Wnk3 (NM_001163607) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1563131
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217683 representing NM_001163607 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGATTCTGGGGAGCCAGCTAGCACAGAAGATTCTGAGAAGCCTGATGGAGTTTCCTTTCAAA GCAGGGTTGCCCGGGCTGTTGCACCTCTGACTGTAGAAGCTAGAATAAAGGAAAAATACAGTACCTTTTC TGCTTCTGGGGAAACCATTGAAAGAAAGAGATTTTTCCGAAAGAGTGTTGAGATGACAGAAGATGACAAA GTTGCCCAATCTTCCCGCAGAGATGAAAGAAAAGTTGCAACAAATATTTCAAGAGTAGAAAAAGTTCCTA CAAATGTGCTAAGAGGTGGGCAAGAAGTTAAATATGAGCAGTGTTCAAAGGCAACCTCAGAATCCTCAAA AGATTGCTTCAAGGAGAAAACTGAAAAGGAAATGGAAGAAGAAGCAGAAATGAAGGCTGTCGCAACGTCC CCTAGTGGCAGGTTTCTAAAATTTGATATAGAGCTGGGAAGAGGCGCTTTTAAAACAGTATATAAAGGAT TGGACACTGAAACATGGGTTGAAGTTGCTTGGTGTGAGCTACAGGACCGCAAATTAACTAAAGCTGAGCA GCAACGGTTCAAAGAAGAAGCTGAGATGTTGAAAGGCCTCCAGCATCCCAATATAGTTCGATTTTACGAT TCATGGGAGTCCATATTGAAAGGAAAGAAATGTATTGTTTTAGTGACTGAACTAATGACATCAGGAACCT TAAAGACGTACTTAAAACGATTTAAAGTCATGAAACCAAAGGTCTTAAGAAGCTGGTGCAGGCAAATTTT AAAAGGATTGCAGTTCTTACACACAAGGACGCCTCCTATTATTCACCGGGATCTGAAATGTGACAACATT TTCATCACAGGACCCACTGGATCTGTGAAGATTGGTGATCTGGGACTTGCAACCTTAATGCGCACATCCT TTGCTAAGAGTGTCATTGGCACTCCTGAGTTTATGGCGCCAGAGATGTATGAAGAACACTATGATGAATC TGTAGATGTTTATGCTTTTGGGATGTGTATGTTGGAAATGGCTACTTCGGAGTATCCATATTCTGAGTGC CAGAATGCAGCACAAATATACCGTAAAGTAACCAGTGGCATAAAACCAGCCAGCTTCAATAAAGTCACAG ACCCTGAAGTGAAAGAAATAATTGAAGGATGTATTAGACAAAACAAATCTGAAAGGTTATCGATCAAGGA TCTACTAAACCATGCATTCTTTGCTGAGGATACTGGACTGAGGGTGGAGTTAGCAGAGGAAGATGATTGC TCAAATTCATCCTTGGCTCTAAGACTCTGGGTTGAAGATCCTAAGAAATTAAAAGGCAAACATAAAGACA ATGAAGCTATTGAATTCAGTTTCAACTTAGAAGCAGATACACCTGAGGAAGTAGCGTATGAAATGGTCAA GTCTGGATTCTTCCATGAAAGTGATTCCAAAGCTGTTGCTAAGTCCATTAGGGACCGTGTGACACTGATT AAAAAGATAAGGGAAAAGAAGCCTGCTGGATGCTTAGAGGAACGCAGGGATTCCCAGTGCAAGCATGCGA GGAATGTAAATCCTCAGCAGCAGACTGCAACTTTACGACCTGCTCCTGGTCCACACAATGCGGCTGAGTG