Astn2 (NM_001271363) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217599 representing NM_001271363 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGCCGGCGCCCGGCGCAGCCCGGGCCCCGGCTTGGGGCTTCGGGGGCGGCCGAGGCTCCGCT TCCACCCGGGGCCGCCGCCGCCACCGCCACTGCCGCTGCTGTTGCTGTTTCTGCTCTTGCTGCCGCCGCC GCCGTTGCTGGCCGGCGCCACCGCCGCCGCCGCCTCGCGGGAGCCCGACAGCCCATGCCGGCTCAAGACT GTCACGGTATCCACGCTGCCCGCTCTGCGGGAAAGCGACATCGGCTGGAGCGGTGCCCGCGCCGGGGCCG CGGCCGGGGCTGGGGCCGGGACCGGAGCCGGGGCCGGAGCTTCTGCTGCTGCCGCCGCTGCTGCCGCCGC GTCCCCGGGCTCCGCGGGTTCAGCCGGTACAGCCGCGGAGTCTCGTCTCCTGCTCTTTGTGCGTAACGAG CTCCCTGGGCGCATCGCGGTACAAGATGACCTGGACAACACCGAGCTGCCCTTCTTCACCCTGGAGATGT CTGGCACAGCAGCGGACATTTCGTTGGTTCACTGGAGACAGCAGTGGCTGGAGAATGGCACACTGTACTT CCATGTTTCCATGAGCAGCTCTGGGCAGCTGGCCCAGGCCACTGCTCCCACACTGCAGGAGCCCTCAGAA ATCGTTGAAGAACAGCTGCATATCCTCCACATCTCTGTGATGGGTGGACTCATTGCACTGCTCCTTTTGC TCCTGGTGTTCACAGTGGCATTGTATGCCCAACGGCGCTGGCAGAAGCGCAGGCGCATCCCACAGAAGAG CGCAAGCACAGAAGCCACTCATGAGATTCACTACATCCCATCTGTGCTGCTCGGTCCTCAGGCCAGGGAG AGCTTCCGCTCCACCAGACTACAGGCACACAACTCAGTCATCGGTGTGCCTATCCGGGAAACCCCCATCC TAGATGACTATGACTATGAAGAGGAAGAGGACCCACCCAGGCGGGCCAACCATGTCTCCCGTGAGGATGA GTTTGATAGCCAGATGACCCATGCCCTGGACAGCTTGGGAAGGCCAGGAGAAGAGAAAGTGGAATTTGAG AAGAAAGCAGCAGCTGAGGCGACACAGGAGACAGTGGAGTCCCTGATGCAGAAGTTCAAGGAGAGTTTCC GAGCTAACACACCTGTGGAGATCGGTCAATTGCAGCCAGCCTCGCGCAGCAGCACCTCTGCAGGGAAGAG GAAACGGAGGAACAAGTCTCGAGGGGGAATCAGTTTTGGGAGAACCAAGGGAACATCAGGCTCAGAAGCA GATGACGAAACACAGCTGACGTTCTACACAGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTACTGA AAAGCCCTGTGAACAAGACAGCCTTAACACTGATTGCTGTCAGCTCCTGCATTTTGGCCATGGTGTGTGG CAACCAGATGTCCTGTCCACTTACTGTGAAGGTGACTTTGCATGTGCCGGAGCACTTCATTGCAGATGGA AGCAGCTTTGTGGTGAGTGAAGGAAGCTACCTGGATATCTCTGACTGGCTAAACCCTGCCAAGCTGTCCT TGTACTACCAGATCAATGCCACCTCACCATGGGTGAGAGACCTCTGTGGACAGAGGACGACAGATGCCTG TGAACAGCTTTGTGACCCAGAAACTGGGGAGTGCAGTTGTCACGAAGGTTACGCCCCTGACCCAGTCCAC AGACATCTATGTATCCGAAGTGACTGGGGTCAAAGTGAAGGACCTTGGCCTTACACAACACTGGAGAGGG GTTATGATCTGGTGACAGGAGAGCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAGCTTGGGCCAAGG CCTCTGGCTTCCGGTCAGCAAAAGCTTCGTGGTTCCACCGGTGGAGCTGTCCATCAACCCCCTGGCAAGT TGCAAGACTGATGTACTCGTCACAGAAGACCCTGCAGATGTCAGGGAAGAAGCAATGCTGTCCACGTACT TTGAGACCATCAATGACCTGCTCTCCTCCTTTGGTCCAGTCCGTGACTGCTCAAGGAACAATGGGGGCTG CACCCGCAACTTCAAGTGTGTGTCTGACCGCCAGGTGGACTCCTCGGGATGTGTGTGTCCTGAGGAACTG AAGCCCATGAAGGATGGTTCCGGCTGCTATGATCACTCCAAGGGCATTGACTGCTCTGATGGCTTTAATG GAGGGTGTGAGCAGCTTTGCCTACAACAAACAATGCCTTTGCCCTATGATGCCACCTCCAGCACCATCTT CATGTTCTGCGGCTGTGTGGAGGAGTACAAGCTGGCTCCTGATGGGAAATCCTGTCTAATGCTCTCGGAT GTCTGTGACGGCCCCAAGTGCCTCAAATCTGACTCCAAATTCAATGATACCCTCTTTGGAGAGATGCTAC ATGGTTACAACAACCGGACACAACATGTAAACCAAGGCCAAGTCTTCCAAATGACATTCAGGGAGAACAA CTTCATCAAGGACTTTCCCCAGCTGGCGGATGGACTGTTGGTGATCCCGCTACCAGTGGAAGAGCAATGC CGAGGGGTTCTCTCTGAGCCCCTCCCAGACCTTCAGCTTCTCACGGGAGACATCAGGTATGACGAGGCCA TGGGTTACCCCATGGTGCAGCAGTGGCGTGTCCGGAGCAACCTCTATCGTGTGAAGCTGAGTACCATCAC CCTCTCAGCAGGCTTCACGAATGTCCTAAAGATCCTGACCAAAGAGAGCAGTCGGGATGAGCTGCTCTCC TTCATCCAGCACTATGGCTCCCACTACATTGCAGAGGCTCTGTATGGTTCTGAACTCACTTGCATCATCC