Dgkq (NM_001198804) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Dgkq
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217349 representing NM_001198804 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACGGCAGCCGAGTCCGGGGCCCGGACCTGGCCGGGCAGTGGTTCCCCACGTCTCGGCAGCCCGG CCGGCAGCCCTGTGCTGGGCATCTCGGGCCGCGCACGCCCGGGGTCAGGGCCGGAGCGGACTGGCAGAGC TATCGGCTCCGTAGCACCCGGCCACAGCTTTCGCAAGGTGACACTCACCAAGCCTACCTTCTGCCACCTC TGCTCGGACTTCATCTGGGGATTGGCCGGCTTCCTGTGCGATGTCTGCAACTTCATGTCCCATGAGAAGT GCCTAAAGCAGGTGAAGACCCCGTGCACAAGCATTGCGCCAAGCCTCGTCCGGGTCCCTGTAGCCCACTG CTTTGGTTCCCTTGGTCTCTACAAGCGCAAGTTCTGTGTGGTCTGCCGCAAGAGCCTGGAGGTACCTGCG TTCCGCTGTGAAGTGTGTGAGCTGCACGTTCACCCCGACTGTGTGCCCTTCGCCTGCAGCGACTGTCGTC AGTGTCACCAGGATGGACAGCACGATTATGACACGTATCACCACCACTGGAGGGAGGGGAATCTGCCTTC TGGTGCTCGATGTGAGGTCTGTAGGAAGACTTGTGGCTCCTCGGATGTGCTGGCTGGTGTACGCTGCGAG TGGTGTGGTGTACAGGCTCACTCCGTGTGCTCCACAGCACTCACCCCTGAGTGTACATTTGGACGTCTAC GCTCCATGGTACTGCCTCCTTCGTGTGTGCGCCTGTTGTCCCGAAACTTCAGCAAGATGCACTGCTTCCG AATCCCCGAGACCATGGTCCTGGAGCTCGGTGATGGGGATGATGGCCTAGATGGGAGTGCTGCAGTCGGC ACAGGCAGAGAGGTATCGGCAGCTACAGAATCTACCAAACAGACCCTGAAGATCTTTGATGGCAACGACT CCATGAGGAAAAATCAGTTTCGCCTGGTTACAGTTTCCCGCCTAGCTCGGAATGAGGAAGTGATGGAGGC AGCACTCCGGGCCTACTATATCAACGAGGACCCTAAGGACTTCCAGCTGCAGGCACTGCCCCTAACGTTA CTGTCTGGCAATGCCCAGGCTCTGGGGAAGGCCGGGACCACTGAGGAGGAGACAAGTAAAGACTCCGGGC CCGGGGATTCCGTGCCTGAGGCCTGGGTCATCAGGTCTCTGCCTCGTACCCAAGAGATCCTGAAGATCTA CCCTGACTGGCTCAAGGTAGGTGTGGCCTACGTGTCCATCCGTGTGAACTCCCAGAGTACAGCACGGTCT GTGGTTCAAGAGGTTCTCCCACTGTTTGGACGACAGGTTGAGGATCAGGAGAGATTCCAGCTGATTGAGG TGCTTATGAGCAGCAGACAAGTCCAACGGACGGTGCTGGTGGATGAAGAACCTCTGCTAGATCGACTCCG GGACATCCGACAGACATCTGTGCGCCAGGCGAGCCAGACGCGGTTCTATGTGGCCGAGGCCAGGGCCGTG ACCCCACATGTCTCCCTGTTTGTGGGTGGCCTGCCACCTGGCTTGTCCCCTCAGGATTACAGCAACCTGC TGCATGAGGCCATGGCCACCAAAGCTGCTGTGGTGTCTGTGAGTCACGTCTACTCCTTACAAGGTGCGGT AGTTCTGGATGTCACCTGCTTCGCGGAGGCTGAGCGGCTATACATGCTGGCCAGGGACACAGCAGTGCAT GGCCGGCCACTGACTGCACTAGTGCTTCCAGATGTGCTGCACACGAAGCTGCCTCCTGACTGCTGCCCTC TCCTCGTGTTTGTGAACCCCAAGAGTGGGGGCCTCAAGGGACGAGAACTGCTCTGCAGTTTCCGGAAGCT GCTGAATCCACACCAGGTCTTTGAGCTCACCAACGGGGGCCCTCTTCCTGGGTTCCACCTTTTCTCCCAG GTGCCCTGCTTTCGGGTACTGGTCTGTGGTGGAGATGGCACCGTGGGCTGGGTGCTCGCTGCCCTGGAGG AGACAAGGCGCCATCTGGCCTGCCCAGAGCCATCTGTGGCCATCCTACCCCTGGGTACAGGGAATGACCT TGGCCGGGTCCTCCGTTGGGGAGCAGGCTATAGTGGTGAGGACCCATTTTCTGTGCTGGTGTCTGTGGAT GAGGCTGATGCTGTGCTCATGGATCGATGGACAATCCTGCTGGACGCTCATGAAATTGATAGTACAGAGA ACAATGTGGTAGAAACAGAGCCCCCCAAGATTGTTCAGATGAATAACTACTGTGGCATTGGCATTGATGC GGAGCTCAGCCTAGACTTCCACCAGGCACGTGAAGAGGAGCCTGGCAAATTCACAAGCAGGTTCCACAAC