TGAAGAAACAGAAGTTGATCAACATGTTCGACAGCAGCTTGTACAAGGCAAAGCACAGCAGCAGCCCTCC TCTGTTAGGGGTGACACTTCATCTGAACCAGCAGCTGGACCAGTTCTACATTCAGATACTTCAAGTCACC CCACTGTAGCCTATTCTTCAAACCAGGCAACTAGTTCTCAGGAGCAGCCAAAGCTGACTCGGTCTCCAGT TTTGCCTGTGGTTCAAGGTCAGTCTTCTGTTATGCCCATATATGCTGCTGGACCTGCAGTTGCTTCACAG TCCCAGATTTCTCCATTAACTATCCAGAAGGTCTCACAGATAAAGCCTGTATCCCAACCAATTGGAGCTG AACAACAAGCAACTCCTCCAAATCCAGATTTTGTTCGAAGCTTGAATCAAGATGTGACATCTGTGAAAGA AAACACCAATAGCCCTGATACCCCAAGTGGAAATGGCAAACAGGATCGGATCAAACAAAGAAGAGCCTCC TGTCCCCGACCAGAGAAGGGGACTAAATTCCAGCTTACTGTCCTTCAGGTCTCAGTCTCTGGGGACAACA TGGTGGAATGTCAGCTGGAAACACATAACAACAAGATGGTCACATTCAAGTTTGATGTTGATGGTGATGC ACCTGAGGATATCGCTGACTATATGACAGGATCATCTGAACAAGTATTGATAAATTCTGCATCCACTCAA ACTAGCAATGAATCTGCTCCTCAGTCATCCCCAGTTGGTCGCTGGCGGTTTTGTATCAACCAAACAATAA AAAATCGTGAGGCGCAGTCTCCTCCTTCCCTTCAGCCTTCCGTGGCTATGGTGCCTGGGTTACATCCATT TCCTAATAACACAAGTAATCAAGAAATATCACAGGACACACTGTTCACTGTTCCAGGCCATCATGTAGTT TTCACCTCCAAGTTAGATGGTAAGATTGGTGAACGTGCTAGTGTAGAAACGGAGCAGACGAGTGTTCCCT ATCAAGCAGAAGATGACAAACTAAAGGCACCAGCTACTGATACTTCCAATTATTCTGCTACTTTGGTGTG TCCAGATCCAGCTGGATGTGAGGCACTCACCAGCCAAGCAGGCATGTTCATACCTACATATCCATGTCAG CAAGCTGCTGTTCCGGCTGATGTGCTTATGTCCCATCCTGGTGAATCAGTTCAGATTGGCAGTAATGCTG TTGTAACGTCAGTACTTGTATCTTCTGATCAAAAGCCTCAATCCTTATCAGTACAGCAGCCAACTATAGA TGCAGAGTTTATTTCACAAGAAGGAGAAACCACAGTAAACTCTGAAACGAGTTCTCCTAAGGCTGTCGGT GCCACTCAGACTCCTGGCTTTGAACCAACTGTGCTTCTACCTGCGACTATCCTGGAATCAGATGGGGAAC GACCTCCAAAAATGGAGTTTACAGACAATCGAATTAAAACACTGGATGAAAAATTAAGAAACCTGCTCTA TCAAGAGCACAGCATTTCTACCATCTGTCCTGAGAGTCAGAAGGATACTCAAAGCATAGACTCTCCATTT TCTTCCTCTGCTGAAGATATACTATCCTCCCCAATGCCAGAAGTCATAGCCATCAGTCACTGTGGGATTC AAGATAGCCAAGCACAATCCCCGAATTTCCAACCAACAGGCTCTAAGATTCTATCGAATGTGGCTGCAAG TCAGCCTGCTCATATACCAGTGCTCAAAAGAGACCTGAATGTGATAACTTCTGTACCCAGCGAGCTATGT TTACACGAGATGTCCCCAGATGCTTCACTTCCAGGGGATCCAGAGGCCTATCCTGCTGCTGTGTCAAGCG ATGGAACCATTCATCTGCAGACAGGAGGTGGATATTTTGGCCTAAGCTTTACTTGTCCTAGTCTCAAAAA