Tyk2 (NM_001257347) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217534 representing NM_001257347 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGACAAGCATGCCTCTGTGTGGGCGGAGAGCCGCCTCGGAAGACAGCAAGGCAGATGGCACAGAAG CTCAGCCCCTGGTGCCCACAGGACGGTTGATGGTGCTGCTGCACTGGCCCGGGCCTGAGGGCGGGGAGCC CTGGGTCACCTTCAGCCAGCCATCTCTGACTGCAGAGGAGATGTGCATCCACATCGCACACAAAGTCGGC ATCACTCCACCCTGCCTGAATCTCTTCGCACTCTACGATGCACAGGCCAAGGTCTGGCTGCCCCCAAACC ACGTTCTGGACACATCCCAAGACGCAACCCTGAGGCTCTATTTTCGAATGAGGTTTTACTTCCGGAACTG GCATGGCATGAATCCCCAAGAGCCTGCTGTATACCGATGTGGTTTCCCAGGGGCAGAGACTTCCTCAGAC CATGCAGAGCAAGGTGTACAGCTCTTGGACTCTGCCTCATTTGAATACCTTTTTGAGCAGGGAAAACATG AATTCATGAACGATGTGGTATCTCTGCGGGACCTGTCTAGCGAGGAAGAGATCCACCACTTTAAGAATGA GAGCCTGGGCATGGCCTTTCTGCACCTCTGCCATCTTGCTCTCAACCGTGGCGTCCCCCTGGAGGAGATG GCCAGAGAGATCAGCTTCAAGAACTGTATCCCTCACTCCTTCCGTCAGCACATCCGCCAGCACAATGTGC TTACGCGCCTGCGTCTCCGCAGAGTCTTCCGCCGCTTCCTGCAGGCCTTCCAGCCTGGCCACCTCTCCCA ACAGGTTGTGATGGTTAAGTACTTGGCCACCCTGGAGCGGCTGGCTCCTCGTTTTGGCTCAGAGCACATA CCTGTGTGTCATCTGGAAGTTCTGGCCCAGCCTGAGAGGGACCCCTGCTACATCCAGAACAGTGGGCAGA CCACTGGGGACCCAGGCCCAGAGCTGGCTTCTGGGCCAGCCACCCATGAGGTACTGGTGACAGGCACCGG AGGTATCCAGTGGCGTCCACTACAGACCCAGGACTCTGAGAGAGGTCACAGCAGAGGGAATCCCCAAGGC AGCCAGTCTGGGAAGAAACCCAAGGCCCCTGAGTCTGGAGAGCACCTGGCAGGGAGTCCCCAGGAACCAC CTTGGACCTACTTCTGTGACTTCCAGGACATTTCCCATGTGGTACTAAAGGAGCGTCGAGTGCACATCCA CCTTCAAGACAACAAGTGCTTGTTGCTGTGCCTCTGCTCCCAGGCCGAGGCCCTGTCCTTTGTGGCCCTG GTCGATGGCTATTTCCGCTTGACTGCTGACTCCAGCCACTACCTGTGCCATGAGGTAGCACCCCCGAGGC TGGTGACTAGCATCCAGAATGGCATCCACGGGCCCCTGATGGACCCATTTGTACAGGCTAAGCTGTGGCC AGAGGATGGCCTCTACCTGATCCAGTGGAGCACCAGCCACCTGCACCGACTGATCCTCACCGTAGCCCAT CGAAACCCGGCTTCCAGAAACGGCCCTAGGGGCCTACGCCTCCGAAAGTTCCCCATCACCCAGCAGCCTG GAGCCTTTGTGCTTGATGGTTGGGGCCGCTCCTTTGACAGCTTGGGGGACCTTCGGCAGGCCTTGCAGGG CTGCTCATTGCGAGCTGGTGATGACTGTTTCCCGTTGCACCACTGCTGCCTGCCCCGACCAAGAGAAATC TCCAACCTTGTCATCATGCGGGGGTCTAGGGCCCACACCCGGCCACTCAACCTCAGTCAGCTTAGCTTCC ACAGGGTTCACCAGGATGAAATCACCCAGCTGTCCCACTTGGGCCAAGGCACAAGGACCAATGTGTATGA GGGCCTGCTAAGTGTGGGAGGCCCCAATGAGGGCAAAGTGGACAGTGGATGCCCCTCTGGGCCTGATAGG GGCAGTGGGCAGCAGCTTCGAGTGGTGCTCAAAGTCCTCGACCCTAGTCACCATGATATCGCCTTGGCCT TCTATGAGACTGCCAGCCTCATGAGCCAGGTGTCACACATGCACCTGGCTTTCCTGCATGGTGTCTGCGT GCGTGGCTCAGAGAATATCATTGTAGCGGAATTCGTAGAGCACGGTCCCCTGGATGTGTGGTTACGGAGA CAGAGAGGCCGAGTGCCCATGCCCTGGAAGATGGTCGTGGCTCAGCAGCTGGCCAGCGCCCTGAGCTACC TGGAGGACAAGAATCTGGTTCACGGGAACGTATGTGGCCGGAACATCCTGCTGGCCCGCCTGGGGCTGGA GGAGGGTACCAACCCCTTCATCAAGCTCAGTGACCCTGGCGTGGGCCAAGGCGCCCTCTCCAGGGAAGAG CGGGTGGAGCGAATCCCCTGGACAGCTCCGGAGTGCCTGTCCGGAGGGGCCAACAGCTTGGGCACTGCCA CGGACATGTGGGGCTTTGGTGCCACCCTCCTTGAGATCTGTTTTGATGGAGAGGCACCTCTGCAGGGCCG TGGTCCCTCCGAGAAAGAACGGTTCTACACAAAGAAACATCAGCTGCCAGAGCCCTCATGCCCGGAGCTG