Col18a1 (NM_053489) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217584 representing NM_053489 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCAGGTGGCACCTCCTGGACATGCTCACCAGTTTGGTCTTGCTGCTGGTGGCACGCGTCTCCT GGGCAGAGCCAGAGAATGTTGCTGAAGATGTGGGGCTCCTGCAGCTCCTCGGAGACCCCCTACCCCAGAA GATCTCCCAAGTCGAAGACCCTCATGTCGGGCTGGCCTACGTCTTTGGACCATACTCCAAAAGTAGTCAG ATGGCCCAGTATCACTTCCCTAAACTCTTCTTCCGGGACTTTTCGCTGCTGTTCGAAGTCCGGCCAACCA CAGAGGCCGCAGGGGTGCTGTTTGCTATCACAGATGCCGCCCAGGTGGTGGTCTCGCTAGGCGTGAAGCT CTCAGAGGTCCGAGATGGACAACAGAACATCTCATTGCTCTACACGGAGCCCGGGGCCAGCCAGACCCAG ACGGGAGCCAGCTTCCGCCTACCTGCGTTTGTTGGCCAGTGGACACACTTCGCGCTCAGCGTGGATGGAA GCTCCGTGGCTCTCTACGTAGACTGTGAAGAGTTCCAGAGAGTGCCGTTCGCTCGGTCCCCACACGGTCT GGAGCTAGAGCGTGGCGCTGGCCTCTTTGTGGGTCAGGCTGGAGCAGCGGACCCTGACAAGTTCCAGGGA ATGATCTCAGAGCTGAGAGTACGCAAAACCCCCCGGGTGAGCCCTGTGCACTGCCTGGATGAAGAAGATG ATGATGACGACCGGGCATCTGGAGATTTTGGAAGTGGCCTCGAAGAAAGCAGCAATTTACACAGGCAGGA GACATATCTACGACCCGGGCTCCCCCAGCCACCTCCTGTCACTTCCCCGCCCTTGGCTGGAGGCAGTGCC ACCGAAGATTCTAGGACAGAAGAAAAGGAGGAAGAAGCCACGGTAGATTCTAAAGGAGCTGATACCCTTC CTGTCACAGATTCAAGCGGTGTGTGGGACGGGGATGTCCAGAACCCTGGAGGTGGCTTGATAAAGGGAGG TCTCAAAGGACAAAAGGGAGAGCCAGGTGCTCAAGGCCCACCTGGCCCAGCTGGGCCCCAGGGTCCTGCC GGTCCAGCGGTACAGAGCCCCAGCTCACAACCTGTCCCTGGAGCACAAGGACCCCCGGGACCTCAGGGAC CACCAGGGAAGGATGGCATCCCGGGAAGGGATGGTGAACCAGGCGACCCTGGTGAAGACGGGAGGCCGGG TGACACTGGACCTCAAGGCTTCCCGGGGACCCCAGGAGATGTGGGCCCTAAGGGTGAGAAGGGAGATCCT GGTATTGGGCCTCGAGGACCTCCAGGACCTCCGGGACCACCAGGACCCTCCTTCAGACAGGACAAGCTGA CCTTCATTGACATGGAGGGATCTGGTGGTTTCAGCGGAGACCTAGAGAGCCTCAGAGGCCCACGAGGCTT CCCTGGTCCCCCAGGTCCCCCTGGTGTCCCAGGACTGCCTGGTGAACCAGGGCGCTTTGGAGTCAACAGT TCCTACGCACCAGGACCTGCAGGCCTTCCTGGTGTGCCTGGGAAGGAAGGGCCCCCTGGTTTTCCTGGTC CCCCAGGACCTCCAGGCAAAGAGGGCCCACCAGGAGTGGCCGGCCAGAAAGGCAGTGTTGGCGATGCAGG CAGCCCAGGACCCAAGGGGAGCAAAGGAGACCTTGGGCCCATTGGTATGCCTGGGAAGTCTGGCTTGCCG GGACTCCCTGGGCCAGTTGGACCCCCAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCAGGATTTGCTG CTGGATTTGATGACATGGAAGGTTCTGGAACACCTCTCTGGTCAACAGCCCGAAGCTCCGATGGGCTGCA GGGGGATCCTGGAGTGACTGGGCCACCTGGGGCCAAGGGAGAAGTTGGAGCAGACGGAGTCCAGGGCATC CCTGGTCTCCCAGGAAGAGAGGGTGTAGCTGGGCCCCCGGGACCCAAAGGAGAGAAAGGGACCCAGGGAG AAAAGGGAAACCCAGGAAAAGATGGAGTAGGTCGGCCAGGCCTCCCTGGCCCTCCAGGCCCCCCAGGGCC TGTGATCTATGTGTCAAATGAGGATAGAGCTGTAGTAAGCACGCCGGGACCTGAGGGCAAGCCAGGGTAC