATGATGAATC TGTAGATGTTTATGCTTTTGGGATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC CAGAATGCAGCACAAATATACCGTAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCGATCAAGGA TCTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGTGTGACACTGATT AAAAAGATAAGGGAAAAGAAGCCTGCTGGATGCTTAGAGGAACGCAGGGATTCCCAGTGCAAGCATGCGA GGAATGTAAATCCTCAGCAGCAGACTGCAACTTTACGACCTGCTCCTGGTCCACACAATGCGGCTGAGTG TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGCTTGTACAAGGCAAAGCACAGCAGCAGCCCTCC TCTGTTAGGGGTGACACTTCATCTGAACCAGCAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCACC CCACTGTAGCCTATTCTTCAAACCAGGCAACTAGTTCTCAGGAGCAGCCAAAGCTGACTCGGTCTCCAGT TTTGCCTGTGGTTCAAGGTCAGTCTTCTGTTATGCCCATATATGCTGCTGGACCTGCAGTTGCTTCACAG TCCCAGATTTCTCCATTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG AACAACAAGCAACTCCTCCAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA AAACACCAATAGCCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGATCAAACAAAGAAGAGCCTCC TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGGGACAACA TGGTGGAATGTCAGCTGGAAACACATAACAACAAGATGGTCACATTCAAGTTTGATGTTGATGGTGATGC ACCTGAGGATATCGCTGACTATATGACAGGATCATCTGAACAAGTATTGATAAATTCTGCATCCACTCAA ACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGTATCAACCAAACAATAA AAAATCGTGAGGCGCAGTCTCCTCCTTCCCTTCAGCCTTCCGTGGCTATGGTGCCTGGGTTACATCCATT TCCTAATAACACAAGTAATCAAGAAATATCACAGGACACACTGTTCACTGTTCCAGGCCATCATGTAGTT TTCACCTCCAAGTTAGATGGTAAGATTGGTGAACGTGCTAGTGTAGAAACGGAGCAGACGAGTGTTCCCT ATCAAGCAGAAGATGACAAACTAAAGGCACCAGCTACTGATACTTCCAATTATTCTGCTACTTTGGTGTG TCCAGATCCAGCTGGATGTGAGGCACTCACCAGCCAAGCAGGCATGTTCATACCTACATATCCATGTCAG CAAGCTGCTGTTCCGGCTGATGTGCTTATGTCCCATCCTGGTGAATCAGTTCAGATTGGCAGTAATGCTG TTGTAACGTCAGTACTTGTATCTTCTGATCAAAAGCCTCAATCCTTATCAGTACAGCAGCCAACTATAGA TGCAGAGTTTATTTCACAAGAAGGAGAAACCACAGTAAACTCTGAAACGAGTTCTCCTAAGGCTGTCGGT GCCACTCAGACTCCTGGCTTTGAACCAACTGTGCTTCTACCTGCGACTATCCTGGAATCAGATGGGGAAC GACCTCCAAAAATGGAGTTTACAGACAATCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTA TCAAGAGCACAGCATTTCTACCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTT TCTTCCTCTGCTGAAGATATACTATCCTCCCCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTC AAGATAGCCAAGCACAATCCCCGAATTTCCAACCAACAGGCTCTAAGATTCTATCGAATGTGGCTGCAAG TCAGCCTGCTCATATACCAGTGCTCAAAAGAGACCTGAATGTGATAACTTCTGTACCCAGCGAGCTATGT TTACACGAGATGTCCCCAGATGCTTCACTTCCAGGGGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCG ATGGAACCATTCATCTGCAGACAGGAGGTGGATATTTTGGCCTAAGCTTTACTTGTCCTAGTCTCAAAAA TCCTATTAGCAGGAAATCCTGGACTCGAAAATTAAAAAGCTGGGCATACAGGCTACGGCAGTCAACCAGC TTTTTCAAGAGATCAAAAGTTCGTCAAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCA TTCCGCTGACACGGGAGTTCTCAGCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATT TCAGCGGGGTCGGTTCCAGGTGATTACAGTTCCTCAGCAGCAGCCAGTGAAAATGATGTCTTTTGGAAAA GAACATAGACCGCCATCGAAGAAAACGACAGCCCAGTCTAACGAGCAAGCACTAACCTTTACTGAAGCTG CAGTGACACAGTTGATCGAAGTGGAACCTGCCATACCCACTCATAAAGCTTCAGTTTCTTCACAAAAGTT ACGAACTCTTTATGAAACCTTTAAAGAAGATAAAGGAGACCCTGAACAAGGTGACATCTTATCTTTGAGC ACAGCTCGTGAGACCAGTGTGTCTTCAGTGACAACAGAAAAGAATGTGGAAGAAACTTCTACTACAGGAA TTAGTGTGCAGTCTGGGTCTGAAATGTTAGATAAAGAAAAAGATGAATCAACTCCTGGGAAACAGACATG TACTAATGAATTTTCAGCCACTCCTGCTGGCAGTGGAAAGTCAGTGGCAAAAGCTGCTCCAAAGAGTGGT CAGCATTTACCAGCCCGTGCTCAAACACAGAGTTCACTCTTCTACTCTCCATCTTCTCCAATGAGCAGTG ACAATGAATCAGAAATCGAGGATGAGGACTTGAAAGCGGAGCTTCAAAGATTACGGGAAAAACACATTCA AGAGGTGGTCAGTCTTCAAACACAGCAGAATAAAGAGCTACAGGAGCTCTATGAACGCCTTCGAGCAACT AAAGACAGCAAAGCGCAATCTTCAGAAGTTCCCATGCCACCTGCATCACCACGCAGGCCAAGATCCTTCA AAAGCAAACTTCGAAGCCGCCCCCAGTCCTTGACACATTCGGACAATCTCGTTGTTGTAAAAGATGTGCA GCGTGCGGAGAGTAATACTGCGCCACGCCAGCAATCGCCAGCCAGTAAGAAGGGGATGTTCACAGATGAT TTACACAAGCTGGTGGATGACTGGACAAGAGAAACAGTAGGAAATTTTCCTAGCAAGCCAAGTTTAAACC AACTCAAACAGAGCCAACAAAAATCAGAGGCAGAAAACTGGAACAAGTTGCATGAAAGCACTCCATCTAC TGTGGGATACACATCAACCTGGATTTCTTCTCTCTCCCAAATTCGCGGAGCTGCCCCGTCTTCCTTGCCA CATGGACTCCCACTCCCTTCATTTCCTGGGCCATTAGCGTCCTATGGAATGCCTCATGCTTGTCAGTACA ATCCCGTAGGCGCCGCAAGCTATCCAGTACAGTGGGTTGGGATTTCGGGAGCAGCACAGCAATCCGTAGT GCTTCCTACCCAATCTGGGGGACTCTTCCAGCCTGGGATGAATCTGCAGTCATTTCCAGCTCCACCAGTG CAGAACCCACCTTCAATCCCTCCTGGTCCCAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001163607
Insert Size	5076 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001163607.1, NP_001157079.1
RefSeq Size	5076 bp
RefSeq ORF	5076 bp
Locus ID	317420

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