TCCTATTAGCAGGAAATCCTGGACTCGAAAATTAAAAAGCTGGGCATACAGGCTACGGCAGTCAACCAGC TTTTTCAAGAGATCAAAAGTTCGTCAAGTGGAAACAGAAGATAAAAGATCAGCAATTGCTTCTGATCCCA TTCCGCTGACACGGGAGTTCTCAGCTGATACTAGGGCTTTGAGTAGATGTAAAGCGATGAGTGGATCATT TCAGCGGGGTCGGTTCCAGGTGATTACAGTTCCTCAGCAGCAGCCAGTGAAAATGATGTCTTTTGGAAAA GAACATAGACCGCCATCGAAGAAAACGACAGCCCAGTCTAACGAGCAAGCACTAACCTTTACTGAAGCTG CAGTGACACAGTTGATCGAAGTGGAACCTGCCATACCCACTCATAAAGCTTCAGTTTCTTCACAAAAGTT ACGAACTCTTTATGAAACCTTTAAAGAAGATAAAGGAGACCCTGAACAAGGTGACATCTTATCTTTGAGC ACAGCTCGTGAGACCAGTGTGTCTTCAGTGACAACAGAAAAGAATGTGGAAGAAACTTCTACTACAGGAA TTAGTGTGCAGTCTGGGTCTGAAATGTTAGATAAAGAAAAAGATGAATCAACTCCTGGGAAACAGACATG TACTAATGAATTTTCAGCCACTCCTGCTGGCAGTGGAAAGTCAGTGGCAAAAGCTGCTCCAAAGAGTGGT CAGCATTTACCAGCCCGTGCTCAAACACAGAGTTCACTCTTCTACTCTCCATCTTCTCCAATGAGCAGTG ACAATGAATCAGAAATCGAGGATGAGGACTTGAAAGCGGAGCTTCAAAGATTACGGGAAAAACACATTCA AGAGGTGGTCAGTCTTCAAACACAGCAGAATAAAGAGCTACAGGAGCTCTATGAACGCCTTCGAGCAACT AAAGACAGCAAAGCGCAATCTTCAGAAGTTCCCATGCCACCTGCATCACCACGCAGGCCAAGATCCTTCA AAAGCAAACTTCGAAGCCGCCCCCAGTCCTTGACACATTCGGACAATCTCGTTGTTGTAAAAGATGTGCA GCGTGCGGAGAGTAATACTGCGCCACGCCAGCAATCGCCAGCCAGTAAGAAGGGGATGTTCACAGATGAT TTACACAAGCTGGTGGATGACTGGACAAGAGAAACAGTAGGAAATTTTCCTAGCAAGCCAAGTTTAAACC AACTCAAACAGAGCCAACAAAAATCAGAGGCAGAAAACTGGAACAAGTTGCATGAAAGCACTCCATCTAC TGTGGGATACACATCAACCTGGATTTCTTCTCTCTCCCAAATTCGCGGAGCTGCCCCGTCTTCCTTGCCA CATGGACTCCCACTCCCTTCATTTCCTGGGCCATTAGCGTCCTATGGAATGCCTCATGCTTGTCAGTACA ATCCCGTAGGCGCCGCAAGCTATCCAGTACAGTGGGTTGGGATTTCGGGAGCAGCACAGCAATCCGTAGT GCTTCCTACCCAATCTGGGGGACTCTTCCAGCCTGGGATGAATCTGCAGTCATTTCCAGCTCCACCAGTG CAGAACCCACCTTCAATCCCTCCTGGTCCCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217683 representing NM_001163607 Red=Cloning site Green=Tags(s) MATDSGEPASTEDSEKPDGVSFQSRVARAVAPLTVEARIKEKYSTFSASGETIERKRFFRKSVEMTEDDK VAQSSRRDERKVATNISRVEKVPTNVLRGGQEVKYEQCSKATSESSKDCFKEKTEKEMEEEAEMKAVATS