Astn2 (NM_001271363) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217599 representing NM_001271363 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCCGGCGCCCGGCGCAGCCCGGGCCCCGGCTTGGGGCTTCGGGGGCGGCCGAGGCTCCGCT TCCACCCGGGGCCGCCGCCGCCACCGCCACTGCCGCTGCTGTTGCTGTTTCTGCTCTTGCTGCCGCCGCC GCCGTTGCTGGCCGGCGCCACCGCCGCCGCCGCCTCGCGGGAGCCCGACAGCCCATGCCGGCTCAAGACT GTCACGGTATCCACGCTGCCCGCTCTGCGGGAAAGCGACATCGGCTGGAGCGGTGCCCGCGCCGGGGCCG CGGCCGGGGCTGGGGCCGGGACCGGAGCCGGGGCCGGAGCTTCTGCTGCTGCCGCCGCTGCTGCCGCCGC GTCCCCGGGCTCCGCGGGTTCAGCCGGTACAGCCGCGGAGTCTCGTCTCCTGCTCTTTGTGCGTAACGAG CTCCCTGGGCGCATCGCGGTACAAGATGACCTGGACAACACCGAGCTGCCCTTCTTCACCCTGGAGATGT CTGGCACAGCAGCGGACATTTCGTTGGTTCACTGGAGACAGCAGTGGCTGGAGAATGGCACACTGTACTT CCATGTTTCCATGAGCAGCTCTGGGCAGCTGGCCCAGGCCACTGCTCCCACACTGCAGGAGCCCTCAGAA ATCGTTGAAGAACAGCTGCATATCCTCCACATCTCTGTGATGGGTGGACTCATTGCACTGCTCCTTTTGC TCCTGGTGTTCACAGTGGCATTGTATGCCCAACGGCGCTGGCAGAAGCGCAGGCGCATCCCACAGAAGAG CGCAAGCACAGAAGCCACTCATGAGATTCACTACATCCCATCTGTGCTGCTCGGTCCTCAGGCCAGGGAG AGCTTCCGCTCCACCAGACTACAGGCACACAACTCAGTCATCGGTGTGCCTATCCGGGAAACCCCCATCC TAGATGACTATGACTATGAAGAGGAAGAGGACCCACCCAGGCGGGCCAACCATGTCTCCCGTGAGGATGA GTTTGATAGCCAGATGACCCATGCCCTGGACAGCTTGGGAAGGCCAGGAGAAGAGAAAGTGGAATTTGAG AAGAAAGCAGCAGCTGAGGCGACACAGGAGACAGTGGAGTCCCTGATGCAGAAGTTCAAGGAGAGTTTCC GAGCTAACACACCTGTGGAGATCGGTCAATTGCAGCCAGCCTCGCGCAGCAGCACCTCTGCAGGGAAGAG GAAACGGAGGAACAAGTCTCGAGGGGGAATCAGTTTTGGGAGAACCAAGGGAACATCAGGCTCAGAAGCA GATGACGAAACACAGCTGACGTTCTACACAGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTACTGA AAAGCCCTGTGAACAAGACAGCCTTAACACTGATTGCTGTCAGCTCCTGCATTTTGGCCATGGTGTGTGG CAACCAGATGTCCTGTCCACTTACTGTGAAGGTGACTTTGCATGTGCCGGAGCACTTCATTGCAGATGGA AGCAGCTTTGTGGTGAGTGAAGGAAGCTACCTGGATATCTCTGACTGGCTAAACCCTGCCAAGCTGTCCT TGTACTACCAGATCAATGCCACCTCACCATGGGTGAGAGACCTCTGTGGACAGAGGACGACAGATGCCTG TGAACAGCTTTGTGACCCAGAAACTGGGGAGTGCAGTTGTCACGAAGGTTACGCCCCTGACCCAGTCCAC AGACATCTATGTATCCGAAGTGACTGGGGTCAAAGTGAAGGACCTTGGCCTTACACAACACTGGAGAGGG GTTATGATCTGGTGACAGGAGAGCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAGCTTGGGCCAAGG CCTCTGGCTTCCGGTCAGCAAAAGCTTCGTGGTTCCACCGGTGGAGCTGTCCATCAACCCCCTGGCAAGT TGCAAGACTGATGTACTCGTCACAGAAGACCCTGCAGATGTCAGGGAAGAAGCAATGCTGTCCACGTACT TTGAGACCATCAATGACCTGCTCTCCTCCTTTGGTCCAGTCCGTGACTGCTCAAGGAACAATGGGGGCTG CACCCGCAACTTCAAGTGTGTGTCTGACCGCCAGGTGGACTCCTCGGGATGTGTGTGTCCTGAGGAACTG