Aatk (NM_001168703) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217603 representing NM_001168703 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCGCGCTGCTGGCCCTGGCCATGTCGTCGTCCTTCTTCAACCCCAGCTTTGCCTTCAGCTCCC ACTTCGACCCGGACGGTGCCCCGCTCAGTGAGCTATCCTGGTCCTCGTCCCTTGCAGTGGTTGCTGTGTC CTTCTCTGGGATCTTCACTGTCATTGTCCTCATGCTGGCCTGCCTGTGCTGTAAGAAGGGCGGCATTGGG TTCAAGGAGTTTGAGAATGCTGAAGGGGAAGAGTATGTGGCCGACTTCTCGGAGCAGGGCTCCCCGGCTG CTACTGTACAGAACGGCCCCGATGTGTATGTCCTGCCTCTCACTGAGGTCTCCCTGCCCATGGCTAAGCA GCCGGGTCGCTCAGTGCAACTCCTCAAGTCCACGGACCTGGGCCGCCACAGCCTCCTGTACTTGAAGGAG ATTGGCCACGGTTGGTTTGGGAAGGTGTTCTTGGGGGAGGTGCACTCGGGTGTCAGTGGCACGCAGGTGG TGGTGAAGGAGCTGAAGGCCAGCGCCAGTGTGCAGGAGCAGATGCAGTTCCTGGAGGAGGCGCAGCCCTA CAGGGCCCTGCAGCACAGCAACCTGCTTCAGTGCCTGGCCCAGTGTGCTGAGGTGACCCCCTATTTGCTG GTTATGGAGTTCTGTCCCCTGGGGGACCTCAAAGGTTATCTACGGAGCTGCCGGGTGACAGAGTCCATGG CGCCTGACCCCCTTACCTTGCAGCGCATGGCGTGCGAGGTGGCGTGTGGTGTCTTGCATCTACATCGTCA CAACTATGTGCACAGTGACCTGGCCCTGAGGAACTGCCTGCTGACGGCTGACCTGACAGTGAAGGTTGGC GACTATGGCCTGGCCCATTGCAAATACAGGGAAGACTACCTCGTGACGGCCGACCAGTTGTGGGTGCCGC TGCGTTGGATCGCGCCAGAGCTGGTGGATGAGGTGCATGGCAACCTACTGGTGGTGGACCAGACTAAGAC CAGCAATGTGTGGTCCCTGGGTGTGACCATCTGGGAGCTTTTCGAGTTGGGCGCGCAGCCCTACCCCCAG CACTCGGACCGGCAGGTGCTGGCTTACGCCGTCCGAGAACAGCAACTTAAGTTGCCCAAGCCCCAGCTAC AGCTGACTCTGTCTGATCGATGGTACGAGGTGATGCAGTTCTGCTGGCTTCAGCCAGAACAGAGGCCTAC GGCTGAGGAGGTTCATCTGCTGCTGTCCTACTTGTGCGCCAAGGGCACCACGGAGCTGGAGGAGGAGTTC GAGCGGCGCTGGCGCTCCCTGCGGCCGGGGGGCAGCGCGGGGCTGGGCTCAGCTTCCACAGTCCCGGCAG CTGCCGCATCTGAACTCACTGCTGCTTCATCTTTCCCGCTGCTGGAGCAGTTCACCAGTGACGGCTTCCA CGTGGACAGCGACGACGTGCTGACAGTCACTGAGACGAGCCACGGCCTCAACTTCGAATACAAGTGGGAG GCTGGCTGTGGCGCTGAGGCTTACCCGCCCCCAGGCGCTGCTTTCAGCCCAGGTTCCGCAGGGCGCCTGC AGGAGCTGTGTGCACCCGACAGCTCGCCGCCGGGTGTGGTGCCGGTCCTCAGTGCCCACAGCCCCTCAGT GGGTAGCGAGTACTTCATCCGCCTGGAGGGGGCAGTGCCGGCTGCTGGCCATGACCCAGACTGTGCCGGC TGTGCTCCCAGCCCCCAAGCTGTGTCCGAGCAGGACAATAACTCTGAGGAGAGCACCGCTGCATCCCTTG TCATGGAGCCGCTTCTGGGCCACGCACCACCCACTGGGGGCCTGTGGGGCCCCTGCGACCATCATTCTCG AAGGAGGCAAGAGCCACCCTGCCCCTCACGCTCACCCTCTCCTGGGACCCCGATGTTGCCAGCTGAAGAC ATAGACTGGGGTGTAGCTACCTTCTGCCCACCCTTTTTTGATGACCCACTGGGCACATCTCCCTCTGGGA GTCCTGGGGCCCAGCCATCCCCCAGTGATGAGGAGCTGGAGGAGGGAAAGACTGGGAGGGCTGCTCAGTG TGGACACTGGAGCTCTAACATGTCTGCCAACAATAACAGTGGCAGTCGAGACCCAGAATCCTGGGATCCT