PSGRFLKFDIELGRGAFKTVYKGLDTETWVEVAWCELQDRKLTKAEQQRFKEEAEMLKGLQHPNIVRFYD SWESILKGKKCIVLVTELMTSGTLKTYLKRFKVMKPKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNI FITGPTGSVKIGDLGLATLMRTSFAKSVIGTPEFMAPEMYEEHYDESVDVYAFGMCMLEMATSEYPYSEC QNAAQIYRKVTSGIKPASFNKVTDPEVKEIIEGCIRQNKSERLSIKDLLNHAFFAEDTGLRVELAEEDDC SNSSLALRLWVEDPKKLKGKHKDNEAIEFSFNLEADTPEEVAYEMVKSGFFHESDSKAVAKSIRDRVTLI KKIREKKPAGCLEERRDSQCKHARNVNPQQQTATLRPAPGPHNAAECEETEVDQHVRQQLVQGKAQQQPS SVRGDTSSEPAAGPVLHSDTSSHPTVAYSSNQATSSQEQPKLTRSPVLPVVQGQSSVMPIYAAGPAVASQ SQISPLTIQKVSQIKPVSQPIGAEQQATPPNPDFVRSLNQDVTSVKENTNSPDTPSGNGKQDRIKQRRAS CPRPEKGTKFQLTVLQVSVSGDNMVECQLETHNNKMVTFKFDVDGDAPEDIADYMTGSSEQVLINSASTQ TSNESAPQSSPVGRWRFCINQTIKNREAQSPPSLQPSVAMVPGLHPFPNNTSNQEISQDTLFTVPGHHVV FTSKLDGKIGERASVETEQTSVPYQAEDDKLKAPATDTSNYSATLVCPDPAGCEALTSQAGMFIPTYPCQ QAAVPADVLMSHPGESVQIGSNAVVTSVLVSSDQKPQSLSVQQPTIDAEFISQEGETTVNSETSSPKAVG ATQTPGFEPTVLLPATILESDGERPPKMEFTDNRIKTLDEKLRNLLYQEHSISTICPESQKDTQSIDSPF SSSAEDILSSPMPEVIAISHCGIQDSQAQSPNFQPTGSKILSNVAASQPAHIPVLKRDLNVITSVPSELC LHEMSPDASLPGDPEAYPAAVSSDGTIHLQTGGGYFGLSFTCPSLKNPISRKSWTRKLKSWAYRLRQSTS FFKRSKVRQVETEDKRSAIASDPIPLTREFSADTRALSRCKAMSGSFQRGRFQVITVPQQQPVKMMSFGK EHRPPSKKTTAQSNEQALTFTEAAVTQLIEVEPAIPTHKASVSSQKLRTLYETFKEDKGDPEQGDILSLS TARETSVSSVTTEKNVEETSTTGISVQSGSEMLDKEKDESTPGKQTCTNEFSATPAGSGKSVAKAAPKSG QHLPARAQTQSSLFYSPSSPMSSDNESEIEDEDLKAELQRLREKHIQEVVSLQTQQNKELQELYERLRAT KDSKAQSSEVPMPPASPRRPRSFKSKLRSRPQSLTHSDNLVVVKDVQRAESNTAPRQQSPASKKGMFTDD LHKLVDDWTRETVGNFPSKPSLNQLKQSQQKSEAENWNKLHESTPSTVGYTSTWISSLSQIRGAAPSSLP HGLPLPSFPGPLASYGMPHACQYNPVGAASYPVQWVGISGAAQQSVVLPTQSGGLFQPGMNLQSFPAPPV QNPPSIPPGPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001163607
ORF Size	5073 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163607.1, NP_001157079.1
RefSeq Size	5076 bp
RefSeq ORF	5076 bp
Locus ID	317420
MW	185.8 kDa

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