Col18a1 (NM_053489) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217584 representing NM_053489 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCAGGTGGCACCTCCTGGACATGCTCACCAGTTTGGTCTTGCTGCTGGTGGCACGCGTCTCCT GGGCAGAGCCAGAGAATGTTGCTGAAGATGTGGGGCTCCTGCAGCTCCTCGGAGACCCCCTACCCCAGAA GATCTCCCAAGTCGAAGACCCTCATGTCGGGCTGGCCTACGTCTTTGGACCATACTCCAAAAGTAGTCAG ATGGCCCAGTATCACTTCCCTAAACTCTTCTTCCGGGACTTTTCGCTGCTGTTCGAAGTCCGGCCAACCA CAGAGGCCGCAGGGGTGCTGTTTGCTATCACAGATGCCGCCCAGGTGGTGGTCTCGCTAGGCGTGAAGCT CTCAGAGGTCCGAGATGGACAACAGAACATCTCATTGCTCTACACGGAGCCCGGGGCCAGCCAGACCCAG ACGGGAGCCAGCTTCCGCCTACCTGCGTTTGTTGGCCAGTGGACACACTTCGCGCTCAGCGTGGATGGAA GCTCCGTGGCTCTCTACGTAGACTGTGAAGAGTTCCAGAGAGTGCCGTTCGCTCGGTCCCCACACGGTCT GGAGCTAGAGCGTGGCGCTGGCCTCTTTGTGGGTCAGGCTGGAGCAGCGGACCCTGACAAGTTCCAGGGA ATGATCTCAGAGCTGAGAGTACGCAAAACCCCCCGGGTGAGCCCTGTGCACTGCCTGGATGAAGAAGATG ATGATGACGACCGGGCATCTGGAGATTTTGGAAGTGGCCTCGAAGAAAGCAGCAATTTACACAGGCAGGA GACATATCTACGACCCGGGCTCCCCCAGCCACCTCCTGTCACTTCCCCGCCCTTGGCTGGAGGCAGTGCC ACCGAAGATTCTAGGACAGAAGAAAAGGAGGAAGAAGCCACGGTAGATTCTAAAGGAGCTGATACCCTTC CTGTCACAGATTCAAGCGGTGTGTGGGACGGGGATGTCCAGAACCCTGGAGGTGGCTTGATAAAGGGAGG TCTCAAAGGACAAAAGGGAGAGCCAGGTGCTCAAGGCCCACCTGGCCCAGCTGGGCCCCAGGGTCCTGCC GGTCCAGCGGTACAGAGCCCCAGCTCACAACCTGTCCCTGGAGCACAAGGACCCCCGGGACCTCAGGGAC CACCAGGGAAGGATGGCATCCCGGGAAGGGATGGTGAACCAGGCGACCCTGGTGAAGACGGGAGGCCGGG TGACACTGGACCTCAAGGCTTCCCGGGGACCCCAGGAGATGTGGGCCCTAAGGGTGAGAAGGGAGATCCT GGTATTGGGCCTCGAGGACCTCCAGGACCTCCGGGACCACCAGGACCCTCCTTCAGACAGGACAAGCTGA CCTTCATTGACATGGAGGGATCTGGTGGTTTCAGCGGAGACCTAGAGAGCCTCAGAGGCCCACGAGGCTT CCCTGGTCCCCCAGGTCCCCCTGGTGTCCCAGGACTGCCTGGTGAACCAGGGCGCTTTGGAGTCAACAGT TCCTACGCACCAGGACCTGCAGGCCTTCCTGGTGTGCCTGGGAAGGAAGGGCCCCCTGGTTTTCCTGGTC CCCCAGGACCTCCAGGCAAAGAGGGCCCACCAGGAGTGGCCGGCCAGAAAGGCAGTGTTGGCGATGCAGG CAGCCCAGGACCCAAGGGGAGCAAAGGAGACCTTGGGCCCATTGGTATGCCTGGGAAGTCTGGCTTGCCG GGACTCCCTGGGCCAGTTGGACCCCCAGGACCTCCAGGGCCTCCAGGGCCACCAGGACCAGGATTTGCTG CTGGATTTGATGACATGGAAGGTTCTGGAACACCTCTCTGGTCAACAGCCCGAAGCTCCGATGGGCTGCA