Hcn4 (NM_021658) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214763 representing NM_021658 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGCTGCCGCCGTCCATGCGCAAGCGGCTCTACAGCCTTCCGCAGCAGGTGGGGGCCAAGGCGT GGATCATGGACGAGGAAGAGGATGGTGAGGAAGAGGGGGCCGGGGGCCTCCAGGACCCAAGCCGAAGGAG CATTCGGCTGCGGCCGCTGCCCTCGCCCTCGCCCTCGGTGGCCGCCGGCTGCTCCGAGTCCCGGGGTGCG GCCCTCGGGGCGGCAGACAGCGAGGGGCCGGGCCGCAGCGCCGGCAAGTCCAGCACCAACGGTGACTGCA GGCGCTTCCGCGGGAGTCTGGCCTCGCTGGGCAGCCGGGGCGGCGGCAGTGGTGGAGCAGGGGGCGGCAG CAGTCTCGGGCACCTGCATGACTCCGCGGAGGAGCGGCGGCTCATCGCCGCTGAGGGCGATGCGTCCCCC GGCGAGGACAGGACGCCCCCGGGCCTGGCGACCGAGCCCGAGCGCCCGGGCGCCGCGGCACAACCCGCAG CCTCGCCGCCGCCCCAACAGCCGCCGCAGCCGGCCTCCGCCTCCTGCGAGCAGCCCTCGGCGGACACAGC TATCAAAGTGGAAGGAGGCGCGGCCGCCAGCGACCAGATCCTCCCTGAGGCCGAGGTGCGCCTGGGCCAG AGCGGCTTCATGCAGCGCCAGTTCGGTGCCATGCTGCAACCTGGGGTCAACAAATTCTCGCTAAGGATGT TCGGCAGCCAGAAAGCAGTGGAGCGTGAGCAGGAGAGGGTTAAGTCAGCTGGGTTTTGGATTATCCACCC CTACAGCGACTTCAGATTTTACTGGGACCTGACGATGCTGTTGCTGATGGTGGGGAATCTGATCATCATC CCTGTGGGCATCACCTTCTTCAAGGATGAGAACACCACCCCCTGGATCGTCTTCAACGTGGTGTCAGACA CATTCTTCCTCATTGACTTGGTCCTCAACTTCCGCACGGGGATCGTGGTGGAGGACAACACAGAAATCAT CCTTGACCCACAGCGGATCAAGATGAAGTACCTGAAAAGCTGGTTTGTGGTGGACTTCATCTCCTCCATC CCCGTGGACTACATCTTCCTTATAGTGGAGACTCGCATTGACTCGGAGGTCTACAAAACCGCCAGGGCTC TGCGCATTGTCCGCTTCACGAAGATCCTCAGCCTCCTGCGCCTCCTGCGGCTTTCCCGCCTCATTCGGTA CATTCACCAGTGGGAAGAGATCTTCCACATGACCTACGACCTGGCCAGTGCCGTGGTACGCATCGTGAAC CTCATTGGCATGATGCTTCTGCTTTGCCACTGGGATGGCTGCCTGCAGTTCCTGGTGCCCATGCTGCAGG ACTTCCCCCATGACTGCTGGGTGTCCATCAACGGCATGGTGAATAACTCCTGGGGGAAGCAGTACTCCTA TGCCCTCTTCAAGGCCATGAGCCACATGCTGTGTATTGGGTACGGACGGCAGGCACCCGTAGGCATGTCT GACGTCTGGCTCACCATGCTCAGCATGATCGTGGGCGCCACCTGCTATGCCATGTTCATCGGGCACGCCA CTGCCCTCATCCAGTCGCTGGACTCCTCCCGGCGCCAGTACCAGGAGAAGTACAAGCAGGTGGAGCAGTA CATGTCCTTCCACAAGCTCCCGCCTGACACCAGGCAGCGCATCCACGACTACTACGAACACCGCTACCAG GGCAAGATGTTTGACGAGGAAAGCATCCTGGGTGAGCTGAGTGAGCCGCTTCGAGAGGAGATCATCAACT TTAACTGCCGGAAGCTGGTGGCATCCATGCCACTGTTCGCCAATGCAGACCCCAACTTTGTGACGTCTAT GCTGACCAAGTTGCGTTTTGAGGTCTTTCAGCCTGGGGACTACATCATCCGTGAAGGCACCATCGGCAAG AAGATGTACTTTATCCAGCACGGCGTGGTCAGTGTGCTCACTAAGGGCAACAAGGAGACCAAGCTGGCTG ATGGCTCCTATTTTGGAGAGATCTGCTTGCTGACCCGAGGCCGTCGCACAGCGAGCGTGAGGGCGGATAC TTACTGCCGCCTCTACTCACTGAGCGTGGACAACTTCAACGAGGTGCTGGAGGAGTATCCCATGATGCGC AGGGCTTTCGAGACGGTTGCGCTGGACCGTCTGGACCGCATAGGCAAGAAGAACTCCATCCTCCTCCACA AGGTGCAGCACGACCTCAACTCAGGCGTCTTCAACTACCAAGAGAACGAGATCATCCAGCAGATCGTGCG GCATGACCGTGAGATGGCCCACTGTGCTCACCGCGTCCAGGCTGCTGCCTCAGCCACCCCAACCCCAACG CCTGTCATATGGACCCCACTGATCCAGGCACCACTGCAGGCTGCTGCTGCTACTACTTCGGTGGCCATAG CCCTCACACACCACCCCCGCCTGCCAGCCGCTATCTTCCGGCCCCCTCCCGGACCTGGGCTGGGTAACCT GGGGGCTGGACAGACACCGAGGCACCCAAGGAGGTTGCAGTCCTTGATCCCTTCAGCGCTAGGCTCTGCT TCACCAGCCAGCAGCCCCTCACAGGTGGACACACCGTCTTCATCTTCCTTCCACATCCAACAGCTGGCTG