Hcn4 (NM_021658) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214763 representing NM_021658 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGCTGCCGCCGTCCATGCGCAAGCGGCTCTACAGCCTTCCGCAGCAGGTGGGGGCCAAGGCGT GGATCATGGACGAGGAAGAGGATGGTGAGGAAGAGGGGGCCGGGGGCCTCCAGGACCCAAGCCGAAGGAG CATTCGGCTGCGGCCGCTGCCCTCGCCCTCGCCCTCGGTGGCCGCCGGCTGCTCCGAGTCCCGGGGTGCG GCCCTCGGGGCGGCAGACAGCGAGGGGCCGGGCCGCAGCGCCGGCAAGTCCAGCACCAACGGTGACTGCA GGCGCTTCCGCGGGAGTCTGGCCTCGCTGGGCAGCCGGGGCGGCGGCAGTGGTGGAGCAGGGGGCGGCAG CAGTCTCGGGCACCTGCATGACTCCGCGGAGGAGCGGCGGCTCATCGCCGCTGAGGGCGATGCGTCCCCC GGCGAGGACAGGACGCCCCCGGGCCTGGCGACCGAGCCCGAGCGCCCGGGCGCCGCGGCACAACCCGCAG CCTCGCCGCCGCCCCAACAGCCGCCGCAGCCGGCCTCCGCCTCCTGCGAGCAGCCCTCGGCGGACACAGC TATCAAAGTGGAAGGAGGCGCGGCCGCCAGCGACCAGATCCTCCCTGAGGCCGAGGTGCGCCTGGGCCAG AGCGGCTTCATGCAGCGCCAGTTCGGTGCCATGCTGCAACCTGGGGTCAACAAATTCTCGCTAAGGATGT TCGGCAGCCAGAAAGCAGTGGAGCGTGAGCAGGAGAGGGTTAAGTCAGCTGGGTTTTGGATTATCCACCC CTACAGCGACTTCAGATTTTACTGGGACCTGACGATGCTGTTGCTGATGGTGGGGAATCTGATCATCATC CCTGTGGGCATCACCTTCTTCAAGGATGAGAACACCACCCCCTGGATCGTCTTCAACGTGGTGTCAGACA CATTCTTCCTCATTGACTTGGTCCTCAACTTCCGCACGGGGATCGTGGTGGAGGACAACACAGAAATCAT CCTTGACCCACAGCGGATCAAGATGAAGTACCTGAAAAGCTGGTTTGTGGTGGACTTCATCTCCTCCATC CCCGTGGACTACATCTTCCTTATAGTGGAGACTCGCATTGACTCGGAGGTCTACAAAACCGCCAGGGCTC TGCGCATTGTCCGCTTCACGAAGATCCTCAGCCTCCTGCGCCTCCTGCGGCTTTCCCGCCTCATTCGGTA CATTCACCAGTGGGAAGAGATCTTCCACATGACCTACGACCTGGCCAGTGCCGTGGTACGCATCGTGAAC CTCATTGGCATGATGCTTCTGCTTTGCCACTGGGATGGCTGCCTGCAGTTCCTGGTGCCCATGCTGCAGG ACTTCCCCCATGACTGCTGGGTGTCCATCAACGGCATGGTGAATAACTCCTGGGGGAAGCAGTACTCCTA TGCCCTCTTCAAGGCCATGAGCCACATGCTGTGTATTGGGTACGGACGGCAGGCACCCGTAGGCATGTCT GACGTCTGGCTCACCATGCTCAGCATGATCGTGGGCGCCACCTGCTATGCCATGTTCATCGGGCACGCCA CTGCCCTCATCCAGTCGCTGGACTCCTCCCGGCGCCAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTA CATGTCCTTCCACAAGCTCCCGCCTGACACCAGGCAGCGCATCCACGACTACTACGAACACCGCTACCAG GGCAAGATGTTTGACGAGGAAAGCATCCTGGGTGAGCTGAGTGAGCCGCTTCGAGAGGAGATCATCAACT TTAACTGCCGGAAGCTGGTGGCATCCATGCCACTGTTCGCCAATGCAGACCCCAACTTTGTGACGTCTAT GCTGACCAAGTTGCGTTTTGAGGTCTTTCAGCCTGGGGACTACATCATCCGTGAAGGCACCATCGGCAAG