Pi4ka (NM_022301) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pik4ca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR214160 representing NM_022301 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAGTAGATTTTCATGGAATCTTCCAGCTAGATGAAAGACGGAGAGATGCAGTGATTGCTCTGG GCATTTTTCTCATTGAGTCTGATCTTCAGCACAAAGATTGTATTGTTCCTTATCTTCTTCGACTTCTCAG GGGTCTTCCAAAAGTCTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCGGTTGCA GAAAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCGCTTAGGGATG AGATTTTAGAGGCACTTTTGCAGGTTTTACATGTCCTTCTGGGGATGTGCCAGGCCCTGGAGATTCAAGA GAAAGAATACCTTTGCAAGTATGCAATCCCATGCCTGATAGGGATCTCGAAATCTTTTGGACGTTACAGC AATTCAGAAGAATCTCTCCTCTCAAAGCTTTTTCCCAAAGTCCCTCCTCATTCCCTGAGAATCCCCGAAG AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTCCGCTCCATCCTGCCCAGCAACCTGCTGACTGT CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT GGCATGCCCCCTCCCAGCTCCCCTGGAGGATCTGCTTTTCACTATTTTGAAGCCTCTTGCCTGCCTGATG GAACCACCTTGGAGCCCGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTGTCTCTCCCCTGTTCAA TGGTATCACCTATAAGGAGTTCTATATTCCACTGGAAATGCTTCGAGAACTCTTAAACTTGGTAAAGAAG ATTGTTGAGGAGCCTGTTCTCAAGTCCTTAGATGCAGCTGTAGCCAGAGTGATGGAGGCCAATCCCAGTG CTGACCTATACTACACGACCTTCAGTGATCCCGTCTACCTGACCATGTTCAAGATGCTTCGAGACACTCT GTACTATATGAAAGACTTACCCACCTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC ATGAGCCAAGGGGAACTCCAAAAGATTCTCCATGATGCAGACAGGATCCACAGTGAGATGAGCCCACTCA AACTGCGCTGCCAAGCAAATGCTGCCTGCGTAGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGCAAGGTCATCATCGCCCAT TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTATGTGAACGGTTCCCGGTGGTGGTCCACT CAGTGACACCATCTTTGAGAGACTTTCTGGTGATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATTAGTGTCACCAATGAGCATTCTGAGTCAACT CTGAATGTCTTGCCCGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGGGACATCGCCATTGACA ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT CTCCAACCGGCTCTATATCTCCCAGGAGAGCGATAAGGATGCTCACTTGATTCCTGACCATACCATTCGT GCCTTGGGACACATTGCAGTGGCTCTGAGGGATACCCCAAAGGTCATGGAGCCAATTCTGCAGCAGAAAT TTTGCCAGCCCCCCTCACCCCTCGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTTATCACTGGAAA TCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAACAAATCAGTGTGAAGGCCAGTTCAGTTGTATAT TCAGCCACCAAAGACTACAAAGACCATGGCTACAGGCACTGCTCCCTCGCAGTCATCAATGCCCTGGCCA ACATCGCAGCCAACATTCAGGAGGAGCACCTTGTGGATGAGCTGTTGATGAACCTGCTGGAACTGTTTGT GCAGCTGGGGCTGGAGGGGAAGAGAGCCAGTGAGAGGGCCAGCGAGAAGGGGCCCGCCCTGAAGGCTTCC AGCAGTGCAGGGAATTTGGGAGTGCTCATTCCTGTAATAGCCGTGCTCACCCGACGATTGCCACCCATCA AAGAAGCTAAACCTAGGCTACAAAAGCTTTTCCGAGACTTCTGGCTCTACTCTGTGCTAATGGGATTCGC TGTGGAAGGCTCAGGGCTCTGGCCGGAAGAGTGGTATGAAGGAGTCTGTGAAATAGCAACCAAGTCACCT CTGCTTACCTTTCCCAGCAAAGAGCCTCTTCGATCAGTCCTCCAGTACAACTCAGCCATGAAAAATGATA CAGTGACCCCGGCTGAGCTGAATGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCCTCCTGAGGT GTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCCACCTATCTCTTGTCTGTGTATCGGCTGGAATAC ATGAGGGTGCTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTCGAGGATAAAGCTA TTCAGAAAGACAAATCTGGGATGATGCAGTGTGTCATCGCTGTTGCTGACAAAGTATTTGATGCCTTCCT GAACATGATGGCAGAAAAAGCCAAGACCAAGGAGAATGAAGAGGAGTTGGAGCGGCATGCCCAGTTTCTG CTGGTGAACTTCAACCACATTCACAAGAGGATACGCAGAGTGGCTGACAAGTACCTGTCTGGTCTGGTGG ATAAGTTTCCACACTTGCTCTGGAGTGGGACTGTGCTGAAGACCATGTTGGACATCCTGCAGACCCTGTC ACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTATTATGACATTCCTGACGCCCCCTATCGGATC ACGGTTCCTGACACGTATGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCACGCTGTGGGATGATCC TGCAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTGCAGGAATATCTGAACAAGCATCA GAACTGGGTGTCAGGACTGTCCCAGCACACGGGATTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCC GGCTACAACAAGCAGAACACAACTCTTGGGGTAACTCAGCTCACTGAGCGCCCGGCCTGTGTGAAGAAAG ACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTGCACGGGATGATTCG GTTCTCAGGTGCCACAGGCCAGATGTCCGACCTGAACAAAATGATGGTCCAGGATCTGATTACAGCTCTG GACCACAGTCATCCTCAGCACTATACCCAAGCCATGTTCAAATTGACGGCCATGCTCATTAGCAGCAAAG ATTGTGACCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGGATGTTCAACGAGCATGGCATGGA GACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTCATGCGGGAG ATGGCAGGAGCCTGGCACATGACAGTGGAGCAGAAGTTCGGCCTCTTCTCTGCAGAAACAAAGGAAGCAG ACCCCCTGGCAGCGTCAGAAGCAAGTCAGCCACGACCCTGTCCCCCTGAAGTGACACCTCACTACATCTG GATTGATTTCCTGGTGCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAGATCTTCTCA AGCCTGCTGCAGCGCTCCATGTCCCTCCACATCGGTGGAGCCAGGGGGAGCATGAACAGGCATGTGGCAG CCATTGGACCACGTTTCAAACTGCTGACCCTGGGGCTGTCCCTCCTACACGCTGATGTGGTTCCAAATGC AACCATTCGAAACGTGCTTCGAGAGAAGATCTACTCAACAGCCTTTGACTATTTCAGTTGTCCACCAAAG TTCCCTACTCAAGGAGAAAAGAGGCTGCGTGAAGATATAAGCATCATGATTAAGTTTTGGACTGCCATGT TCTCAGATAAGAAGTACCTGACTGCCAGCCAACTCGTTCCCCCAGATAACCAGGACACCCGAAGCAACTT