GATTCTCTGCACCTCCTGGATTGAGTCCTCTCTTGCCCTCCTCTAGCTCTTCCCCACCTCCAGGAGCCTG CAGTTCTCCCCCAGCCCCCACTCCATCCACCTCCACTGCTGCCACCACCACCGGGTTCGGCCACTTTCAT AAGGCGCTAGGTGGCTCCCTGTCTTCCTCTGATTCCCCGCTGCTCACCCCACTGCAACCGGGCGCTCGCT CTCCACAGGCTGCCCAGCCGCCACCCCCACTGCCTGGGGCCCGAGGAGGCCTGGGACTCCTGGAGCACTT CTTGCCGCCCCCACCTTCGTCCCGGTCACCATCATCTAGCCCTGGGCAGCTGGGCCAGCCTCCTGGAGAG TTGTCCCCAGGTCTGGCAGCTGGTCCACCAAGTACACCAGAGACACCCCCGCGGCCCGAACGGCCATCCT TTATGGCAGGGGCCTCTGGGGGGGCTTCTCCTGTAGCCTTTACCCCCCGAGGAGGCCTCAGCCCTCCGGG CCACAGCCCAGGACCCCCAAGAACTTTCCCGAGTGCCCCACCCCGGGCCTCTGGCTCCCATGGTTCCCTG CTCCTGCCACCTGCATCCAGCCCTCCGCCTCCCCAGGTCCCACAGCGCAGGGGCACACCACCCCTCACCC CCGGCCGCCTCACACAGGACCTGAAGCTCATCTCAGCCTCTCAGCCAGCCCTCCCCCAGGATGGGGCACA GACTCTACGCAGGGCCTCTCCTCACTCCTCAGGGGAGTCGATGGCTGCCTTCTCACTCTACCCCAGAGCT GGGGGTGGCAGTGGGAGCAGTGGGGGCCTTGGGCCTCCTGGAAGGCCATATGGTGCCATCCCAGGCCAGC ATGTCACTTTGCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCACTTTCTTTGTTTGGGGCAAGAGC CGCCTCTTCTGGAGGGCCCCCTCTGACTGCTGCACCCCAGAGGGAACCTGGCGCTAGGTCCGAGCCAGTA CGCTCCAAACTGCCGTCTAATTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214763 representing NM_021658 Red=Cloning site Green=Tags(s) MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDGEEEGAGGLQDPSRRSIRLRPLPSPSPSVAAGCSESRGA ALGAADSEGPGRSAGKSSTNGDCRRFRGSLASLGSRGGGSGGAGGGSSLGHLHDSAEERRLIAAEGDASP GEDRTPPGLATEPERPGAAAQPAASPPPQQPPQPASASCEQPSADTAIKVEGGAAASDQILPEAEVRLGQ SGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIII PVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSI PVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVN LIGMMLLLCHWDGCLQFLVPMLQDFPHDCWVSINGMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMS DVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQ GKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGK KMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMR RAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVRHDREMAHCAHRVQAAASATPTPT PVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGNLGAGQTPRHPRRLQSLIPSALGSA SPASSPSQVDTPSSSSFHIQQLAGFSAPPGLSPLLPSSSSSPPPGACSSPPAPTPSTSTAATTTGFGHFH KALGGSLSSSDSPLLTPLQPGARSPQAAQPPPPLPGARGGLGLLEHFLPPPPSSRSPSSSPGQLGQPPGE LSPGLAAGPPSTPETPPRPERPSFMAGASGGASPVAFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHGSL LLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFSLYPRA GGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARAASSGGPPLTAAPQREPGARSEPV RSKLPSNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021658
ORF Size	3594 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021658.1, NP_067690.1
RefSeq Size	3971 bp
RefSeq ORF	3597 bp
Locus ID	59266
UniProt ID	Q9JKA7
MW	128.8 kDa
Gene Summary	may act as a neuronal pacemaker channel; may play a role in neurotransmitter release [RGD, Feb 2006]

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