Baz1b (NM_001191916) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217638 representing NM_001191916 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCTGTTGGGCCGCAAGCCCTTTCCTCTGGTGAAACCGCTGCCCGGAGAGGAGCCGCTCTTTA CCATCCCGCACACACAAGAGGCCTTCCGCACTCGGGAAGAGTATGAAGCCCGCTTGGAACGATACAGCGA GCGCATTTGGACCTGTAAGAGCACAGGAAGCAGTCAGCTAACACACAAGGAGGCCTGGGAGGAAGAACAG GAAGTTGCTGAACTTTTGAAGGAAGAGTTCCCTAACTGGTATGAGAAGCTTGTTCTGGAAATGGTTCACC ATAACACAGCCTCCTTAGAGAAGCTTGTAGATTCCGCCTGGTTAGAAATCATGACCAAATATGCTGTGGG AGAAGAGTGTGACTTTGAGGTAGGAAAGGAGAAAATGCTCAAAGTGAAGATTGTGAAGATTCATCCTTTG GAGAAAGTTGATGAAGAGGCTGCTGAGAAGAAATCTGACGGGACCTGTGATTCTCCATCAAGTGACAAGG AGAATTCCAGCCAAATGGCTCAGGACCACCAGAAGGAGACAGTAGTCAAAGAGGACGGAGGCAGGAGAGA GAGTATTAATGACAGAGCACGTAGGTCTCCGAGAAAACTTCCTACTTCGTTAAAAAAAGGAGAAAGGAAA TGGGCTCCTCCAAAATTTCTGCCTCACAAATACGATGTGAAGCTACAGAGTGAGGATAAGATCATCAGCA ATGTGCCTGCAGACAGCTTGATTCGGACAGAGAGACCCCCAAATAAGGAGATACTCCGATACTTCATCCG GCATAACGCCTTACGCGCTGGTACTGGGGAGAATGCGCCTTGGGTGGTAGAAGATGAATTGGTGAAGAAG TATTCCCTGCCCAGCAAGTTCAGTGACTTTTTACTTGACCCATATAAGTATATGACTCTCAATCCCTCTA CCAAGAGGAAGAATACTGGATCTCCAGACAGGAAGCCCTCAAAGAAACCTAAGAGAGACAATTCTTCTCT GAGTTCCCCACTAAACCCTAAGCTGTGGTGTCATGTGCACTTGGAGAAGTCGTTAAATGGCCCGCCACTC AAAGTAAAGAACTCAAAGAATTCCAAGTCTCCAGAAGAACATTTGGAAGAGGTGATGAAAATTATGTCCC CCAACAAGCTACACAGCTTCCACATACCTAAGAAAGGCCCGTCCACCAAGAAGCCGGGGAAGCACAGCGA CAAACCTCTGAAGGCCAAGGGCAGGGGCAAAGGCATCCTGAACGGACAGAAGTCCACAGGCAACTCCAAG TCGCCCAGCAAGTGTGTGAAGACGCCTAAGACCAAAATGAAGCAGATGACTTTGTTGGATATGGCCAAAG GGACTCAGAAGATGACTCGCACCCCGAGGAGTTCTGGGGGTGTGCCAAGGTCTTCTGGCAAACCCCATAA ACACCTACCACCTGCAGCCCTGCATCTGATTGCCTATTACAAAGAAAATAAAGACAAAGAGGACAAGAAG AGCGCCCTGTCCTGTGTAATCTCCAAAACCGCACGGCTTCTCTCCAGTGAAGACAGAGCCCGTCTTCCAG AGGAGCTCCGGGCTCTTGTCCAGAAACGCTATGAGCTCCTGGAACATAAAAAGAGGTGGGCCTCTATGTC TGAAGAGCAACGGAAAGAATATTTGAAAAAGAAACGACAGGAGCTAAAGGAGAGGTTGCGGGAAAAAGCC AAAGAACGGAGAGAGAAAGAAATGCTGGAGAGGTTAGAAAAGCAGAAGCGATATGAGGACCAGGAGTTAG GTGGCAGAAACCTCCCGACATTCAGGCTGGTAGATACCCCTGAGGGGCTGCCCAACACCCTTTTTGGGGA TGTGGCCCTGGTAGTGGAGTTCCTGAGCTGTTACTCCGGGTTGCTCTTGCCTGATGCTCAGTACCCTATC ACTGCCGTGTCCCTAATGGAAGCCTTGAGTGCAGATAAAGGTGGTTTTCTGTACTTGAACAGAGTGCTGG TCATCCTGCTGCAGACCTTACTGCAGGATGAAATTGCAGAAGACTATGGAGAACTGGGCATGAAGCTGTC GGAAATCCCCCTCACCCTGCACTCTGTCTCAGAGCTGGTCCGGCTCTGCTTGCGCAGGTGTGACGTCCAG GAAGACAGCGAGGGCTCAGACACAGATGACAATAAAGATTCAACACCATTTGAGGACAACGAGGTACAAG ACGAGTTCCTAGAGAAGTTGGAGACATCAGAATTCTTTGAGCTGACCTCAGAGGAGAAGCTACGGATATT