AAGATGTACTTTATCCAGCACGGCGTGGTCAGTGTGCTCACTAAGGGCAACAAGGAGACCAAGCTGGCTG ATGGCTCCTATTTTGGAGAGATCTGCTTGCTGACCCGAGGCCGTCGCACAGCGAGCGTGAGGGCGGATAC TTACTGCCGCCTCTACTCACTGAGCGTGGACAACTTCAACGAGGTGCTGGAGGAGTATCCCATGATGCGC AGGGCTTTCGAGACGGTTGCGCTGGACCGTCTGGACCGCATAGGCAAGAAGAACTCCATCCTCCTCCACA AGGTGCAGCACGACCTCAACTCAGGCGTCTTCAACTACCAAGAGAACGAGATCATCCAGCAGATCGTGCG GCATGACCGTGAGATGGCCCACTGTGCTCACCGCGTCCAGGCTGCTGCCTCAGCCACCCCAACCCCAACG CCTGTCATATGGACCCCACTGATCCAGGCACCACTGCAGGCTGCTGCTGCTACTACTTCGGTGGCCATAG CCCTCACACACCACCCCCGCCTGCCAGCCGCTATCTTCCGGCCCCCTCCCGGACCTGGGCTGGGTAACCT GGGGGCTGGACAGACACCGAGGCACCCAAGGAGGTTGCAGTCCTTGATCCCTTCAGCGCTAGGCTCTGCT TCACCAGCCAGCAGCCCCTCACAGGTGGACACACCGTCTTCATCTTCCTTCCACATCCAACAGCTGGCTG GATTCTCTGCACCTCCTGGATTGAGTCCTCTCTTGCCCTCCTCTAGCTCTTCCCCACCTCCAGGAGCCTG CAGTTCTCCCCCAGCCCCCACTCCATCCACCTCCACTGCTGCCACCACCACCGGGTTCGGCCACTTTCAT AAGGCGCTAGGTGGCTCCCTGTCTTCCTCTGATTCCCCGCTGCTCACCCCACTGCAACCGGGCGCTCGCT CTCCACAGGCTGCCCAGCCGCCACCCCCACTGCCTGGGGCCCGAGGAGGCCTGGGACTCCTGGAGCACTT CTTGCCGCCCCCACCTTCGTCCCGGTCACCATCATCTAGCCCTGGGCAGCTGGGCCAGCCTCCTGGAGAG TTGTCCCCAGGTCTGGCAGCTGGTCCACCAAGTACACCAGAGACACCCCCGCGGCCCGAACGGCCATCCT TTATGGCAGGGGCCTCTGGGGGGGCTTCTCCTGTAGCCTTTACCCCCCGAGGAGGCCTCAGCCCTCCGGG CCACAGCCCAGGACCCCCAAGAACTTTCCCGAGTGCCCCACCCCGGGCCTCTGGCTCCCATGGTTCCCTG CTCCTGCCACCTGCATCCAGCCCTCCGCCTCCCCAGGTCCCACAGCGCAGGGGCACACCACCCCTCACCC CCGGCCGCCTCACACAGGACCTGAAGCTCATCTCAGCCTCTCAGCCAGCCCTCCCCCAGGATGGGGCACA GACTCTACGCAGGGCCTCTCCTCACTCCTCAGGGGAGTCGATGGCTGCCTTCTCACTCTACCCCAGAGCT GGGGGTGGCAGTGGGAGCAGTGGGGGCCTTGGGCCTCCTGGAAGGCCATATGGTGCCATCCCAGGCCAGC ATGTCACTTTGCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCACTTTCTTTGTTTGGGGCAAGAGC CGCCTCTTCTGGAGGGCCCCCTCTGACTGCTGCACCCCAGAGGGAACCTGGCGCTAGGTCCGAGCCAGTA CGCTCCAAACTGCCGTCTAATTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_021658
Insert Size	3597 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_021658.1, NP_067690.1
RefSeq Size	3971 bp
RefSeq ORF	3597 bp
Locus ID	59266
UniProt ID	Q9JKA7
Gene Summary	may act as a neuronal pacemaker channel; may play a role in neurotransmitter release [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.