GGCTATGTGAGCAGCTTCACAGACAGCTACAGGGACGACTGCTCCAGCTTAGAGCAGACCCCTCGGGCCT CCCCTGAGCTGGGCCATCCCCTGTCCCAGGAGGATTCCAGAGATTTTTTACCTGGGCTAGTAGCAGCTTC CCCTGGCCAGGAGTCAAGCCGTTGCTTCAACCTGCTCCCTCTGTGTCCTGCCAAAGGCCTGGCACCTGCT GCTTGCCTCGTCCCACCCCCCTGGACAGAGGCAGCTGTAGGTGGGGCTGAGAACCCCATTGTCGAACCCA AACTTGCCCAGGAGGCTGAGGGCTCTGCTGAACCCCAGCTACCCCTTCCTTCTGTCCCCTCCCCATCCCA CGAAGGAGCCTCGCTTCCCTCGGAGGAGGCAAGCGCTCCTGACATCCAGCCTGCCTCTCCTACACCCGCT GCTGGCAGCTGGGTGACCGTCCCTGAGCCAGCCCCCACCCTGGATAGCAGCAGCAGTTCTCTGGGGCAAG AGGTACCCAGCAGCGAGGATGAAGACACAACCGAGGTTACGTCAGGAGTCTTCACCGACCTGTCCAGCGA CGGCCCACACACGGAGAAGTCAGGCATAGTACCAGCCTTGCGCTGTCTGCAGAAGCAGGTGGGGACCCCT GACTCCCTGGACTCTTTGGACATCCCGTCCTCAGCCAGCGATGGCGGCTGTGAGGTCTTAAGCCCATCAG CTGCTGGCCCCGCTGGTGGGCAGCCCCGTGCCATGGACAGTGGCTATGATACAGAGAACTATGAGTCCCC TGAGTTTGTGCTCAAGGAGGCCCATGAGTCAAGTGAGCCTGAGGCCTTTGGGGAGCCAGCCTCAGAGGGT GAGAGCCCAGGGCCCGATACTCTGCTCTCTGTCTCCCTTGGTGACCTCAGCAGAAAGAACCCCTACCGAG ACTCTGCCTACTTTTCAGACCTGGATGCTGAGTCCGAACCCAACTTTGGCCCTGAGAAGCACAGTGGGAT CCAGGACTCCCAAAAGGAGCAAGACCTGAGGAGCCCACCTAGTCCAGGGCATGAGTCTGTGCAGGCTTTT CCCAGGCCTGAGGTGTCCAGCGAGGTGCTGCCCCCTCCACAGCAGTCAGAGGAGCCCATGCCAGAAGGCC CCGGGTCAGAGCCTCTTGAGGCTCAAGGCCCAGTTGAAGTGGAGCCCGTGCCAGGCCCAAGTCATTCCAA ATGTCTCCTGTTGACCTCCGCTCCACTGAGCTCGGAAGGCAATGGCACGGAGTCCCGGGGTCCCCCAGGA CCACTGTCAGGGCCAGCCCAGACCGGGCGGATGGGGAATCCTAGCACATCCAGATCCCCGCTCTGCCTGG CCCTGCCTGGCCACCCTGGGACTTCAGAGGGACGGCCAGAGGATGACGAGGACACCGAGGACAGTGAGGA GTCTGACGAGGAGCTCCGGTGCTACAGCGTCCAGGAGCCCAGTGAGGACAGTGAGGAGGAGCCATCAGCG GTGCCTGTGGTAGTGGCTGAGAGCCAGAGTGCCCGGAATCTACGCAGCCTGCTGAAGATGCCCAGCCTGC TGTCGGAGGCCTTCTGCGACGACCTGGAGCGCAAGAAGAAGGCTGTGTCCTTCTTTGATGACGTCACGGT CTACCTCTTTGACCAGGAGAGCCCCACCCGAGAAGCTGGGGAGCCCTTCCCCAGCACGAAGGAGTGTCCC ACGTTCCTGGAGGGTAGCCCCGGCTCACCCAGTGCCACTGGCGTGCCACTGCGGGCTGGTCACTCGCCAG ACAGCTCTGCTCCCGAACCGGGCAGTAGGTTCGAATGGGATGGAGATTTCCCACTGGTGCCGGGCAAGGC TGCTTTGGTGACTGCGCTGGATCCTGCCGACCCTGTCCTGGCTGCGCCTCCCACGCCAGCTGCACCATTT TCACGCTTCACGGTGTCGCCCGCACCTCCCTCCCGCTTTTCCATCACACAAGTATCCGACTCAGATGCCC AGTCCATGGGAGGCCCGGCAGCAGATGCTGAGGGCCGATGCACAAAGGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001168703
Insert Size	4113 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001168703.1, NP_001162174.1
RefSeq Size	5297 bp
RefSeq ORF	4113 bp
Locus ID	690853

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