Uba1 (NM_001014080) Rat Untagged Clone

CAT＃: RN211933

Uba1 (untagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)

Datasheet
SDS

  "NM_001014080" in other vectors (3)

CNY 17,010.00

货期*
7周
规格
    • 10 ug
Cited in 3 publications.

Product images

经常一起买 (3)
TF81001
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Ube1x
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211933 representing NM_001014080
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCAGGTTCTAACTGCT
CCTCTGCACAGTCAGTGCTGTCCGAAGTGTCTTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA
AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC
CAGACATCCAGTGTCCTCGTCTCAGGCTTGCGGGGCTTAGGTGTCGAGATTGCTAAGAACATCATCCTTG
GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGCACTACACAGTGGGCTGATCTCTCCTCCCAGTTTTA
CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCTGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC
TATGTACCTGTTACTGCCTACACTGGGCCTCTTGTTGAAGACTTCCTTAGTGGTTTCCAGGTGGTGGTCC
TTACCAACAGCCCCCTGGAAGAACAGCTTCGAGTGGGTGAGTTCTGTCATAGCCGTGGCATCAAGCTAGT
GGTGGCAGATACAAGAGGCCTATTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTTCTCACAGAT
TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT
GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGGT
CCAACTCAATGGATGTCAGCCCATAGAGATCAAAGTGCTGGGTCCTTATACTTTTAGTATCTGTGACACC
TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGCCAGGTCAAAGTACCTAAAAAGATTAGCTTTA
AATCCTTGCCAGCATCACTGGCAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC
CCAACTGCACATTGGCTTTCAAGCTCTGCACCAATTCTGTGCTCAGCACAACCGACCACCTCGACCACGA
AATGAGGAAGATGCAACAGAACTGGTGACCCTAGCTCAGGCTGTGAATGCTCGGTCCCCACCTGCAGTAC
AACAGGACAACGTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCTAT
AAATGCTTTCATTGGGGGCCTCGCTGCCCAGGAAGTCATGAAGGCCTGTTCTGGAAAGTTTATGCCCATC
ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGACAAGT
GCCTCCCACGTCAGAACCGTTATGATGGGCAGGTAGCTGTATTTGGCTCAGACCTTCAAGAGAAGCTGGG
CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT
GGGTTAGGCTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCGAATCTGA
ACCGACAGTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG
ACAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGCCCTGACACGGAGCGCATCTAT
GATGATGATTTTTTCCAAAATTTGGATGGTGTGGCCAATGCACTGGACAATGTAGATGCCCGGATGTACA
TGGATCGCCGCTGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAATGT
GCAGGTGGTGATCCCCTTCCTGACAGAATCATACAGCTCTAGCCAGGACCCACCAGAGAAATCCATCCCC
ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTACAGTGGGCCCGGGATGAATTTGAAG
GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT
GCGGCTGGCGGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACTTGGGGCGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATACGGC
AGCTGCTGCACAACTTTCCTCCCGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG
ATGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC
CTTTTTGCCCAGACCTATGGGTTGACTGGCTCCCAAGACCGAGCTGCTGTGGCCTCACTCCTACAGTCAG
TACAAGTTCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATCCATGTTTCTGACCAGGAGTTGCAGAGCGC
CAATGCCTCTGTTGATGACAGCCGTCTCGAGGAGCTCAAAGCCACACTGCCGAGCCCAGACAAGTTACCT
GGATTTAAGATGTACCCCATTGATTTTGAAAAGGACGATGACAGCAATTTCCACATGGATTTCATTGTGG
CTGCATCTAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGGCACAAGAGCAAGCTGATCGC
AGGGAAGATCATCCCAGCTATCGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAACTCTAC
AAGGTAGTTCAGGGGCACCAACAACTTGATTCCTACAAAAATGGTTTCCTGAATTTGGCCCTGCCCTTCT
TTGGGTTTTCGGAACCTCTTGCTGCGCCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG
CTTTGAAGTACAAGGGCTACAGCCTAATGGTGAGGAGATGACTCTCAAGCAGTTCCTTGATTACTTTAAG
ACGGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGTGTGTCCATGCTCTATTCTTTCTTCATGCCAG
CTGCTAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT
GGGCCGCCATGTGCGTGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGTGGCGAGGACGTCGAGGTC
CCCTATGTACGATATACAATTCGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001014080
Insert Size 3177 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001014080.1, NP_001014102.1
RefSeq Size 3618 bp
RefSeq ORF 3177 bp
Locus ID 314432
UniProt ID Q5U300
Gene Summary Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (3)

The use of this cDNA Clones has been cited in the following citations:
Central catalytic domain of BRAP (RNF52) recognizes the types of ubiquitin chains and utilizes oligo-ubiquitin for ubiquitylation ,null, Biochemical Journal ,PubMed ID 28768733 [Uba1]
Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis ,null, Proceedings of the National Academy of Sciences of the United States of America ,PubMed ID 26976599 [Uba1]
Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage. ,null, Cell cycle (Georgetown, Tex.) ,PubMed ID 22456334 [Uba1]

Other Versions

SKU Description Size Price
RR211933 Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
    • 10 ug

CNY 17,010.00

RR211933L3 Lenti ORF clone of Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
    • 10 ug

CNY 18,620.00

RR211933L4 Lenti ORF clone of Uba1 (mGFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
    • 10 ug

CNY 20,330.00

其它Uba1产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

    • 5 x 200 ul

CNY 1,280.00 暂停销售

RR211933L4V

Lenti ORF particles, Uba1 (GFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), 200ul, >10^7 TU/mL

    • 200 ul

CNY 20,900.00 暂停销售

RR211933L3

Lenti ORF clone of Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)

    • 10 ug

CNY 18,620.00

RR211933

Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)

    • 10 ug

CNY 17,010.00

RR211933L4

Lenti ORF clone of Uba1 (mGFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)

    • 10 ug

CNY 20,330.00

RR211933L3V

Lenti ORF particles, Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), 200ul, >10^7 TU/mL

    • 200 ul

CNY 18,896.00 暂停销售

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