Aatk (NM_001168703) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217603 representing NM_001168703 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCATCGCGCTGCTGGCCCTGGCCATGTCGTCGTCCTTCTTCAACCCCAGCTTTGCCTTCAGCTCCC ACTTCGACCCGGACGGTGCCCCGCTCAGTGAGCTATCCTGGTCCTCGTCCCTTGCAGTGGTTGCTGTGTC CTTCTCTGGGATCTTCACTGTCATTGTCCTCATGCTGGCCTGCCTGTGCTGTAAGAAGGGCGGCATTGGG TTCAAGGAGTTTGAGAATGCTGAAGGGGAAGAGTATGTGGCCGACTTCTCGGAGCAGGGCTCCCCGGCTG CTACTGTACAGAACGGCCCCGATGTGTATGTCCTGCCTCTCACTGAGGTCTCCCTGCCCATGGCTAAGCA GCCGGGTCGCTCAGTGCAACTCCTCAAGTCCACGGACCTGGGCCGCCACAGCCTCCTGTACTTGAAGGAG ATTGGCCACGGTTGGTTTGGGAAGGTGTTCTTGGGGGAGGTGCACTCGGGTGTCAGTGGCACGCAGGTGG TGGTGAAGGAGCTGAAGGCCAGCGCCAGTGTGCAGGAGCAGATGCAGTTCCTGGAGGAGGCGCAGCCCTA CAGGGCCCTGCAGCACAGCAACCTGCTTCAGTGCCTGGCCCAGTGTGCTGAGGTGACCCCCTATTTGCTG GTTATGGAGTTCTGTCCCCTGGGGGACCTCAAAGGTTATCTACGGAGCTGCCGGGTGACAGAGTCCATGG CGCCTGACCCCCTTACCTTGCAGCGCATGGCGTGCGAGGTGGCGTGTGGTGTCTTGCATCTACATCGTCA CAACTATGTGCACAGTGACCTGGCCCTGAGGAACTGCCTGCTGACGGCTGACCTGACAGTGAAGGTTGGC GACTATGGCCTGGCCCATTGCAAATACAGGGAAGACTACCTCGTGACGGCCGACCAGTTGTGGGTGCCGC TGCGTTGGATCGCGCCAGAGCTGGTGGATGAGGTGCATGGCAACCTACTGGTGGTGGACCAGACTAAGAC CAGCAATGTGTGGTCCCTGGGTGTGACCATCTGGGAGCTTTTCGAGTTGGGCGCGCAGCCCTACCCCCAG CACTCGGACCGGCAGGTGCTGGCTTACGCCGTCCGAGAACAGCAACTTAAGTTGCCCAAGCCCCAGCTAC AGCTGACTCTGTCTGATCGATGGTACGAGGTGATGCAGTTCTGCTGGCTTCAGCCAGAACAGAGGCCTAC GGCTGAGGAGGTTCATCTGCTGCTGTCCTACTTGTGCGCCAAGGGCACCACGGAGCTGGAGGAGGAGTTC GAGCGGCGCTGGCGCTCCCTGCGGCCGGGGGGCAGCGCGGGGCTGGGCTCAGCTTCCACAGTCCCGGCAG CTGCCGCATCTGAACTCACTGCTGCTTCATCTTTCCCGCTGCTGGAGCAGTTCACCAGTGACGGCTTCCA CGTGGACAGCGACGACGTGCTGACAGTCACTGAGACGAGCCACGGCCTCAACTTCGAATACAAGTGGGAG GCTGGCTGTGGCGCTGAGGCTTACCCGCCCCCAGGCGCTGCTTTCAGCCCAGGTTCCGCAGGGCGCCTGC AGGAGCTGTGTGCACCCGACAGCTCGCCGCCGGGTGTGGTGCCGGTCCTCAGTGCCCACAGCCCCTCAGT GGGTAGCGAGTACTTCATCCGCCTGGAGGGGGCAGTGCCGGCTGCTGGCCATGACCCAGACTGTGCCGGC TGTGCTCCCAGCCCCCAAGCTGTGTCCGAGCAGGACAATAACTCTGAGGAGAGCACCGCTGCATCCCTTG TCATGGAGCCGCTTCTGGGCCACGCACCACCCACTGGGGGCCTGTGGGGCCCCTGCGACCATCATTCTCG