GGATATAACTGTGGGCTCTCGACAACAGGCTACACAGGGATGGATCAACACATACCCCCTCTCCAGTGGC ATGTCAACTATCTCCAAAAAATCAGGCATGTCTAAGAAAACCAACCGAGGTTCCCAGCTGCACAAGTACT ACATGAAGCGTAGGACGCTGCTGCTGTCACTGTTGGCCACTGAGATCGAGCGCCTCATCACTTGGTACAA CCCGCTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGTGAGAACAGTGTTGCCAACTGGAGGTCCAAG TATATCAGCCTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGACCATCTCTCCATACCTCG CTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCTCGTTCGCCT GGACCCAGGAGCTGTTAGTGATGTCCCTGAAGCTATCAAGTTCCTTGTCACCTGGCACACCATCGATGCT GATGCTCCAGAGCTTAGCCATGTGTTATGCTGGGCACCCACGGACCCGCCCACGGGCCTCTCCTACTTCT CCAGCATGTACCCCCCACACCCTCTCACTGCACAGTATGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGA TGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTATGTGCGCGAG TACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAATATGAAAACTAACA TCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATTGGTGACCTTCTGGAGCAGTTAGTAGAAGA AATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTTAACAAGATC ACCAACGTGTCAGCTATCATCAAGCCCTACCCTAAAGGCGATGAAAGAAAGAAGGCTTGTCTGTCAGCCC TGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATTGTGCTAGACATTGA CTACAAGTCAGGGACACCAATGCAGAGTGCTGCAAAAGCGCCGTATCTTGCCAAGTTTAAGGTGAAGCGA TGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACACAGAGGATGAGTGCCGCAGGC AAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTGCCGACAGGA CATGCTAGCCCTGCAGATCATCGACCTTTTCAAGAACATCTTCCAGCTGGTTGGCCTGGACCTCTTCGTT TTTCCCTATCGGGTAGTGGCTACTGCCCCTGGGTGTGGGGTGATTGAGTGCATTCCAGACTGTACCTCCC GAGACCAGCTAGGCCGCCAGACTGACTTCGGCATGTATGACTACTTTACACGCCAGTATGGGGACGAGTC TACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCGGCCTACAGCCTCCTGCTGTTC CTGCTGCAGATCAAGGACAGGCACAACGGCAACATCATGCTGGACAAGAAGGGACACATCATCCACATAG ACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAGCCAGACATCAAGCTGACAGA CGAGATGGTGATGATTATGGGGGGCAAGATGGAAGCCACACCCTTCAAGTGGTTCATGGAGATGTGCGTC CGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGTTGGACACAG GCCTGCCCTGTTTCCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACATGACAGAGCG GGAGGCTGCAAATTTTATCATGAAGATCATCCAAAACTGTTTCCTCAGCAACAGGAGCCGGACCTACGAC ATGATCCAGTACTACCAGAATGATATCCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR214160 representing NM_022301 Red=Cloning site Green=Tags(s) MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCIVPYLLRLLRGLPKVYWVEESTARKGRGNLPVA ESFSFCLVTLLSDVACRDPSLRDEILEALLQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISKSFGRYS NSEESLLSKLFPKVPPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPER GMPPPSSPGGSAFHYFEASCLPDGTTLEPEYYFSTISSSFSVSPLFNGITYKEFYIPLEMLRELLNLVKK IVEEPVLKSLDAAVARVMEANPSADLYYTTFSDPVYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFN MSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSEST LNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIR ALGHIAVALRDTPKVMEPILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVY SATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKAS SSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSP LLTFPSKEPLRSVLQYNSAMKNDTVTPAELNELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEY MRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHAQFL LVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRI TVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFA GYNKQNTTLGVTQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLITAL DHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPFMRE MAGAWHMTVEQKFGLFSAETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFS SLLQRSMSLHIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPK FPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSG MSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSK YISLSEKQWKDNVNLAWTISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDA DAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVRE YILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFFNKI TNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKR CGVSELEKEGLQCRSDTEDECRRQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFV FPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLF LLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCV RGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKIIQNCFLSNRSRTYD MIQYYQNDIPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_022301
ORF Size	6123 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022301.1, NP_071637.1
RefSeq Size	6579 bp
RefSeq ORF	6126 bp
Locus ID	64161
UniProt ID	O08662
MW	231.3 kDa
Gene Summary	catalyzes the phosphorylation of phosphatidylinositol (PI) at the D-4 position of inositol ring [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.