Synpo2 (NM_001191963) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1564779
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217568 representing NM_001191963 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGGAGATTTCATCTGCATTTCCATGACTGGAGGGGCGCCCTGGGGGTTCAGGCTGCAAGGAG GCAAGGAGGAGAAGCAGCCCTTGCAAGTGGCAAAGATTCGAAGTCAGAGCAAGGCATCGGACTCCGGGCT GTGTGTTGGGGATGAAGTGGTGTCCATCAATGGTAACCCTTGTGCAGACCTTACTTACCCTGAAGTCATC AAACTCATGGAAAGCATAACGGACTCCCTCCATCTGCTTATTAAAAGACCCACCAGTGGAACAAGTGAGG CTTTGGATTCTGAAACAGAAAATACAAACCACCAGCACCTTCCACACGGGGGGCCCATGGAAAGTACCAC CCTGCAGATCCAGCACGCTGCCAAGACCCAGGGCAAAGACTTCCTTCTTGCTTCAGTCCAGACTAGTGCT CCCCGAACTGAGGACCAAGGAAATGCCTGGGGTTATGCAGAGTGTACAACAGAAGACCAAGTTTCCCAGA TGCCTGGCTCCCAAGAAGGACACTTGGTAGAAGAGGTCATCTTAAGGAAAAAGCCAGAAGCAGGCCAGCC AGGCCATGTGGTTGAGTTACAGCTGTCCCTCTCAAAGGAAAGACAGCGATGCACCAGTGACCCTATAGTG ACTCTCCTGGGAAATGAAAAATTCAAGTCTCCAGACCCAGACTGGGGCACCCAACACGGGAGGACTGTCC ACATAAATTCAATCCCTGCTCCTGAGAAAGCAGACACTTCTCTGACGTCCGGCACCACAGTCCAGACCTC CAGTGGCCGGGAGCTGACAGTGATCCAGGGAAGAGACCCAGGAGGCACAGGGCTGCCCCAGGTGGAAGTG ATTCTAGATTGCTCTGACAGGCTGAAGGCTGAAGAGTGCAGGCTGCAGGCAGGAAGGGGGTGTGTGGCTT CTCCCGTGGAAGGAGGGCGGTCAGAAGCACCTCCTTCTCTGGTCTCCTTTGCGGTCTCATCAGAAGGCAC CGAGCAGGGAGAAGATCAACGCTCGGGAAAGGACCAGGGCAGACCACACAAGCACAGAGCGAGGCATGCC CGGCTCAGGAGGAGTGAAAGTCTGTCTGAAAAGCAAGTAAAAGAAGCCAAGTCTAAATGCAAAAGCATCG CCCTCCTCCTAACGGATGCTCCCAACCCGAACTCCAAGGGGGTGTTGATGTTTAAGAAGCGGAGGCGGAG AGCCCGGAAGTACACCCTAGTCAGTTACGGTACTGGAGAGCTTGAGCGAGAGGAGGAAGAGGAGGACCAG GAAGCAGGTGACAAGGACGAGATAAGTGACCTTGCATTTCTTGGAACCAGCGAGTCGGAAGTGGATGAAG AGTTACTGTCTGACGTGGACGACAACACCCAAGTTGTGAACTTCGATTGGGATTCTGGACTGGTGGACAT TGAAAAGAGGCTGAACCGAGGCGACAAGATGGAGATGTTGCCCGACACCACAGGCAAGGGAGCGCTCATG TTTGCCAAGAGGAGAGAGAGGATGGAACAGTTCACAGCCCAAAATGAAGAGGAAAAGACCGGTGGGCTAG CAGGCGGAGGATCAGATGCCCTACAGACGGACGGTCTGAGAACCATGACTTCTTATCAAAGAAAAGAAGA ATCGGTGAGAATGCAGAGCTCTGTGAGTGAGAGCTCCTTCCAGATGGGACGCAGTCTTGGCAGTGTGCCA CAACAGAATGGCTTCAGTGGAGTGTCTGAGACAGCAGGGCCCCAGAGGATGATCCCCATGAACAGAACCG CCAAGCCCTTCCTGGGATCTGTGAACCAGACAGCGGCCCCATTCTCCCCAACACAGAGTGTGACAAGTCC CATCCCTGACTTCCCTGCGCCACCACCATACTCTGCAGTCTCACCTCCACCAGAAGCCTTCTCCAGGGGG ATATCAAGCCCAGTTGCTGGCCCGGCACAGCCCCCTCCGTGGCCCCAGCCTGCTCCATGGTCGCAACCAG CCTTCTATGATTCTTCTGAGCAAATAGCTTCCCGCGATGAGAGGATCGCAGTGCCGGCCAAAAGAACAGG AATACTGCAGGAAGCCAAAAGACGAGGCACAACAAAACCCATGTTTACTTTCAAAGAAACCAAAGTGAGC CCCAACCCCGAACTCCTGTCCCTTCTTCAAAATGCAGAAGGCAAGCGAGGCACTGGAGCTGGAGGGGACT