Thbs4 (NM_017133) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217365 representing NM_017133 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAGCCCCACGCGCGGCCGCCTTCCTCCTACTGCACCTGGTCCTGCAGCCGTGGCAGCGGGCGGGCG CCCAGGCCACACCCCAGGTCTTTGACCTTCTGCCATCCTCCAGCCAGAGGCTGAACCCGGCCGCCCTGCA GCCAGTCCTGACAGACCCCACCCTGCACGAGCTCTATGTCATCTCCACCTTCAAGCTGCAGAGTAAGAGC TCAGCCACCATCTTTGGCCTCTACTCCTCGAGTGACAACAGCAAATACTTTGAATTCACTGTGATGGGAC GCCTGAACAAAGCCATCCTCCGTTACCTAAAGGATGATGGGAAGATTCATCTGGTGGTTTTCAACAACCT GCAGCTGGCGGATGGGAGGCGCCACCGGATTCTCCTGAGACTCAGCAATTTGCAGAGAGGGGCCGGCTCC GTAGAACTCTACTTGGACTGTGTCCAGGTTGATTCAGTGAACAACCTCCCCAGAGCCTTCTCTGGTCTCA CCCAGAACCCACAGGCCATTGAATTGAGAACATTCCAGAGGAAGCCCCAGGACTTCTTGGAAGAGCTGAA GCTGGTGGTGAGGGGCTCTCTGTTCCAGGTGGCCAGCCTACAAGACTGTTTCCTGCAGCAGAGTGAGCCG CTGGCTGCCACAGGCACAGGAGACTTTAATCGACAGTTTTTGGGTCAAATGACACAATTAAACCAGCTCC TTGGAGAGGTGAAAGATCTTCTGAGGCAGCAGGTCAAGGAAACATCATTTTTGCGAAACACTATTGCTGA GTGCCAGGCTTGTGGCCCTCTCAGCTTCCAGTCCCCAACCCCAAACACGCTGGTGCCCATAGCTCCTCCA GCACCCCCAACCCGACCAACACGTCGCTGTGACTCCAGCCCTTGTTTCCGAGGTGTCCGATGCACGGACA CCAGGGACGGCTTCCAATGTGGACCCTGCCCAGATGGCTACACGGGGAACGGGATCACCTGTTCTGACGT GGATGAGTGTAAATACCATCCCTGCTACCCTGGTGTGCGCTGCACAAATTTGGCTCCGGGTTTCAGATGT GATGCCTGTCCAGTAGGCTTCACGGGGCCCATGGTGCAGGGCGTGGGGATCAACTTTGCCAAAACAAACA AGCAGGTGTGCACTGATGTTGACGAGTGTCGGAACGGAGCGTGTGTTCTGAACTCCATCTGCATTAACAC TCTGGGCTCTTACCGCTGTGGGCCTTGCAAGCCAGGGTACACTGGTGACCAGACGAGGGGATGCAGGACC GAAAGGAGCTGCCGGAACCCAGAGCAGAACCCGTGCAGTGTGCATGCGCAGTGCATTGAGGAACGGCAAG GGGATGTGACGTGTGTGTGCGGTGTTGGTTGGGCCGGCGACGGCTATGTCTGTGGAAAGGATGTGGACAT CGACAGTTACCCCGATGAAGAACTGCCGTGCTCAGCCAGGAACTGCAAGAAGGATAACTGCAAGTATGTG CCAAACTCCGGCCAAGAAGACGCAGACAGAGATGGCATTGGAGACGCCTGTGATGAGGATGCGGATGGAG ACGGGATCTTGAATGAGCAGGACAACTGTGTCCTGACTCACAATGTGGACCAGAGGAACACTGATAAAGA TATCTTTGGGGATGCCTGTGACAACTGCCGGGGCGTCCTGAATAATGACCAGAAGGACACTGACGGGGAT GGGAAAGGAGACGCCTGTGATGACGACATGGATGGAGACGGAATAAAAAACATTTTGGACAACTGTCCCC GAGTTCCCAACCGCGACCAACAGGATCGGGATGGCGATGGTGTCGGAGACGCCTGTGACAGCTGTCCTGA TGTCAGTAACCCTAACCAGTCGGATGTGGATAACGATCTGGTTGGGGACTCCTGTGACACCAACCAAGAC AGTGATGGGGATGGCCACCAGGACAGCACAGACAACTGCCCCACCGTCATTAACAGCGCCCAGCTGGACA CCGACAAGGACGGGATTGGCGATGAATGCGATGACGATGACGATAATGACGGCATCCCAGACCTGGTTCC CCCTGGCCCAGACAACTGCAGGCTTGTTCCCAACCCAGCCCAGGAAGATAGCAACAATGATGGAGTAGGA