Synpo2 (NM_001191963) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1564779
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217568 representing NM_001191963 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACCGGAGATTTCATCTGCATTTCCATGACTGGAGGGGCGCCCTGGGGGTTCAGGCTGCAAGGAG GCAAGGAGGAGAAGCAGCCCTTGCAAGTGGCAAAGATTCGAAGTCAGAGCAAGGCATCGGACTCCGGGCT GTGTGTTGGGGATGAAGTGGTGTCCATCAATGGTAACCCTTGTGCAGACCTTACTTACCCTGAAGTCATC AAACTCATGGAAAGCATAACGGACTCCCTCCATCTGCTTATTAAAAGACCCACCAGTGGAACAAGTGAGG CTTTGGATTCTGAAACAGAAAATACAAACCACCAGCACCTTCCACACGGGGGGCCCATGGAAAGTACCAC CCTGCAGATCCAGCACGCTGCCAAGACCCAGGGCAAAGACTTCCTTCTTGCTTCAGTCCAGACTAGTGCT CCCCGAACTGAGGACCAAGGAAATGCCTGGGGTTATGCAGAGTGTACAACAGAAGACCAAGTTTCCCAGA TGCCTGGCTCCCAAGAAGGACACTTGGTAGAAGAGGTCATCTTAAGGAAAAAGCCAGAAGCAGGCCAGCC AGGCCATGTGGTTGAGTTACAGCTGTCCCTCTCAAAGGAAAGACAGCGATGCACCAGTGACCCTATAGTG ACTCTCCTGGGAAATGAAAAATTCAAGTCTCCAGACCCAGACTGGGGCACCCAACACGGGAGGACTGTCC ACATAAATTCAATCCCTGCTCCTGAGAAAGCAGACACTTCTCTGACGTCCGGCACCACAGTCCAGACCTC CAGTGGCCGGGAGCTGACAGTGATCCAGGGAAGAGACCCAGGAGGCACAGGGCTGCCCCAGGTGGAAGTG ATTCTAGATTGCTCTGACAGGCTGAAGGCTGAAGAGTGCAGGCTGCAGGCAGGAAGGGGGTGTGTGGCTT CTCCCGTGGAAGGAGGGCGGTCAGAAGCACCTCCTTCTCTGGTCTCCTTTGCGGTCTCATCAGAAGGCAC CGAGCAGGGAGAAGATCAACGCTCGGGAAAGGACCAGGGCAGACCACACAAGCACAGAGCGAGGCATGCC CGGCTCAGGAGGAGTGAAAGTCTGTCTGAAAAGCAAGTAAAAGAAGCCAAGTCTAAATGCAAAAGCATCG CCCTCCTCCTAACGGATGCTCCCAACCCGAACTCCAAGGGGGTGTTGATGTTTAAGAAGCGGAGGCGGAG AGCCCGGAAGTACACCCTAGTCAGTTACGGTACTGGAGAGCTTGAGCGAGAGGAGGAAGAGGAGGACCAG GAAGCAGGTGACAAGGACGAGATAAGTGACCTTGCATTTCTTGGAACCAGCGAGTCGGAAGTGGATGAAG AGTTACTGTCTGACGTGGACGACAACACCCAAGTTGTGAACTTCGATTGGGATTCTGGACTGGTGGACAT TGAAAAGAGGCTGAACCGAGGCGACAAGATGGAGATGTTGCCCGACACCACAGGCAAGGGAGCGCTCATG TTTGCCAAGAGGAGAGAGAGGATGGAACAGTTCACAGCCCAAAATGAAGAGGAAAAGACCGGTGGGCTAG CAGGCGGAGGATCAGATGCCCTACAGACGGACGGTCTGAGAACCATGACTTCTTATCAAAGAAAAGAAGA ATCGGTGAGAATGCAGAGCTCTGTGAGTGAGAGCTCCTTCCAGATGGGACGCAGTCTTGGCAGTGTGCCA CAACAGAATGGCTTCAGTGGAGTGTCTGAGACAGCAGGGCCCCAGAGGATGATCCCCATGAACAGAACCG CCAAGCCCTTCCTGGGATCTGTGAACCAGACAGCGGCCCCATTCTCCCCAACACAGAGTGTGACAAGTCC