GGGGGATCCTGGAGTGACTGGGCCACCTGGGGCCAAGGGAGAAGTTGGAGCAGACGGAGTCCAGGGCATC CCTGGTCTCCCAGGAAGAGAGGGTGTAGCTGGGCCCCCGGGACCCAAAGGAGAGAAAGGGACCCAGGGAG AAAAGGGAAACCCAGGAAAAGATGGAGTAGGTCGGCCAGGCCTCCCTGGCCCTCCAGGCCCCCCAGGGCC TGTGATCTATGTGTCAAATGAGGATAGAGCTGTAGTAAGCACGCCGGGACCTGAGGGCAAGCCAGGGTAC GCAGGCTTTCCTGGACCTGCTGGACCGAAGGGTGACCTGGGTTCCAAAGGCGAGCAGGGTCTTCCAGGGC CCAAGGGTGAGAAGGGAGAACCAGGCAGTATCTTTAGCCCTGATGGCACAGCCCTGGGCCAGGCCCAGAA GGGAGCCAAGGGAGAACCAGGCTTCCGAGGACCCCCGGGTCCTTATGGACGGCCTGGGTACAAGGGTGAA ATTGGCTTCCCTGGACGGCCGGGTCGTCCCGGAACAAATGGCTTAAAGGGAGAGAAAGGGGAGCCTGGAG AGGCCAGCCTTGGGTTCAGCATGAGGGGACTGCCTGGCCCCCCTGGGCCTCCAGGCCCCCCAGGTCCTCC CGGAGTGCCCGTCTATGACAGCAATGCATTTGTGGAGTCTGGCCGACCTGGACTACCAGGACAGCAAGGT GTGCAGGGGCCTCCAGGACCAAAGGGTGACAAAGGAGAGGTGGGCCCACCCGGGCCACCAGGGCAGTTCC CCATTGACCTCTTCCACCTGGAAGCCGAAATGAAGGGGGACAAGGGAGACCGAGGGGATGCTGGACGGAA GGGAGAGAGGGGAGAGCCTGGGGCTCCTGGTGGTGGATTCTTCAGCTCAAGTGTACCTGGACCACCTGGC CCACCTGGATACCCTGGAATTCCGGGTCCAAAAGGAGAGAGCATTCGGGGTCCGCCTGGCCCTCCTGGCC CGCAGGGACCTCCTGGCATTGGATATGAGGGGCGCCAGGGCCCCCCAGGACCTCCAGGACCTCCAGGACC TCCCTCATTCCCTGGCCCTCATAGACAGACTGTCAGTGTTCCTGGTCCTCCAGGCCCACCTGGCCCCCCA GGTCCCCCAGGAGCCATGGGTGCCTCTGCTGGGCAGGTAAGGATCTGGGCTACATACCAGACCATGTTGG ACAAGATACGTGAGGTGCCAGAGGGCTGGCTCATCTTTGTGGCCGAGAGGGAAGAGCTCTACGTACGGGT TAGAAATGGCTTCCGTAAGGTGCTGCTGGAGGCCCGGACAGCCCTCCCGCACGGGACGGACAACGAGGTA GCTGCCTTGCAACCTCCATTGGTACAGCTTCATGAGGGCAGTTCATATACCCGGAGGGAGCACTCCTATC CCACGGCACGACCCTGGCGAGCAGATGACATCTTGGCCAACCCACCACGTCTGCCGGACCGCCAGCCTTA CCCGGGAGTTCCACACCACCACCACCACCACCACCACCACCACAGTTCTCATGAACACCGGCCGCCAGCC CACCCCTCCCCCTCACCTGCCCATACTCACCAGGACTTTCACCCAGTGCTCCACCTGGTGGCACTGAACA CCCCTCTGTCTGGAGGCATGCGTGGTATCCGTGGGGCTGACTTCCAGTGCTTCCAGCAAGCCCGAGCCGT GGGGCTGTCTGGCACCTTTCGGGCCTTCCTGTCCTCTAGGCTGCAGGACCTCTACAGCATTGTACGCCGT GCTGACCGCAGCTCTGTGCCCATCGTCAACCTGAAGGATGAGGTACTATCTCCCAGCTGGGACACCCTGT TCTCCGGCTCCCAGGGTCAACTGCACTCTGGGGCCCGCATCTTTTCTTTCGACGGCAGAGACGTCCTGAG ACACCCAGCCTGGCCACAGAAGAGCGTATGGCATGGCTCAGACCCCAGTGGGCGCAGGCTGATGGAGAGC