GCAGGCTTTCCTGGACCTGCTGGACCGAAGGGTGACCTGGGTTCCAAAGGCGAGCAGGGTCTTCCAGGGC CCAAGGGTGAGAAGGGAGAACCAGGCAGTATCTTTAGCCCTGATGGCACAGCCCTGGGCCAGGCCCAGAA GGGAGCCAAGGGAGAACCAGGCTTCCGAGGACCCCCGGGTCCTTATGGACGGCCTGGGTACAAGGGTGAA ATTGGCTTCCCTGGACGGCCGGGTCGTCCCGGAACAAATGGCTTAAAGGGAGAGAAAGGGGAGCCTGGAG AGGCCAGCCTTGGGTTCAGCATGAGGGGACTGCCTGGCCCCCCTGGGCCTCCAGGCCCCCCAGGTCCTCC CGGAGTGCCCGTCTATGACAGCAATGCATTTGTGGAGTCTGGCCGACCTGGACTACCAGGACAGCAAGGT GTGCAGGGGCCTCCAGGACCAAAGGGTGACAAAGGAGAGGTGGGCCCACCCGGGCCACCAGGGCAGTTCC CCATTGACCTCTTCCACCTGGAAGCCGAAATGAAGGGGGACAAGGGAGACCGAGGGGATGCTGGACGGAA GGGAGAGAGGGGAGAGCCTGGGGCTCCTGGTGGTGGATTCTTCAGCTCAAGTGTACCTGGACCACCTGGC CCACCTGGATACCCTGGAATTCCGGGTCCAAAAGGAGAGAGCATTCGGGGTCCGCCTGGCCCTCCTGGCC CGCAGGGACCTCCTGGCATTGGATATGAGGGGCGCCAGGGCCCCCCAGGACCTCCAGGACCTCCAGGACC TCCCTCATTCCCTGGCCCTCATAGACAGACTGTCAGTGTTCCTGGTCCTCCAGGCCCACCTGGCCCCCCA GGTCCCCCAGGAGCCATGGGTGCCTCTGCTGGGCAGGTAAGGATCTGGGCTACATACCAGACCATGTTGG ACAAGATACGTGAGGTGCCAGAGGGCTGGCTCATCTTTGTGGCCGAGAGGGAAGAGCTCTACGTACGGGT TAGAAATGGCTTCCGTAAGGTGCTGCTGGAGGCCCGGACAGCCCTCCCGCACGGGACGGACAACGAGGTA GCTGCCTTGCAACCTCCATTGGTACAGCTTCATGAGGGCAGTTCATATACCCGGAGGGAGCACTCCTATC CCACGGCACGACCCTGGCGAGCAGATGACATCTTGGCCAACCCACCACGTCTGCCGGACCGCCAGCCTTA CCCGGGAGTTCCACACCACCACCACCACCACCACCACCACCACAGTTCTCATGAACACCGGCCGCCAGCC CACCCCTCCCCCTCACCTGCCCATACTCACCAGGACTTTCACCCAGTGCTCCACCTGGTGGCACTGAACA CCCCTCTGTCTGGAGGCATGCGTGGTATCCGTGGGGCTGACTTCCAGTGCTTCCAGCAAGCCCGAGCCGT GGGGCTGTCTGGCACCTTTCGGGCCTTCCTGTCCTCTAGGCTGCAGGACCTCTACAGCATTGTACGCCGT GCTGACCGCAGCTCTGTGCCCATCGTCAACCTGAAGGATGAGGTACTATCTCCCAGCTGGGACACCCTGT TCTCCGGCTCCCAGGGTCAACTGCACTCTGGGGCCCGCATCTTTTCTTTCGACGGCAGAGACGTCCTGAG ACACCCAGCCTGGCCACAGAAGAGCGTATGGCATGGCTCAGACCCCAGTGGGCGCAGGCTGATGGAGAGC TACTGTGAGACGTGGAGGACTGAAGCTACTGGGGTTACAGGTCAGGCCTCTTCCCTGCTGTCGGGCAGGC TCCTGGAACAGAAGGCCGAGAGCTGCCACAACAGCTATATCGTCCTGTGCATTGAGAATAGCTTCATGAC CTCTTTCTCCAAATAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_053489
Insert Size	3936 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053489.2, NP_445941.2
RefSeq Size	5007 bp
RefSeq ORF	3936 bp
Locus ID	85251
Gene Summary	intermediate filament protein; a precursor of the angiogenesis inhibitor endostatin [RGD, Feb 2006]

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