Pi4ka (NM_022301) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pik4ca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN214160 representing NM_022301 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAGTAGATTTTCATGGAATCTTCCAGCTAGATGAAAGACGGAGAGATGCAGTGATTGCTCTGG GCATTTTTCTCATTGAGTCTGATCTTCAGCACAAAGATTGTATTGTTCCTTATCTTCTTCGACTTCTCAG GGGTCTTCCAAAAGTCTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCGGTTGCA GAAAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCGCTTAGGGATG AGATTTTAGAGGCACTTTTGCAGGTTTTACATGTCCTTCTGGGGATGTGCCAGGCCCTGGAGATTCAAGA GAAAGAATACCTTTGCAAGTATGCAATCCCATGCCTGATAGGGATCTCGAAATCTTTTGGACGTTACAGC AATTCAGAAGAATCTCTCCTCTCAAAGCTTTTTCCCAAAGTCCCTCCTCATTCCCTGAGAATCCCCGAAG AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTGCCCAGCAACCTGCTGACTGT CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT GGCATGCCCCCTCCCAGCTCCCCTGGAGGATCTGCTTTTCACTATTTTGAAGCCTCTTGCCTGCCTGATG GAACCACCTTGGAGCCCGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTGTCTCTCCCCTGTTCAA TGGTATCACCTATAAGGAGTTCTATATTCCACTGGAAATGCTTCGAGAACTCTTAAACTTGGTAAAGAAG ATTGTTGAGGAGCCTGTTCTCAAGTCCTTAGATGCAGCTGTAGCCAGAGTGATGGAGGCCAATCCCAGTG CTGACCTATACTACACGACCTTCAGTGATCCCGTCTACCTGACCATGTTCAAGATGCTTCGAGACACTCT GTACTATATGAAAGACTTACCCACCTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC ATGAGCCAAGGGGAACTCCAAAAGATTCTCCATGATGCAGACAGGATCCACAGTGAGATGAGCCCACTCA AACTGCGCTGCCAAGCAAATGCTGCCTGCGTAGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGCAAGGTCATCATCGCCCAT TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTATGTGAACGGTTCCCGGTGGTGGTCCACT CAGTGACACCATCTTTGAGAGACTTTCTGGTGATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATTAGTGTCACCAATGAGCATTCTGAGTCAACT CTGAATGTCTTGCCCGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGGGACATCGCCATTGACA ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT CTCCAACCGGCTCTATATCTCCCAGGAGAGCGATAAGGATGCTCACTTGATTCCTGACCATACCATTCGT GCCTTGGGACACATTGCAGTGGCTCTGAGGGATACCCCAAAGGTCATGGAGCCAATTCTGCAGCAGAAAT TTTGCCAGCCCCCCTCACCCCTCGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTTATCACTGGAAA TCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAACAAATCAGTGTGAAGGCCAGTTCAGTTGTATAT TCAGCCACCAAAGACTACAAAGACCATGGCTACAGGCACTGCTCCCTCGCAGTCATCAATGCCCTGGCCA ACATCGCAGCCAACATTCAGGAGGAGCACCTTGTGGATGAGCTGTTGATGAACCTGCTGGAACTGTTTGT GCAGCTGGGGCTGGAGGGGAAGAGAGCCAGTGAGAGGGCCAGCGAGAAGGGGCCCGCCCTGAAGGCTTCC AGCAGTGCAGGGAATTTGGGAGTGCTCATTCCTGTAATAGCCGTGCTCACCCGACGATTGCCACCCATCA AAGAAGCTAAACCTAGGCTACAAAAGCTTTTCCGAGACTTCTGGCTCTACTCTGTGCTAATGGGATTCGC TGTGGAAGGCTCAGGGCTCTGGCCGGAAGAGTGGTATGAAGGAGTCTGTGAAATAGCAACCAAGTCACCT CTGCTTACCTTTCCCAGCAAAGAGCCTCTTCGATCAGTCCTCCAGTACAACTCAGCCATGAAAAATGATA CAGTGACCCCGGCTGAGCTGAATGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCCTCCTGAGGT GTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCCACCTATCTCTTGTCTGTGTATCGGCTGGAATAC ATGAGGGTGCTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTCGAGGATAAAGCTA TTCAGAAAGACAAATCTGGGATGATGCAGTGTGTCATCGCTGTTGCTGACAAAGTATTTGATGCCTTCCT GAACATGATGGCAGAAAAAGCCAAGACCAAGGAGAATGAAGAGGAGTTGGAGCGGCATGCCCAGTTTCTG CTGGTGAACTTCAACCACATTCACAAGAGGATACGCAGAGTGGCTGACAAGTACCTGTCTGGTCTGGTGG ATAAGTTTCCACACTTGCTCTGGAGTGGGACTGTGCTGAAGACCATGTTGGACATCCTGCAGACCCTGTC ACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTATTATGACATTCCTGACGCCCCCTATCGGATC ACGGTTCCTGACACGTATGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCACGCTGTGGGATGATCC TGCAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTGCAGGAATATCTGAACAAGCATCA GAACTGGGTGTCAGGACTGTCCCAGCACACGGGATTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCC GGCTACAACAAGCAGAACACAACTCTTGGGGTAACTCAGCTCACTGAGCGCCCGGCCTGTGTGAAGAAAG ACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTGCACGGGATGATTCG GTTCTCAGGTGCCACAGGCCAGATGTCCGACCTGAACAAAATGATGGTCCAGGATCTGATTACAGCTCTG GACCACAGTCATCCTCAGCACTATACCCAAGCCATGTTCAAATTGACGGCCATGCTCATTAGCAGCAAAG ATTGTGACCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGGATGTTCAACGAGCATGGCATGGA GACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTCATGCGGGAG ATGGCAGGAGCCTGGCACATGACAGTGGAGCAGAAGTTCGGCCTCTTCTCTGCAGAAACAAAGGAAGCAG ACCCCCTGGCAGCGTCAGAAGCAAGTCAGCCACGACCCTGTCCCCCTGAAGTGACACCTCACTACATCTG GATTGATTTCCTGGTGCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAGATCTTCTCA AGCCTGCTGCAGCGCTCCATGTCCCTCCACATCGGTGGAGCCAGGGGGAGCATGAACAGGCATGTGGCAG CCATTGGACCACGTTTCAAACTGCTGACCCTGGGGCTGTCCCTCCTACACGCTGATGTGGTTCCAAATGC AACCATTCGAAACGTGCTTCGAGAGAAGATCTACTCAACAGCCTTTGACTATTTCAGTTGTCCACCAAAG TTCCCTACTCAAGGAGAAAAGAGGCTGCGTGAAGATATAAGCATCATGATTAAGTTTTGGACTGCCATGT TCTCAGATAAGAAGTACCTGACTGCCAGCCAACTCGTTCCCCCAGATAACCAGGACACCCGAAGCAACTT GGATATAACTGTGGGCTCTCGACAACAGGCTACACAGGGATGGATCAACACATACCCCCTCTCCAGTGGC ATGTCAACTATCTCCAAAAAATCAGGCATGTCTAAGAAAACCAACCGAGGTTCCCAGCTGCACAAGTACT ACATGAAGCGTAGGACGCTGCTGCTGTCACTGTTGGCCACTGAGATCGAGCGCCTCATCACTTGGTACAA CCCGCTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGTGAGAACAGTGTTGCCAACTGGAGGTCCAAG TATATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGACCATCTCTCCATACCTCG CTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCTCGTTCGCCT GGACCCAGGAGCTGTTAGTGATGTCCCTGAAGCTATCAAGTTCCTTGTCACCTGGCACACCATCGATGCT GATGCTCCAGAGCTTAGCCATGTGTTATGCTGGGCACCCACGGACCCGCCCACGGGCCTCTCCTACTTCT CCAGCATGTACCCCCCACACCCTCTCACTGCACAGTATGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGA TGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTATGTGCGCGAG TACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAATATGAAAACTAACA TCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATTGGTGACCTTCTGGAGCAGTTAGTAGAAGA AATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTTAACAAGATC ACCAACGTGTCAGCTATCATCAAGCCCTACCCTAAAGGCGATGAAAGAAAGAAGGCTTGTCTGTCAGCCC TGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATTGTGCTAGACATTGA CTACAAGTCAGGGACACCAATGCAGAGTGCTGCAAAAGCGCCGTATCTTGCCAAGTTTAAGGTGAAGCGA TGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACACAGAGGATGAGTGCCGCAGGC AAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTGCCGACAGGA CATGCTAGCCCTGCAGATCATCGACCTTTTCAAGAACATCTTCCAGCTGGTTGGCCTGGACCTCTTCGTT TTTCCCTATCGGGTAGTGGCTACTGCCCCTGGGTGTGGGGTGATTGAGTGCATTCCAGACTGTACCTCCC GAGACCAGCTAGGCCGCCAGACTGACTTCGGCATGTATGACTACTTTACACGCCAGTATGGGGACGAGTC TACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCGGCCTACAGCCTCCTGCTGTTC CTGCTGCAGATCAAGGACAGGCACAACGGCAACATCATGCTGGACAAGAAGGGACACATCATCCACATAG ACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAGCCAGACATCAAGCTGACAGA CGAGATGGTGATGATTATGGGGGGCAAGATGGAAGCCACACCCTTCAAGTGGTTCATGGAGATGTGCGTC CGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGTTGGACACAG GCCTGCCCTGTTTCCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACATGACAGAGCG GGAGGCTGCAAATTTTATCATGAAGATCATCCAAAACTGTTTCCTCAGCAACAGGAGCCGGACCTACGAC ATGATCCAGTACTACCAGAATGATATCCCCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022301
Insert Size	6126 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022301.1, NP_071637.1
RefSeq Size	6579 bp
RefSeq ORF	6126 bp
Locus ID	64161
UniProt ID	O08662
Gene Summary	catalyzes the phosphorylation of phosphatidylinositol (PI) at the D-4 position of inositol ring [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.