AAGGAGGCAAGAGCCACCCTGCCCCTCACGCTCACCCTCTCCTGGGACCCCGATGTTGCCAGCTGAAGAC ATAGACTGGGGTGTAGCTACCTTCTGCCCACCCTTTTTTGATGACCCACTGGGCACATCTCCCTCTGGGA GTCCTGGGGCCCAGCCATCCCCCAGTGATGAGGAGCTGGAGGAGGGAAAGACTGGGAGGGCTGCTCAGTG TGGACACTGGAGCTCTAACATGTCTGCCAACAATAACAGTGGCAGTCGAGACCCAGAATCCTGGGATCCT GGCTATGTGAGCAGCTTCACAGACAGCTACAGGGACGACTGCTCCAGCTTAGAGCAGACCCCTCGGGCCT CCCCTGAGCTGGGCCATCCCCTGTCCCAGGAGGATTCCAGAGATTTTTTACCTGGGCTAGTAGCAGCTTC CCCTGGCCAGGAGTCAAGCCGTTGCTTCAACCTGCTCCCTCTGTGTCCTGCCAAAGGCCTGGCACCTGCT GCTTGCCTCGTCCCACCCCCCTGGACAGAGGCAGCTGTAGGTGGGGCTGAGAACCCCATTGTCGAACCCA AACTTGCCCAGGAGGCTGAGGGCTCTGCTGAACCCCAGCTACCCCTTCCTTCTGTCCCCTCCCCATCCCA CGAAGGAGCCTCGCTTCCCTCGGAGGAGGCAAGCGCTCCTGACATCCAGCCTGCCTCTCCTACACCCGCT GCTGGCAGCTGGGTGACCGTCCCTGAGCCAGCCCCCACCCTGGATAGCAGCAGCAGTTCTCTGGGGCAAG AGGTACCCAGCAGCGAGGATGAAGACACAACCGAGGTTACGTCAGGAGTCTTCACCGACCTGTCCAGCGA CGGCCCACACACGGAGAAGTCAGGCATAGTACCAGCCTTGCGCTGTCTGCAGAAGCAGGTGGGGACCCCT GACTCCCTGGACTCTTTGGACATCCCGTCCTCAGCCAGCGATGGCGGCTGTGAGGTCTTAAGCCCATCAG CTGCTGGCCCCGCTGGTGGGCAGCCCCGTGCCATGGACAGTGGCTATGATACAGAGAACTATGAGTCCCC TGAGTTTGTGCTCAAGGAGGCCCATGAGTCAAGTGAGCCTGAGGCCTTTGGGGAGCCAGCCTCAGAGGGT GAGAGCCCAGGGCCCGATACTCTGCTCTCTGTCTCCCTTGGTGACCTCAGCAGAAAGAACCCCTACCGAG ACTCTGCCTACTTTTCAGACCTGGATGCTGAGTCCGAACCCAACTTTGGCCCTGAGAAGCACAGTGGGAT CCAGGACTCCCAAAAGGAGCAAGACCTGAGGAGCCCACCTAGTCCAGGGCATGAGTCTGTGCAGGCTTTT CCCAGGCCTGAGGTGTCCAGCGAGGTGCTGCCCCCTCCACAGCAGTCAGAGGAGCCCATGCCAGAAGGCC CCGGGTCAGAGCCTCTTGAGGCTCAAGGCCCAGTTGAAGTGGAGCCCGTGCCAGGCCCAAGTCATTCCAA ATGTCTCCTGTTGACCTCCGCTCCACTGAGCTCGGAAGGCAATGGCACGGAGTCCCGGGGTCCCCCAGGA CCACTGTCAGGGCCAGCCCAGACCGGGCGGATGGGGAATCCTAGCACATCCAGATCCCCGCTCTGCCTGG CCCTGCCTGGCCACCCTGGGACTTCAGAGGGACGGCCAGAGGATGACGAGGACACCGAGGACAGTGAGGA GTCTGACGAGGAGCTCCGGTGCTACAGCGTCCAGGAGCCCAGTGAGGACAGTGAGGAGGAGCCATCAGCG GTGCCTGTGGTAGTGGCTGAGAGCCAGAGTGCCCGGAATCTACGCAGCCTGCTGAAGATGCCCAGCCTGC TGTCGGAGGCCTTCTGCGACGACCTGGAGCGCAAGAAGAAGGCTGTGTCCTTCTTTGATGACGTCACGGT CTACCTCTTTGACCAGGAGAGCCCCACCCGAGAAGCTGGGGAGCCCTTCCCCAGCACGAAGGAGTGTCCC ACGTTCCTGGAGGGTAGCCCCGGCTCACCCAGTGCCACTGGCGTGCCACTGCGGGCTGGTCACTCGCCAG