GATATCTGTGAAGCTGACTTCGACCAGGACAAGGTCATTGATCGCATTGATGTGTGTCCTGAGAATGCGG AGATCACCCTGACTGACTTCAGGGCCTATCAGACTGTGGTCCTGGACCCTGAAGGGGATGCCCAGATCGA CCCCAACTGGGTGGTCCTGAACCAGGGCATGGAGATTGTGCAGACCATGAACAGTGACCCTGGCCTAGCA GTCGGGTACACGGCATTTAATGGAGTTGACTTTGAAGGGACCTTCCACGTAAACACCCAGACAGATGACG ACTATGCCGGCTTTATTTTTGGTTACCAAGACAGTTCCAGCTTCTATGTGGTCATGTGGAAACAGACAGA GCAGACATACTGGCAGGCTACGCCATTCCGTGCAGTAGCAGAACCTGGTATTCAGCTCAAGGCCGTGAAG TCTAAAACTGGCCCAGGGGAACATCTCCGAAACTCCCTGTGGCACACAGGGGACACCAGCGACCAGGTCA GGCTGCTGTGGAAGGACTCCAGGAATGTGGGCTGGAAGGACAAGGTGTCCTACCGCTGGTTCCTGCAGCA TAGGCCCCAGGTGGGCTACATCAGGGTGCGGTTTTATGAAGGCTCAGAGTTGGTGGCTGACTCCGGGGTC ACTATAGACACCACCATGCGCGGAGGCCGACTGGGCGTGTTCTGCTTCTCTCAGGAAAACATCATCTGGT CCAACCTCAAGTACCGCTGTAACGACACCATACCAGAGGACTTCCAGGAGTTCCAGATCCAGACCTTTGA CCGCCTGGATAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217365 representing NM_017133 Red=Cloning site Green=Tags(s) MLAPRAAAFLLLHLVLQPWQRAGAQATPQVFDLLPSSSQRLNPAALQPVLTDPTLHELYVISTFKLQSKS SATIFGLYSSSDNSKYFEFTVMGRLNKAILRYLKDDGKIHLVVFNNLQLADGRRHRILLRLSNLQRGAGS VELYLDCVQVDSVNNLPRAFSGLTQNPQAIELRTFQRKPQDFLEELKLVVRGSLFQVASLQDCFLQQSEP LAATGTGDFNRQFLGQMTQLNQLLGEVKDLLRQQVKETSFLRNTIAECQACGPLSFQSPTPNTLVPIAPP APPTRPTRRCDSSPCFRGVRCTDTRDGFQCGPCPDGYTGNGITCSDVDECKYHPCYPGVRCTNLAPGFRC DACPVGFTGPMVQGVGINFAKTNKQVCTDVDECRNGACVLNSICINTLGSYRCGPCKPGYTGDQTRGCRT ERSCRNPEQNPCSVHAQCIEERQGDVTCVCGVGWAGDGYVCGKDVDIDSYPDEELPCSARNCKKDNCKYV PNSGQEDADRDGIGDACDEDADGDGILNEQDNCVLTHNVDQRNTDKDIFGDACDNCRGVLNNDQKDTDGD GKGDACDDDMDGDGIKNILDNCPRVPNRDQQDRDGDGVGDACDSCPDVSNPNQSDVDNDLVGDSCDTNQD SDGDGHQDSTDNCPTVINSAQLDTDKDGIGDECDDDDDNDGIPDLVPPGPDNCRLVPNPAQEDSNNDGVG DICEADFDQDKVIDRIDVCPENAEITLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLA VGYTAFNGVDFEGTFHVNTQTDDDYAGFIFGYQDSSSFYVVMWKQTEQTYWQATPFRAVAEPGIQLKAVK SKTGPGEHLRNSLWHTGDTSDQVRLLWKDSRNVGWKDKVSYRWFLQHRPQVGYIRVRFYEGSELVADSGV TIDTTMRGGRLGVFCFSQENIIWSNLKYRCNDTIPEDFQEFQIQTFDRLDN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_017133
ORF Size	2883 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_017133.1, NP_058829.1
RefSeq Size	3221 bp
RefSeq ORF	2886 bp
Locus ID	29220
MW	106 kDa
Gene Summary	extracellular matrix protein; involved in neuronal development [RGD, Feb 2006]

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