Thbs4 (NM_017133) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217365 representing NM_017133 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCCCCACGCGCGGCCGCCTTCCTCCTACTGCACCTGGTCCTGCAGCCGTGGCAGCGGGCGGGCG CCCAGGCCACACCCCAGGTCTTTGACCTTCTGCCATCCTCCAGCCAGAGGCTGAACCCGGCCGCCCTGCA GCCAGTCCTGACAGACCCCACCCTGCACGAGCTCTATGTCATCTCCACCTTCAAGCTGCAGAGTAAGAGC TCAGCCACCATCTTTGGCCTCTACTCCTCGAGTGACAACAGCAAATACTTTGAATTCACTGTGATGGGAC GCCTGAACAAAGCCATCCTCCGTTACCTAAAGGATGATGGGAAGATTCATCTGGTGGTTTTCAACAACCT GCAGCTGGCGGATGGGAGGCGCCACCGGATTCTCCTGAGACTCAGCAATTTGCAGAGAGGGGCCGGCTCC GTAGAACTCTACTTGGACTGTGTCCAGGTTGATTCAGTGAACAACCTCCCCAGAGCCTTCTCTGGTCTCA CCCAGAACCCACAGGCCATTGAATTGAGAACATTCCAGAGGAAGCCCCAGGACTTCTTGGAAGAGCTGAA GCTGGTGGTGAGGGGCTCTCTGTTCCAGGTGGCCAGCCTACAAGACTGTTTCCTGCAGCAGAGTGAGCCG CTGGCTGCCACAGGCACAGGAGACTTTAATCGACAGTTTTTGGGTCAAATGACACAATTAAACCAGCTCC TTGGAGAGGTGAAAGATCTTCTGAGGCAGCAGGTCAAGGAAACATCATTTTTGCGAAACACTATTGCTGA GTGCCAGGCTTGTGGCCCTCTCAGCTTCCAGTCCCCAACCCCAAACACGCTGGTGCCCATAGCTCCTCCA GCACCCCCAACCCGACCAACACGTCGCTGTGACTCCAGCCCTTGTTTCCGAGGTGTCCGATGCACGGACA CCAGGGACGGCTTCCAATGTGGACCCTGCCCAGATGGCTACACGGGGAACGGGATCACCTGTTCTGACGT GGATGAGTGTAAATACCATCCCTGCTACCCTGGTGTGCGCTGCACAAATTTGGCTCCGGGTTTCAGATGT GATGCCTGTCCAGTAGGCTTCACGGGGCCCATGGTGCAGGGCGTGGGGATCAACTTTGCCAAAACAAACA AGCAGGTGTGCACTGATGTTGACGAGTGTCGGAACGGAGCGTGTGTTCTGAACTCCATCTGCATTAACAC TCTGGGCTCTTACCGCTGTGGGCCTTGCAAGCCAGGGTACACTGGTGACCAGACGAGGGGATGCAGGACC GAAAGGAGCTGCCGGAACCCAGAGCAGAACCCGTGCAGTGTGCATGCGCAGTGCATTGAGGAACGGCAAG GGGATGTGACGTGTGTGTGCGGTGTTGGTTGGGCCGGCGACGGCTATGTCTGTGGAAAGGATGTGGACAT CGACAGTTACCCCGATGAAGAACTGCCGTGCTCAGCCAGGAACTGCAAGAAGGATAACTGCAAGTATGTG CCAAACTCCGGCCAAGAAGACGCAGACAGAGATGGCATTGGAGACGCCTGTGATGAGGATGCGGATGGAG ACGGGATCTTGAATGAGCAGGACAACTGTGTCCTGACTCACAATGTGGACCAGAGGAACACTGATAAAGA TATCTTTGGGGATGCCTGTGACAACTGCCGGGGCGTCCTGAATAATGACCAGAAGGACACTGACGGGGAT GGGAAAGGAGACGCCTGTGATGACGACATGGATGGAGACGGAATAAAAAACATTTTGGACAACTGTCCCC GAGTTCCCAACCGCGACCAACAGGATCGGGATGGCGATGGTGTCGGAGACGCCTGTGACAGCTGTCCTGA TGTCAGTAACCCTAACCAGTCGGATGTGGATAACGATCTGGTTGGGGACTCCTGTGACACCAACCAAGAC AGTGATGGGGATGGCCACCAGGACAGCACAGACAACTGCCCCACCGTCATTAACAGCGCCCAGCTGGACA CCGACAAGGACGGGATTGGCGATGAATGCGATGACGATGACGATAATGACGGCATCCCAGACCTGGTTCC CCCTGGCCCAGACAACTGCAGGCTTGTTCCCAACCCAGCCCAGGAAGATAGCAACAATGATGGAGTAGGA GATATCTGTGAAGCTGACTTCGACCAGGACAAGGTCATTGATCGCATTGATGTGTGTCCTGAGAATGCGG AGATCACCCTGACTGACTTCAGGGCCTATCAGACTGTGGTCCTGGACCCTGAAGGGGATGCCCAGATCGA CCCCAACTGGGTGGTCCTGAACCAGGGCATGGAGATTGTGCAGACCATGAACAGTGACCCTGGCCTAGCA GTCGGGTACACGGCATTTAATGGAGTTGACTTTGAAGGGACCTTCCACGTAAACACCCAGACAGATGACG ACTATGCCGGCTTTATTTTTGGTTACCAAGACAGTTCCAGCTTCTATGTGGTCATGTGGAAACAGACAGA GCAGACATACTGGCAGGCTACGCCATTCCGTGCAGTAGCAGAACCTGGTATTCAGCTCAAGGCCGTGAAG TCTAAAACTGGCCCAGGGGAACATCTCCGAAACTCCCTGTGGCACACAGGGGACACCAGCGACCAGGTCA GGCTGCTGTGGAAGGACTCCAGGAATGTGGGCTGGAAGGACAAGGTGTCCTACCGCTGGTTCCTGCAGCA TAGGCCCCAGGTGGGCTACATCAGGGTGCGGTTTTATGAAGGCTCAGAGTTGGTGGCTGACTCCGGGGTC ACTATAGACACCACCATGCGCGGAGGCCGACTGGGCGTGTTCTGCTTCTCTCAGGAAAACATCATCTGGT CCAACCTCAAGTACCGCTGTAACGACACCATACCAGAGGACTTCCAGGAGTTCCAGATCCAGACCTTTGA CCGCCTGGATAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017133
Insert Size	2886 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017133.1, NP_058829.1
RefSeq Size	3221 bp
RefSeq ORF	2886 bp
Locus ID	29220
Gene Summary	extracellular matrix protein; involved in neuronal development [RGD, Feb 2006]

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