ACAGCTCTGCTCCCGAACCGGGCAGTAGGTTCGAATGGGATGGAGATTTCCCACTGGTGCCGGGCAAGGC TGCTTTGGTGACTGCGCTGGATCCTGCCGACCCTGTCCTGGCTGCGCCTCCCACGCCAGCTGCACCATTT TCACGCTTCACGGTGTCGCCCGCACCTCCCTCCCGCTTTTCCATCACACAAGTATCCGACTCAGATGCCC AGTCCATGGGAGGCCCGGCAGCAGATGCTGAGGGCCGATGCACAAAGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217603 representing NM_001168703 Red=Cloning site Green=Tags(s) MPIALLALAMSSSFFNPSFAFSSHFDPDGAPLSELSWSSSLAVVAVSFSGIFTVIVLMLACLCCKKGGIG FKEFENAEGEEYVADFSEQGSPAATVQNGPDVYVLPLTEVSLPMAKQPGRSVQLLKSTDLGRHSLLYLKE IGHGWFGKVFLGEVHSGVSGTQVVVKELKASASVQEQMQFLEEAQPYRALQHSNLLQCLAQCAEVTPYLL VMEFCPLGDLKGYLRSCRVTESMAPDPLTLQRMACEVACGVLHLHRHNYVHSDLALRNCLLTADLTVKVG DYGLAHCKYREDYLVTADQLWVPLRWIAPELVDEVHGNLLVVDQTKTSNVWSLGVTIWELFELGAQPYPQ HSDRQVLAYAVREQQLKLPKPQLQLTLSDRWYEVMQFCWLQPEQRPTAEEVHLLLSYLCAKGTTELEEEF ERRWRSLRPGGSAGLGSASTVPAAAASELTAASSFPLLEQFTSDGFHVDSDDVLTVTETSHGLNFEYKWE AGCGAEAYPPPGAAFSPGSAGRLQELCAPDSSPPGVVPVLSAHSPSVGSEYFIRLEGAVPAAGHDPDCAG CAPSPQAVSEQDNNSEESTAASLVMEPLLGHAPPTGGLWGPCDHHSRRRQEPPCPSRSPSPGTPMLPAED IDWGVATFCPPFFDDPLGTSPSGSPGAQPSPSDEELEEGKTGRAAQCGHWSSNMSANNNSGSRDPESWDP GYVSSFTDSYRDDCSSLEQTPRASPELGHPLSQEDSRDFLPGLVAASPGQESSRCFNLLPLCPAKGLAPA ACLVPPPWTEAAVGGAENPIVEPKLAQEAEGSAEPQLPLPSVPSPSHEGASLPSEEASAPDIQPASPTPA AGSWVTVPEPAPTLDSSSSSLGQEVPSSEDEDTTEVTSGVFTDLSSDGPHTEKSGIVPALRCLQKQVGTP DSLDSLDIPSSASDGGCEVLSPSAAGPAGGQPRAMDSGYDTENYESPEFVLKEAHESSEPEAFGEPASEG ESPGPDTLLSVSLGDLSRKNPYRDSAYFSDLDAESEPNFGPEKHSGIQDSQKEQDLRSPPSPGHESVQAF PRPEVSSEVLPPPQQSEEPMPEGPGSEPLEAQGPVEVEPVPGPSHSKCLLLTSAPLSSEGNGTESRGPPG PLSGPAQTGRMGNPSTSRSPLCLALPGHPGTSEGRPEDDEDTEDSEESDEELRCYSVQEPSEDSEEEPSA VPVVVAESQSARNLRSLLKMPSLLSEAFCDDLERKKKAVSFFDDVTVYLFDQESPTREAGEPFPSTKECP TFLEGSPGSPSATGVPLRAGHSPDSSAPEPGSRFEWDGDFPLVPGKAALVTALDPADPVLAAPPTPAAPF SRFTVSPAPPSRFSITQVSDSDAQSMGGPAADAEGRCTKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001168703
ORF Size	4110 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001168703.1, NP_001162174.1
RefSeq Size	5297 bp
RefSeq ORF	4113 bp
Locus ID	690853
MW	145.5 kDa

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