GCCACACTCACCCGTCAGTGCCTGAGCTATGAACCCGCACAGAGGCCATCGTTCCGCACCATTTTGCGGG ACCTCACAAGACTGCAGCCACAGAATCTAGTGGGAACTTCGACTGTGAACTCGGACTCACCAGCATCGGA CCCCACCGTTTTTCACAAGCGTTACTTGAAAAGGATCCGGGATTTGGGTGAGGGTCACTTTGGCAAGGTC AGCCTGTACTGCTATGACCCGAACAATGACGGCACTGGCGAGATGGTGGCTGTGAAAGCCCTGAAGGAGG GATGTGGTCCCCAGCTCCGCTCAGGCTGGCAGCGGGAGATCGAAATCCTTCGGACGCTGTACCACGAGCA CATTGTCAAGTACAAAGGCTGCTGTGAAGACCAAGGGGAGAAGTCTGTACAGCTGGTCATGGAATACGTC CCTCTGGGCAGCCTCCGAGACTACCTGCCAAGGCACAGCGTGGGGCTGGCTCAGCTCCTGTTGTTTGCCC AGCAGATCTGCGAGGGCATGGCTTACCTGCACGCTCAGCACTACATTCACCGAGACCTCGCTGCGCGAAA CGTGCTGCTGGACAACGACCGGCTGGTCAAGATTGGAGACTTTGGCCTAGCCAAGGCTGTACCTGAAGGC CACGAGTACTACCGAGTGCGCGAGGACGGGGACAGCCCAGTATTCTGGTATGCCCCAGAATGCCTAAAGG AGTGCAAGTTTTACTATGCATCTGATGTCTGGTCCTTCGGGGTGACCCTTTATGAGCTGTTGACATACTG TGACTCTAGCCAGAGTCCCCCCACGAAATTCATCGAGCTCATCGGCGTAACCCAGGGCCAGATGACTGTG CTGAGGCTCACAGAGCTGCTGGAGCGAGGGGAGAGGCTGCCGCGGCCTGACAGATGTCCCTGTGAGATCT ATCACCTCATGAAGAACTGCTGGGAGTCAGAGGCCTCCTTCCGGCCCACGTTCCAGAATCTTGTGCCCAT CCTTAAGACAGCACAGGAGAAGTACCAAGGCCAGGTGCCTTCAGTGTTCAGCGTGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217534 representing NM_001257347 Red=Cloning site Green=Tags(s) MVTSMPLCGRRAASEDSKADGTEAQPLVPTGRLMVLLHWPGPEGGEPWVTFSQPSLTAEEMCIHIAHKVG ITPPCLNLFALYDAQAKVWLPPNHVLDTSQDATLRLYFRMRFYFRNWHGMNPQEPAVYRCGFPGAETSSD HAEQGVQLLDSASFEYLFEQGKHEFMNDVVSLRDLSSEEEIHHFKNESLGMAFLHLCHLALNRGVPLEEM AREISFKNCIPHSFRQHIRQHNVLTRLRLRRVFRRFLQAFQPGHLSQQVVMVKYLATLERLAPRFGSEHI PVCHLEVLAQPERDPCYIQNSGQTTGDPGPELASGPATHEVLVTGTGGIQWRPLQTQDSERGHSRGNPQG SQSGKKPKAPESGEHLAGSPQEPPWTYFCDFQDISHVVLKERRVHIHLQDNKCLLLCLCSQAEALSFVAL VDGYFRLTADSSHYLCHEVAPPRLVTSIQNGIHGPLMDPFVQAKLWPEDGLYLIQWSTSHLHRLILTVAH RNPASRNGPRGLRLRKFPITQQPGAFVLDGWGRSFDSLGDLRQALQGCSLRAGDDCFPLHHCCLPRPREI SNLVIMRGSRAHTRPLNLSQLSFHRVHQDEITQLSHLGQGTRTNVYEGLLSVGGPNEGKVDSGCPSGPDR GSGQQLRVVLKVLDPSHHDIALAFYETASLMSQVSHMHLAFLHGVCVRGSENIIVAEFVEHGPLDVWLRR QRGRVPMPWKMVVAQQLASALSYLEDKNLVHGNVCGRNILLARLGLEEGTNPFIKLSDPGVGQGALSREE RVERIPWTAPECLSGGANSLGTATDMWGFGATLLEICFDGEAPLQGRGPSEKERFYTKKHQLPEPSCPEL ATLTRQCLSYEPAQRPSFRTILRDLTRLQPQNLVGTSTVNSDSPASDPTVFHKRYLKRIRDLGEGHFGKV SLYCYDPNNDGTGEMVAVKALKEGCGPQLRSGWQREIEILRTLYHEHIVKYKGCCEDQGEKSVQLVMEYV PLGSLRDYLPRHSVGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEG HEYYRVREDGDSPVFWYAPECLKECKFYYASDVWSFGVTLYELLTYCDSSQSPPTKFIELIGVTQGQMTV LRLTELLERGERLPRPDRCPCEIYHLMKNCWESEASFRPTFQNLVPILKTAQEKYQGQVPSVFSVC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001257347
ORF Size	3558 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001257347.1, NP_001244276.1
RefSeq Size	4535 bp
RefSeq ORF	3561 bp
Locus ID	100361294
MW	133.3 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.