TACTGTGAGACGTGGAGGACTGAAGCTACTGGGGTTACAGGTCAGGCCTCTTCCCTGCTGTCGGGCAGGC TCCTGGAACAGAAGGCCGAGAGCTGCCACAACAGCTATATCGTCCTGTGCATTGAGAATAGCTTCATGAC CTCTTTCTCCAAA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217584 representing NM_053489 Red=Cloning site Green=Tags(s) MAPRWHLLDMLTSLVLLLVARVSWAEPENVAEDVGLLQLLGDPLPQKISQVEDPHVGLAYVFGPYSKSSQ MAQYHFPKLFFRDFSLLFEVRPTTEAAGVLFAITDAAQVVVSLGVKLSEVRDGQQNISLLYTEPGASQTQ TGASFRLPAFVGQWTHFALSVDGSSVALYVDCEEFQRVPFARSPHGLELERGAGLFVGQAGAADPDKFQG MISELRVRKTPRVSPVHCLDEEDDDDDRASGDFGSGLEESSNLHRQETYLRPGLPQPPPVTSPPLAGGSA TEDSRTEEKEEEATVDSKGADTLPVTDSSGVWDGDVQNPGGGLIKGGLKGQKGEPGAQGPPGPAGPQGPA GPAVQSPSSQPVPGAQGPPGPQGPPGKDGIPGRDGEPGDPGEDGRPGDTGPQGFPGTPGDVGPKGEKGDP GIGPRGPPGPPGPPGPSFRQDKLTFIDMEGSGGFSGDLESLRGPRGFPGPPGPPGVPGLPGEPGRFGVNS SYAPGPAGLPGVPGKEGPPGFPGPPGPPGKEGPPGVAGQKGSVGDAGSPGPKGSKGDLGPIGMPGKSGLP GLPGPVGPPGPPGPPGPPGPGFAAGFDDMEGSGTPLWSTARSSDGLQGDPGVTGPPGAKGEVGADGVQGI PGLPGREGVAGPPGPKGEKGTQGEKGNPGKDGVGRPGLPGPPGPPGPVIYVSNEDRAVVSTPGPEGKPGY AGFPGPAGPKGDLGSKGEQGLPGPKGEKGEPGSIFSPDGTALGQAQKGAKGEPGFRGPPGPYGRPGYKGE IGFPGRPGRPGTNGLKGEKGEPGEASLGFSMRGLPGPPGPPGPPGPPGVPVYDSNAFVESGRPGLPGQQG VQGPPGPKGDKGEVGPPGPPGQFPIDLFHLEAEMKGDKGDRGDAGRKGERGEPGAPGGGFFSSSVPGPPG PPGYPGIPGPKGESIRGPPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTVSVPGPPGPPGPP GPPGAMGASAGQVRIWATYQTMLDKIREVPEGWLIFVAEREELYVRVRNGFRKVLLEARTALPHGTDNEV AALQPPLVQLHEGSSYTRREHSYPTARPWRADDILANPPRLPDRQPYPGVPHHHHHHHHHHSSHEHRPPA HPSPSPAHTHQDFHPVLHLVALNTPLSGGMRGIRGADFQCFQQARAVGLSGTFRAFLSSRLQDLYSIVRR ADRSSVPIVNLKDEVLSPSWDTLFSGSQGQLHSGARIFSFDGRDVLRHPAWPQKSVWHGSDPSGRRLMES YCETWRTEATGVTGQASSLLSGRLLEQKAESCHNSYIVLCIENSFMTSFSK TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053489
ORF Size	3933 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053489.2, NP_445941.2
RefSeq Size	5007 bp
RefSeq ORF	3936 bp
Locus ID	85251
MW	134.6 kDa
Gene Summary	intermediate filament protein; a precursor of the angiogenesis inhibitor endostatin [RGD, Feb 2006]

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