Dgkq (NM_001198804) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Dgkq
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217349 representing NM_001198804 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCAGCCGAGTCCGGGGCCCGGACCTGGCCGGGCAGTGGTTCCCCACGTCTCGGCAGCCCGG CCGGCAGCCCTGTGCTGGGCATCTCGGGCCGCGCACGCCCGGGGTCAGGGCCGGAGCGGACTGGCAGAGC TATCGGCTCCGTAGCACCCGGCCACAGCTTTCGCAAGGTGACACTCACCAAGCCTACCTTCTGCCACCTC TGCTCGGACTTCATCTGGGGATTGGCCGGCTTCCTGTGCGATGTCTGCAACTTCATGTCCCATGAGAAGT GCCTAAAGCAGGTGAAGACCCCGTGCACAAGCATTGCGCCAAGCCTCGTCCGGGTCCCTGTAGCCCACTG CTTTGGTTCCCTTGGTCTCTACAAGCGCAAGTTCTGTGTGGTCTGCCGCAAGAGCCTGGAGGTACCTGCG TTCCGCTGTGAAGTGTGTGAGCTGCACGTTCACCCCGACTGTGTGCCCTTCGCCTGCAGCGACTGTCGTC AGTGTCACCAGGATGGACAGCACGATTATGACACGTATCACCACCACTGGAGGGAGGGGAATCTGCCTTC TGGTGCTCGATGTGAGGTCTGTAGGAAGACTTGTGGCTCCTCGGATGTGCTGGCTGGTGTACGCTGCGAG TGGTGTGGTGTACAGGCTCACTCCGTGTGCTCCACAGCACTCACCCCTGAGTGTACATTTGGACGTCTAC GCTCCATGGTACTGCCTCCTTCGTGTGTGCGCCTGTTGTCCCGAAACTTCAGCAAGATGCACTGCTTCCG AATCCCCGAGACCATGGTCCTGGAGCTCGGTGATGGGGATGATGGCCTAGATGGGAGTGCTGCAGTCGGC ACAGGCAGAGAGGTATCGGCAGCTACAGAATCTACCAAACAGACCCTGAAGATCTTTGATGGCAACGACT CCATGAGGAAAAATCAGTTTCGCCTGGTTACAGTTTCCCGCCTAGCTCGGAATGAGGAAGTGATGGAGGC AGCACTCCGGGCCTACTATATCAACGAGGACCCTAAGGACTTCCAGCTGCAGGCACTGCCCCTAACGTTA CTGTCTGGCAATGCCCAGGCTCTGGGGAAGGCCGGGACCACTGAGGAGGAGACAAGTAAAGACTCCGGGC CCGGGGATTCCGTGCCTGAGGCCTGGGTCATCAGGTCTCTGCCTCGTACCCAAGAGATCCTGAAGATCTA CCCTGACTGGCTCAAGGTAGGTGTGGCCTACGTGTCCATCCGTGTGAACTCCCAGAGTACAGCACGGTCT GTGGTTCAAGAGGTTCTCCCACTGTTTGGACGACAGGTTGAGGATCAGGAGAGATTCCAGCTGATTGAGG TGCTTATGAGCAGCAGACAAGTCCAACGGACGGTGCTGGTGGATGAAGAACCTCTGCTAGATCGACTCCG GGACATCCGACAGACATCTGTGCGCCAGGCGAGCCAGACGCGGTTCTATGTGGCCGAGGCCAGGGCCGTG ACCCCACATGTCTCCCTGTTTGTGGGTGGCCTGCCACCTGGCTTGTCCCCTCAGGATTACAGCAACCTGC TGCATGAGGCCATGGCCACCAAAGCTGCTGTGGTGTCTGTGAGTCACGTCTACTCCTTACAAGGTGCGGT AGTTCTGGATGTCACCTGCTTCGCGGAGGCTGAGCGGCTATACATGCTGGCCAGGGACACAGCAGTGCAT GGCCGGCCACTGACTGCACTAGTGCTTCCAGATGTGCTGCACACGAAGCTGCCTCCTGACTGCTGCCCTC TCCTCGTGTTTGTGAACCCCAAGAGTGGGGGCCTCAAGGGACGAGAACTGCTCTGCAGTTTCCGGAAGCT GCTGAATCCACACCAGGTCTTTGAGCTCACCAACGGGGGCCCTCTTCCTGGGTTCCACCTTTTCTCCCAG GTGCCCTGCTTTCGGGTACTGGTCTGTGGTGGAGATGGCACCGTGGGCTGGGTGCTCGCTGCCCTGGAGG AGACAAGGCGCCATCTGGCCTGCCCAGAGCCATCTGTGGCCATCCTACCCCTGGGTACAGGGAATGACCT TGGCCGGGTCCTCCGTTGGGGAGCAGGCTATAGTGGTGAGGACCCATTTTCTGTGCTGGTGTCTGTGGAT GAGGCTGATGCTGTGCTCATGGATCGATGGACAATCCTGCTGGACGCTCATGAAATTGATAGTACAGAGA ACAATGTGGTAGAAACAGAGCCCCCCAAGATTGTTCAGATGAATAACTACTGTGGCATTGGCATTGATGC GGAGCTCAGCCTAGACTTCCACCAGGCACGTGAAGAGGAGCCTGGCAAATTCACAAGCAGGTTCCACAAC AAGGGCGTGTATGTGCGGGTTGGGCTGCAGAAGATCAGCCACTCTCGAAGCCTGCACAAGGAGATCCGTC TGCAGGTGGAGCAGCAGGAAGTGGAGCTGCCCAGCATTGAGGGTCTTATCTTCATTAACATCCCCAGCTG GGGCTCAGGGGCTGACCTGTGGGGCTCTGACAGTGACTCAAGGTTTGAGAAGCCACGCATAGACGACGGG CTGTTGGAGGTGGTGGGTGTGACAGGTGTCGTGCACATGGGCCAGGTACAAGGTGGGCTGCGCTCTGGAA TCCGAATCGCCCAGGGCTCCTACTTCCGTGTCACACTCCTCAAGGCTACTCCAGTGCAGGTGGACGGTGA GCCCTGGATTCAGGCCCCAGGTCACATGATCATCTCTGCTACTGCACCTAAGGTTCACATGCTGAGGAAA GCTAAGCAGAAGCCCAGGAAGGCTGGCGCCATCAGGGATACCCGAGTGGACACCTTGCCTGCTCCTGAGG GCAATCCTTTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198804
Insert Size	2814 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198804.1, NP_001185733.1
RefSeq Size	4536 bp
RefSeq ORF	2814 bp
Locus ID	100361138
UniProt ID	D3ZEY4
Gene Summary	Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15337525). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15337525). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (By similarity). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (PubMed:15337525). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]

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