AAGCCCATGAAGGATGGTTCCGGCTGCTATGATCACTCCAAGGGCATTGACTGCTCTGATGGCTTTAATG GAGGGTGTGAGCAGCTTTGCCTACAACAAACAATGCCTTTGCCCTATGATGCCACCTCCAGCACCATCTT CATGTTCTGCGGCTGTGTGGAGGAGTACAAGCTGGCTCCTGATGGGAAATCCTGTCTAATGCTCTCGGAT GTCTGTGACGGCCCCAAGTGCCTCAAATCTGACTCCAAATTCAATGATACCCTCTTTGGAGAGATGCTAC ATGGTTACAACAACCGGACACAACATGTAAACCAAGGCCAAGTCTTCCAAATGACATTCAGGGAGAACAA CTTCATCAAGGACTTTCCCCAGCTGGCGGATGGACTGTTGGTGATCCCGCTACCAGTGGAAGAGCAATGC CGAGGGGTTCTCTCTGAGCCCCTCCCAGACCTTCAGCTTCTCACGGGAGACATCAGGTATGACGAGGCCA TGGGTTACCCCATGGTGCAGCAGTGGCGTGTCCGGAGCAACCTCTATCGTGTGAAGCTGAGTACCATCAC CCTCTCAGCAGGCTTCACGAATGTCCTAAAGATCCTGACCAAAGAGAGCAGTCGGGATGAGCTGCTCTCC TTCATCCAGCACTATGGCTCCCACTACATTGCAGAGGCTCTGTATGGTTCTGAACTCACTTGCATCATCC ATTTTCCCAGCAAGAAGGTCCAGCAGCAGCTATGGCTCCAGTATCAGAAAGAGACCACGGAGCTGGGCAG CAAGAAGGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCTGGTCTACTGACAGCCCAGATGCTATCA GATGACCAACTCATCTCAGGTGTGGAGATTCGATGTGAGGAAAAGGGCCGCTGTCCATCTACCTGTCACC TTTGCCGCCGGCCAGGCAAAGAACAGCTGAGCCCCACACCAGTGCTGCTGGAGATTAACCGTGTGGTGCC ACTTTACACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAATGCACTGATGAGTTCTTACTGG TGTTCTGGTAAAGGAGATGTGATCGATGACTGGTGCAGGTGTGACCTCAGTGCCTTCGATGCCAGTGGAC TTCCCAATTGTAGCCCCCTTCCACAGCCGGTGTTACGACTTTCTCCAACAGTGGAACCTTCCAGCACTGT GGTTTCCCTGGAGTGGATAGATGTTCAGCCAGCCATTGGAACCAAAGTTTCTGACTATATCCTGCAGCAC AAGAAAGTGGATGAATACACAGACACTGACCTCTACACAGGAGAATTCCTGAGTTTTGCTGATGACTTAC TCTCTGGCCTGGGCACATCTTGTGTAGCTGCTGGTCGAAGCCACGGAGAGGTCCCGGAAGTCAGTATCTA CTCGGTGATCTTCAAGTGCCTGGAGCCAGATGGCCTTTACAAGTTCACTCTGTATGCCGTGGATACTCGA GGGAGGCACTCAGAGCTCAGCACAGTGACTCTAAGAACAGCTTGTCCACTGGTAGATGACAACAAAGCAG AAGAGATAGCTGACAAGATTTACAATCTATACAATGGTTACACCAGTGGCAAGGAACAGCAGACTGCTTA CAACACACTGATGGAAGTCTCTGCCTCCATGCTCTTCCGAGTCCAGCACCACTACAACTCTCACTATGAA AAGTTTGGTGATTTCGTCTGGAGGAGTGAGGATGAGCTGGGACCGAGGAAGGCTCATTTGATTCTGCGGC GGCTGGAGAGGGTCAGCAGCCATTGTTCTAGTCTCTTGAGAAGCGCCTACATCCAGAGTCGTGTGGATAC CATACCCTATCTCTTCTGCCGCAGTGAGGAGGTCCGGCCTGCAGGAATGGTGTGGTACAGCATCCTCAAA GACACCAAAATCACATGTGAGGAGAAGATGGTGTCCATGGCCCGAAACACGTATGGGGAAACCAAAGGCC GGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271363
Insert Size	4065 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271363.1, NP_001258292.1
RefSeq Size	4229 bp
RefSeq ORF	4065 bp
Locus ID	100361323

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