Tyk2 (NM_001257347) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217534 representing NM_001257347 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACAAGCATGCCTCTGTGTGGGCGGAGAGCCGCCTCGGAAGACAGCAAGGCAGATGGCACAGAAG CTCAGCCCCTGGTGCCCACAGGACGGTTGATGGTGCTGCTGCACTGGCCCGGGCCTGAGGGCGGGGAGCC CTGGGTCACCTTCAGCCAGCCATCTCTGACTGCAGAGGAGATGTGCATCCACATCGCACACAAAGTCGGC ATCACTCCACCCTGCCTGAATCTCTTCGCACTCTACGATGCACAGGCCAAGGTCTGGCTGCCCCCAAACC ACGTTCTGGACACATCCCAAGACGCAACCCTGAGGCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTG GCATGGCATGAATCCCCAAGAGCCTGCTGTATACCGATGTGGTTTCCCAGGGGCAGAGACTTCCTCAGAC CATGCAGAGCAAGGTGTACAGCTCTTGGACTCTGCCTCATTTGAATACCTTTTTGAGCAGGGAAAACATG AATTCATGAACGATGTGGTATCTCTGCGGGACCTGTCTAGCGAGGAAGAGATCCACCACTTTAAGAATGA GAGCCTGGGCATGGCCTTTCTGCACCTCTGCCATCTTGCTCTCAACCGTGGCGTCCCCCTGGAGGAGATG GCCAGAGAGATCAGCTTCAAGAACTGTATCCCTCACTCCTTCCGTCAGCACATCCGCCAGCACAATGTGC TTACGCGCCTGCGTCTCCGCAGAGTCTTCCGCCGCTTCCTGCAGGCCTTCCAGCCTGGCCACCTCTCCCA ACAGGTTGTGATGGTTAAGTACTTGGCCACCCTGGAGCGGCTGGCTCCTCGTTTTGGCTCAGAGCACATA CCTGTGTGTCATCTGGAAGTTCTGGCCCAGCCTGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGA CCACTGGGGACCCAGGCCCAGAGCTGGCTTCTGGGCCAGCCACCCATGAGGTACTGGTGACAGGCACCGG AGGTATCCAGTGGCGTCCACTACAGACCCAGGACTCTGAGAGAGGTCACAGCAGAGGGAATCCCCAAGGC AGCCAGTCTGGGAAGAAACCCAAGGCCCCTGAGTCTGGAGAGCACCTGGCAGGGAGTCCCCAGGAACCAC CTTGGACCTACTTCTGTGACTTCCAGGACATTTCCCATGTGGTACTAAAGGAGCGTCGAGTGCACATCCA CCTTCAAGACAACAAGTGCTTGTTGCTGTGCCTCTGCTCCCAGGCCGAGGCCCTGTCCTTTGTGGCCCTG GTCGATGGCTATTTCCGCTTGACTGCTGACTCCAGCCACTACCTGTGCCATGAGGTAGCACCCCCGAGGC TGGTGACTAGCATCCAGAATGGCATCCACGGGCCCCTGATGGACCCATTTGTACAGGCTAAGCTGTGGCC AGAGGATGGCCTCTACCTGATCCAGTGGAGCACCAGCCACCTGCACCGACTGATCCTCACCGTAGCCCAT CGAAACCCGGCTTCCAGAAACGGCCCTAGGGGCCTACGCCTCCGAAAGTTCCCCATCACCCAGCAGCCTG GAGCCTTTGTGCTTGATGGTTGGGGCCGCTCCTTTGACAGCTTGGGGGACCTTCGGCAGGCCTTGCAGGG CTGCTCATTGCGAGCTGGTGATGACTGTTTCCCGTTGCACCACTGCTGCCTGCCCCGACCAAGAGAAATC TCCAACCTTGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCACTCAACCTCAGTCAGCTTAGCTTCC ACAGGGTTCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGA GGGCCTGCTAAGTGTGGGAGGCCCCAATGAGGGCAAAGTGGACAGTGGATGCCCCTCTGGGCCTGATAGG GGCAGTGGGCAGCAGCTTCGAGTGGTGCTCAAAGTCCTCGACCCTAGTCACCATGATATCGCCTTGGCCT TCTATGAGACTGCCAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTCTGCGT GCGTGGCTCAGAGAATATCATTGTAGCGGAATTCGTAGAGCACGGTCCCCTGGATGTGTGGTTACGGAGA CAGAGAGGCCGAGTGCCCATGCCCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTGAGCTACC TGGAGGACAAGAATCTGGTTCACGGGAACGTATGTGGCCGGAACATCCTGCTGGCCCGCCTGGGGCTGGA GGAGGGTACCAACCCCTTCATCAAGCTCAGTGACCCTGGCGTGGGCCAAGGCGCCCTCTCCAGGGAAGAG CGGGTGGAGCGAATCCCCTGGACAGCTCCGGAGTGCCTGTCCGGAGGGGCCAACAGCTTGGGCACTGCCA CGGACATGTGGGGCTTTGGTGCCACCCTCCTTGAGATCTGTTTTGATGGAGAGGCACCTCTGCAGGGCCG TGGTCCCTCCGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCAGAGCCCTCATGCCCGGAGCTG GCCACACTCACCCGTCAGTGCCTGAGCTATGAACCCGCACAGAGGCCATCGTTCCGCACCATTTTGCGGG ACCTCACAAGACTGCAGCCACAGAATCTAGTGGGAACTTCGACTGTGAACTCGGACTCACCAGCATCGGA CCCCACCGTTTTTCACAAGCGTTACTTGAAAAGGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTC AGCCTGTACTGCTATGACCCGAACAATGACGGCACTGGCGAGATGGTGGCTGTGAAAGCCCTGAAGGAGG GATGTGGTCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAAATCCTTCGGACGCTGTACCACGAGCA CATTGTCAAGTACAAAGGCTGCTGTGAAGACCAAGGGGAGAAGTCTGTACAGCTGGTCATGGAATACGTC CCTCTGGGCAGCCTCCGAGACTACCTGCCAAGGCACAGCGTGGGGCTGGCTCAGCTCCTGTTGTTTGCCC AGCAGATCTGCGAGGGCATGGCTTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCTGCGCGAAA CGTGCTGCTGGACAACGACCGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGC CACGAGTACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTATTCTGGTATGCCCCAGAATGCCTAAAGG AGTGCAAGTTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTGACCCTTTATGAGCTGTTGACATACTG TGACTCTAGCCAGAGTCCCCCCACGAAATTCATCGAGCTCATCGGCGTAACCCAGGGCCAGATGACTGTG CTGAGGCTCACAGAGCTGCTGGAGCGAGGGGAGAGGCTGCCGCGGCCTGACAGATGTCCCTGTGAGATCT ATCACCTCATGAAGAACTGCTGGGAGTCAGAGGCCTCCTTCCGGCCCACGTTCCAGAATCTTGTGCCCAT CCTTAAGACAGCACAGGAGAAGTACCAAGGCCAGGTGCCTTCAGTGTTCAGCGTGTGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257347
Insert Size	3561 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257347.1, NP_001244276.1
RefSeq Size	4535 bp
RefSeq ORF	3561 bp
Locus ID	100361294

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