GACAGCTCTGTGCCACCGGATCCTCATGACGTACTCTGTGCAAGACCACATGGAGACCAGACAGCAGATG TCTGCAGAGTTGTGGAAGGAGCGGCTTGCCGTGCTGAAGGAGGAGAATGATAAGAAGAGAGCAGAGAAAC AAAAGCGCAAGGAGATGGAAGCCAGAAATAAGGAAAATGGCAAAGAGGAGAACGTGCTAGGCAAAGTGGA CAGGAAGAAGGAAATTGTGAAGATTGAAAACCAAGTGGAAGTGGAAGCAGATGACATGATTAGCGCTGTC AAGAGCAGAAGGCTGCTTAGCATGCAAGCTAAGAGGAAGCGGGAAATCCAGGAGAGAGAAACAAAAGTGA GATTGGAACGGGAAGCTGAAGAGGAGCGAGTGCGGAAGCACAAGGCTGCGGCAGAGAAGGCTTTCCAGGA AGGGATTGCCAAGGCAAAGCTCGTCCTGCGCAGGACACCTATTGGGACAGACCGCAACCACAACCGATAC TGGCTCTTCTCAAACGAGGTCCCAGGGTTATTCATTGAGAAGGGCTGGGTCCATAACAGCATCGACTACC GGTTTAAGCATCACCGCAAAGACCACAACAATCTACCTGATGATGATTACTGTCCCCGCAGTAAGAAAGC AAACCTAGGCAAAAACGCAAGTGTGAATGCACACCTTGGATCAGCACTAGAAGCTGTGGAGACCACTGTG CCCAAGCAAGGGCAGAACCTATGGTTTCTGTGTGACAGTCAGAAGGAACTGGATGAGTTGCTCAATTGCC TGCATCCTCAGGGAATAAGGGAGAGTCAGCTGAAAGAGAGACTGGAGAAACGGTACCAAGAAATTACACA CTCCATTCATCTGGCTCGGAAGCCAAATTTGGGTCTAAAATCTTGTGACGGCAACCAGGAGCTCTTAAAC TTCCTGAGGAGTGATCTCATCGAAGTGGCGACGAGGCTGCAGAAAGGAGGACTTGGCTACATGGAGGGAA CATCCGAGTTTGAAGCCCGGGTCATCTCCTTAGAGAAACTGAAGGATTTTGGTGAATGCGTCATCGCCCT TCAAGCCAGTGTCATAAAGAAATTTCTGCAAGGTTTCATGGCTCCCAAACAGAAGAAACGGAAACTCCAA AGTGAAGACTCCACGAAGTCCGAGGAAGTAGATGAAGAGAAGAAAATGGTAGAGGAAGCAAAGGTTGCCT CTGCCTTGGAGAAATGGAAAACCGCCATCCGTGAAGCACAGACCTTCTCCAGAATGCACGTCCTCCTTGG GATGCTGGATGCGTGTATCAAGTGGGATATGTCTGCAGAAAACGCCAGATGCAAGGTTTGCCGGAAGAAA GGTGAGGATGACAAGCTGATCCTGTGTGACGAGTGTAATAAAGCCTTCCACCTGTTCTGTCTGAGGCCGG CCCTCTATGAAGTACCAGATGGTGAGTGGCAGTGTCCAGCTTGCCAGCCTCCTACTGCCAGGCGCAACTC CCGTGGCAGGAATTACACTGAAGAGTCTACCTCTGAGGACAGCGAAGGTGATGAGAGTGGTGATGAGGAG GAAGAGGAGGAGGAGGAGGAGGAGGAAGAAGAGGATTATGAAGTGGCCGGCTTGCGGTTGAGACCCCGGA AGACCATTCGAGGCAAGCAGAGTGTCATCCCTGCAGCAAGGCCAGGCCGACCGCCGGGTAAGAAGTCACA TGCTGCTAGACGGTCACGGCCAAAGGATGACACAGAGGTCGATGAGCTGGTACTTCAGACCAAGCGTAGC TCAAGGAGGCAGAGTCTGGAGCTACAAAAGTGTGAGGAAATCCTTCACAAATTAGTAAAGTACCGCTTCA GCTGGCCCTTCCGGGAGCCTGTGACCAGAGACGAGGCTGAGGACTACTATGACGTGATCGACCACCCCAT GGACTTCCAGACCATGCAGAACAAATGCTCCTGCGGGAACTATCGGTCTGTTCAGGAGTTCCTCACCGAC GTGAAGCAAGTGTTTGCCAATGCTGAGCTCTACAACTGCCGCGGCAGCCACGTGCTGAGTTGCATGGAGA AGACAGAGCAGTGCCTGCTGGCCCTGCTGCAAAAACACCTCCCCGGCCACCCCTACGTCCGCAGGAAACG CAGGAAGTTTCCTGATAGGCTCGCTGATGATGAAGGGGACAGTGATTCAGAGTCTGTCGGACAGTCCAGG GGACGAAGACAGAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191916
Insert Size	4431 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191916.1, NP_001178845.1
RefSeq Size	6387 bp
RefSeq ORF	4431 bp
Locus ID	368002

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