CCGGGCCAGAAGAAGACTACCTCAGTTTAGGCGCAGAGGCTTGTAATTTCATGCAGAGTTCTGCCAAACA AAAGACCCCTCCTCCCGTCGCTCCAAAGCCTGCAGTCAAGACCTCCTCTTCCTCCCAACCAGTAGCCCCT GTTTCTCCAGTCTGGTCTCCAGGAGTGGCTCCAGCCCAGGGTCCTGCCTTCTCTACAACAAACCCACCAA ACCCACCACAGGTCACTGCTGTGTCCTCCATCAAAATAGCCCAGCCTACCTGCCCTCCAGCCCGGCCCGC GAGTGCACTGAACCTGGCTGGTCCTTTCAAAGGTCCCCAAGCAGCAGTAGTCAGTCATAACTATACACCC AAGCCATCAGCACCCACACCATTAGTAAATGCTGCTCCTGCTGGTGCCGGGGGACCATCCAATGAGCTTC CTGGAATGAGTGGGAAAGGAGCCCAACTCTTCGCTAAAAGGCAGTCTAGGATGGAGAAGTATGTGGTAGA TTCTGACACGGTGCAGGCCCACACAGTTCGGGCCCAGTCTCCTACTCCATCCCTGCCCGCCAGTTGGAAG TACTCCTCCAATGTCCGAGCACCGCCTCCGGTGGCCTACAATCCTATCCATTCCCCGTCTTACCCACTGG CTGCTATCAAGTCTCAGCCACCGGGAGCCCAGGCCTCCAAAACGAGCAAGAAAAAGGGCAAGAAACCTCT TAACACTTTGGATGTCATGAAGCACCAACCATATCAGCTCAACGCGTCTTTGTTTACTTTTCAACCCCCC GATTCAAAGGATGGCCTCCCTCAAAAGTCAACAGTCAAGGTCAGTTCAGTAGCCCCTGCCATGAAGCAAG CCCTTCCTCCCCGGCAGGCGGATATTGGCTCTCCCACAAACGCGAAGGCTTCATCTGTGTATTCAGTCCC GGCCTATACCTCTCAGCCCAACTTCTTTGCGGAGGCCACCTCACCAGTCAGCGCATCCCCGGTGCCTGTG AGTGTTCCCACCTCTCCAAAGCAAGAAACGACCTCCACATCCTATTTTGTGGCTCCGAGGCCGAAGTTCT CAGCCAAGAAAAGTGGTGTCACAGTTCAGGAGAACTGGCGCTCCCTTTCTCTCCCCGGAAGAGCAGCTCC GCCCATCATGTCTGCACCTCCCTGGCTGTGCCAGCCTGCCTACAGTTACTCCAGCAAACCAACCCTCGAG CAAGAGAAAGCTAACAAGAGACCTACACCTTGGGAGGCGGCGGCCAAGTCCCCTCTCGGTCTAGTGGATG AGGCCTTCAGACCCAGGAACATCGAGGAATCCATTGTGGCGAATGTAGTCTCTGCAGCTCGGAGGAAGGT GTTTGCAGGGTCCCAAGAGGACTGGAAAGAGAGACTGTCCTTTGTCCCTCAAACTCAGAAGACCAGCATG AGCTTTTCTGAAAGGCGAGAGTATAATGTTCCATCCCCAGTCAACAGCCACGTGTCTAGCCACTCCTTAT ATAGCTCCCAGTTGCCATATGTATGCTACAGGAAGGAGTCCAGAAATGATTTGAAAGCAATGTCCATGGA CACGAGGTCTGAATACTGTCTTCCACTAGGTGGTTATGACTATAACCCACACCCAAGGGGGTGGAGACAC CAACCATGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001191963
Insert Size	3789 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191963.1, NP_001178892.1
RefSeq Size	4092 bp
RefSeq ORF	3789 bp
Locus ID	499702
UniProt ID	D4A702
Gene Summary	Has an actin-binding and actin-bundling activity. Can induce the formation of F-actin networks. At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines (By similarity). Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension; may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes (PubMed:23434281). Involved in regulation of cell migration. May be a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]

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