AAGGGCGTGTATGTGCGGGTTGGGCTGCAGAAGATCAGCCACTCTCGAAGCCTGCACAAGGAGATCCGTC TGCAGGTGGAGCAGCAGGAAGTGGAGCTGCCCAGCATTGAGGGTCTTATCTTCATTAACATCCCCAGCTG GGGCTCAGGGGCTGACCTGTGGGGCTCTGACAGTGACTCAAGGTTTGAGAAGCCACGCATAGACGACGGG CTGTTGGAGGTGGTGGGTGTGACAGGTGTCGTGCACATGGGCCAGGTACAAGGTGGGCTGCGCTCTGGAA TCCGAATCGCCCAGGGCTCCTACTTCCGTGTCACACTCCTCAAGGCTACTCCAGTGCAGGTGGACGGTGA GCCCTGGATTCAGGCCCCAGGTCACATGATCATCTCTGCTACTGCACCTAAGGTTCACATGCTGAGGAAA GCTAAGCAGAAGCCCAGGAAGGCTGGCGCCATCAGGGATACCCGAGTGGACACCTTGCCTGCTCCTGAGG GCAATCCTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217349 representing NM_001198804 Red=Cloning site Green=Tags(s) MATAAESGARTWPGSGSPRLGSPAGSPVLGISGRARPGSGPERTGRAIGSVAPGHSFRKVTLTKPTFCHL CSDFIWGLAGFLCDVCNFMSHEKCLKQVKTPCTSIAPSLVRVPVAHCFGSLGLYKRKFCVVCRKSLEVPA FRCEVCELHVHPDCVPFACSDCRQCHQDGQHDYDTYHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCE WCGVQAHSVCSTALTPECTFGRLRSMVLPPSCVRLLSRNFSKMHCFRIPETMVLELGDGDDGLDGSAAVG TGREVSAATESTKQTLKIFDGNDSMRKNQFRLVTVSRLARNEEVMEAALRAYYINEDPKDFQLQALPLTL LSGNAQALGKAGTTEEETSKDSGPGDSVPEAWVIRSLPRTQEILKIYPDWLKVGVAYVSIRVNSQSTARS VVQEVLPLFGRQVEDQERFQLIEVLMSSRQVQRTVLVDEEPLLDRLRDIRQTSVRQASQTRFYVAEARAV TPHVSLFVGGLPPGLSPQDYSNLLHEAMATKAAVVSVSHVYSLQGAVVLDVTCFAEAERLYMLARDTAVH GRPLTALVLPDVLHTKLPPDCCPLLVFVNPKSGGLKGRELLCSFRKLLNPHQVFELTNGGPLPGFHLFSQ VPCFRVLVCGGDGTVGWVLAALEETRRHLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLVSVD EADAVLMDRWTILLDAHEIDSTENNVVETEPPKIVQMNNYCGIGIDAELSLDFHQAREEEPGKFTSRFHN KGVYVRVGLQKISHSRSLHKEIRLQVEQQEVELPSIEGLIFINIPSWGSGADLWGSDSDSRFEKPRIDDG LLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWIQAPGHMIISATAPKVHMLRK AKQKPRKAGAIRDTRVDTLPAPEGNPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198804
ORF Size	2811 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198804.1, NP_001185733.1
RefSeq Size	4536 bp
RefSeq ORF	2814 bp
Locus ID	100361138
UniProt ID	D3ZEY4
MW	102.5 kDa
Gene Summary	Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15337525). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:15337525). Within the adrenocorticotropic hormone signaling pathway, produces phosphatidic acid which in turn activates NR5A1 and subsequent steroidogenic gene transcription (By similarity). Also functions downstream of the nerve growth factor signaling pathway being specifically activated in the nucleus by the growth factor (PubMed:15337525). Through its diacylglycerol activity also regulates synaptic vesicle endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]

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