CATCCCTGACTTCCCTGCGCCACCACCATACTCTGCAGTCTCACCTCCACCAGAAGCCTTCTCCAGGGGG ATATCAAGCCCAGTTGCTGGCCCGGCACAGCCCCCTCCGTGGCCCCAGCCTGCTCCATGGTCGCAACCAG CCTTCTATGATTCTTCTGAGCAAATAGCTTCCCGCGATGAGAGGATCGCAGTGCCGGCCAAAAGAACAGG AATACTGCAGGAAGCCAAAAGACGAGGCACAACAAAACCCATGTTTACTTTCAAAGAAACCAAAGTGAGC CCCAACCCCGAACTCCTGTCCCTTCTTCAAAATGCAGAAGGCAAGCGAGGCACTGGAGCTGGAGGGGACT CCGGGCCAGAAGAAGACTACCTCAGTTTAGGCGCAGAGGCTTGTAATTTCATGCAGAGTTCTGCCAAACA AAAGACCCCTCCTCCCGTCGCTCCAAAGCCTGCAGTCAAGACCTCCTCTTCCTCCCAACCAGTAGCCCCT GTTTCTCCAGTCTGGTCTCCAGGAGTGGCTCCAGCCCAGGGTCCTGCCTTCTCTACAACAAACCCACCAA ACCCACCACAGGTCACTGCTGTGTCCTCCATCAAAATAGCCCAGCCTACCTGCCCTCCAGCCCGGCCCGC GAGTGCACTGAACCTGGCTGGTCCTTTCAAAGGTCCCCAAGCAGCAGTAGTCAGTCATAACTATACACCC AAGCCATCAGCACCCACACCATTAGTAAATGCTGCTCCTGCTGGTGCCGGGGGACCATCCAATGAGCTTC CTGGAATGAGTGGGAAAGGAGCCCAACTCTTCGCTAAAAGGCAGTCTAGGATGGAGAAGTATGTGGTAGA TTCTGACACGGTGCAGGCCCACACAGTTCGGGCCCAGTCTCCTACTCCATCCCTGCCCGCCAGTTGGAAG TACTCCTCCAATGTCCGAGCACCGCCTCCGGTGGCCTACAATCCTATCCATTCCCCGTCTTACCCACTGG CTGCTATCAAGTCTCAGCCACCGGGAGCCCAGGCCTCCAAAACGAGCAAGAAAAAGGGCAAGAAACCTCT TAACACTTTGGATGTCATGAAGCACCAACCATATCAGCTCAACGCGTCTTTGTTTACTTTTCAACCCCCC GATTCAAAGGATGGCCTCCCTCAAAAGTCAACAGTCAAGGTCAGTTCAGTAGCCCCTGCCATGAAGCAAG CCCTTCCTCCCCGGCAGGCGGATATTGGCTCTCCCACAAACGCGAAGGCTTCATCTGTGTATTCAGTCCC GGCCTATACCTCTCAGCCCAACTTCTTTGCGGAGGCCACCTCACCAGTCAGCGCATCCCCGGTGCCTGTG AGTGTTCCCACCTCTCCAAAGCAAGAAACGACCTCCACATCCTATTTTGTGGCTCCGAGGCCGAAGTTCT CAGCCAAGAAAAGTGGTGTCACAGTTCAGGAGAACTGGCGCTCCCTTTCTCTCCCCGGAAGAGCAGCTCC GCCCATCATGTCTGCACCTCCCTGGCTGTGCCAGCCTGCCTACAGTTACTCCAGCAAACCAACCCTCGAG CAAGAGAAAGCTAACAAGAGACCTACACCTTGGGAGGCGGCGGCCAAGTCCCCTCTCGGTCTAGTGGATG AGGCCTTCAGACCCAGGAACATCGAGGAATCCATTGTGGCGAATGTAGTCTCTGCAGCTCGGAGGAAGGT GTTTGCAGGGTCCCAAGAGGACTGGAAAGAGAGACTGTCCTTTGTCCCTCAAACTCAGAAGACCAGCATG AGCTTTTCTGAAAGGCGAGAGTATAATGTTCCATCCCCAGTCAACAGCCACGTGTCTAGCCACTCCTTAT ATAGCTCCCAGTTGCCATATGTATGCTACAGGAAGGAGTCCAGAAATGATTTGAAAGCAATGTCCATGGA CACGAGGTCTGAATACTGTCTTCCACTAGGTGGTTATGACTATAACCCACACCCAAGGGGGTGGAGACAC CAACCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217568 