ATTTTCCCAGCAAGAAGGTCCAGCAGCAGCTATGGCTCCAGTATCAGAAAGAGACCACGGAGCTGGGCAG CAAGAAGGAGCTCAAGTCCATGCCCTTCATCACCTACCTCTCTGGTCTACTGACAGCCCAGATGCTATCA GATGACCAACTCATCTCAGGTGTGGAGATTCGATGTGAGGAAAAGGGCCGCTGTCCATCTACCTGTCACC TTTGCCGCCGGCCAGGCAAAGAACAGCTGAGCCCCACACCAGTGCTGCTGGAGATTAACCGTGTGGTGCC ACTTTACACCCTCATCCAAGACAATGGCACAAAGGAGGCCTTCAAGAATGCACTGATGAGTTCTTACTGG TGTTCTGGTAAAGGAGATGTGATCGATGACTGGTGCAGGTGTGACCTCAGTGCCTTCGATGCCAGTGGAC TTCCCAATTGTAGCCCCCTTCCACAGCCGGTGTTACGACTTTCTCCAACAGTGGAACCTTCCAGCACTGT GGTTTCCCTGGAGTGGATAGATGTTCAGCCAGCCATTGGAACCAAAGTTTCTGACTATATCCTGCAGCAC AAGAAAGTGGATGAATACACAGACACTGACCTCTACACAGGAGAATTCCTGAGTTTTGCTGATGACTTAC TCTCTGGCCTGGGCACATCTTGTGTAGCTGCTGGTCGAAGCCACGGAGAGGTCCCGGAAGTCAGTATCTA CTCGGTGATCTTCAAGTGCCTGGAGCCAGATGGCCTTTACAAGTTCACTCTGTATGCCGTGGATACTCGA GGGAGGCACTCAGAGCTCAGCACAGTGACTCTAAGAACAGCTTGTCCACTGGTAGATGACAACAAAGCAG AAGAGATAGCTGACAAGATTTACAATCTATACAATGGTTACACCAGTGGCAAGGAACAGCAGACTGCTTA CAACACACTGATGGAAGTCTCTGCCTCCATGCTCTTCCGAGTCCAGCACCACTACAACTCTCACTATGAA AAGTTTGGTGATTTCGTCTGGAGGAGTGAGGATGAGCTGGGACCGAGGAAGGCTCATTTGATTCTGCGGC GGCTGGAGAGGGTCAGCAGCCATTGTTCTAGTCTCTTGAGAAGCGCCTACATCCAGAGTCGTGTGGATAC CATACCCTATCTCTTCTGCCGCAGTGAGGAGGTCCGGCCTGCAGGAATGGTGTGGTACAGCATCCTCAAA GACACCAAAATCACATGTGAGGAGAAGATGGTGTCCATGGCCCGAAACACGTATGGGGAAACCAAAGGCC GG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217599 representing NM_001271363 Red=Cloning site Green=Tags(s) MAAAGARRSPGPGLGLRGRPRLRFHPGPPPPPPLPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKT VTVSTLPALRESDIGWSGARAGAAAGAGAGTGAGAGASAAAAAAAAASPGSAGSAGTAAESRLLLFVRNE LPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSE IVEEQLHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARE SFRSTRLQAHNSVIGVPIRETPILDDYDYEEEEDPPRRANHVSREDEFDSQMTHALDSLGRPGEEKVEFE KKAAAEATQETVESLMQKFKESFRANTPVEIGQLQPASRSSTSAGKRKRRNKSRGGISFGRTKGTSGSEA DDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPLTVKVTLHVPEHFIADG SSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPETGECSCHEGYAPDPVH RHLCIRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLAS CKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEEL KPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTMPLPYDATSSTIFMFCGCVEEYKLAPDGKSCLMLSD VCDGPKCLKSDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQC RGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSRDELLS FIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLS DDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKNALMSSYW CSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWIDVQPAIGTKVSDYILQH KKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTR GRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYE KFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCRSEEVRPAGMVWYSILK DTKITCEEKMVSMARNTYGETKGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271363
ORF Size	4062 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271363.1, NP_001258292.1
RefSeq Size	4229 bp
RefSeq ORF	4065 bp
Locus ID	100361323
MW	149.6 kDa

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