representing NM_001191963 Red=Cloning site Green=Tags(s) MGTGDFICISMTGGAPWGFRLQGGKEEKQPLQVAKIRSQSKASDSGLCVGDEVVSINGNPCADLTYPEVI KLMESITDSLHLLIKRPTSGTSEALDSETENTNHQHLPHGGPMESTTLQIQHAAKTQGKDFLLASVQTSA PRTEDQGNAWGYAECTTEDQVSQMPGSQEGHLVEEVILRKKPEAGQPGHVVELQLSLSKERQRCTSDPIV TLLGNEKFKSPDPDWGTQHGRTVHINSIPAPEKADTSLTSGTTVQTSSGRELTVIQGRDPGGTGLPQVEV ILDCSDRLKAEECRLQAGRGCVASPVEGGRSEAPPSLVSFAVSSEGTEQGEDQRSGKDQGRPHKHRARHA RLRRSESLSEKQVKEAKSKCKSIALLLTDAPNPNSKGVLMFKKRRRRARKYTLVSYGTGELEREEEEEDQ EAGDKDEISDLAFLGTSESEVDEELLSDVDDNTQVVNFDWDSGLVDIEKRLNRGDKMEMLPDTTGKGALM FAKRRERMEQFTAQNEEEKTGGLAGGGSDALQTDGLRTMTSYQRKEESVRMQSSVSESSFQMGRSLGSVP QQNGFSGVSETAGPQRMIPMNRTAKPFLGSVNQTAAPFSPTQSVTSPIPDFPAPPPYSAVSPPPEAFSRG ISSPVAGPAQPPPWPQPAPWSQPAFYDSSEQIASRDERIAVPAKRTGILQEAKRRGTTKPMFTFKETKVS PNPELLSLLQNAEGKRGTGAGGDSGPEEDYLSLGAEACNFMQSSAKQKTPPPVAPKPAVKTSSSSQPVAP VSPVWSPGVAPAQGPAFSTTNPPNPPQVTAVSSIKIAQPTCPPARPASALNLAGPFKGPQAAVVSHNYTP KPSAPTPLVNAAPAGAGGPSNELPGMSGKGAQLFAKRQSRMEKYVVDSDTVQAHTVRAQSPTPSLPASWK YSSNVRAPPPVAYNPIHSPSYPLAAIKSQPPGAQASKTSKKKGKKPLNTLDVMKHQPYQLNASLFTFQPP DSKDGLPQKSTVKVSSVAPAMKQALPPRQADIGSPTNAKASSVYSVPAYTSQPNFFAEATSPVSASPVPV SVPTSPKQETTSTSYFVAPRPKFSAKKSGVTVQENWRSLSLPGRAAPPIMSAPPWLCQPAYSYSSKPTLE QEKANKRPTPWEAAAKSPLGLVDEAFRPRNIEESIVANVVSAARRKVFAGSQEDWKERLSFVPQTQKTSM SFSERREYNVPSPVNSHVSSHSLYSSQLPYVCYRKESRNDLKAMSMDTRSEYCLPLGGYDYNPHPRGWRH QP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191963
ORF Size	3786 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191963.1, NP_001178892.1
RefSeq Size	4092 bp
RefSeq ORF	3789 bp
Locus ID	499702
UniProt ID	D4A702
MW	136 kDa
Gene Summary	Has an actin-binding and actin-bundling activity. Can induce the formation of F-actin networks. At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines (By similarity). Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension; may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes (PubMed:23434281). Involved in regulation of cell migration. May be a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]

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