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Uba1 (NM_001014080) Rat Untagged Clone
CAT＃: RN211933
Uba1 (untagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug)
CNY 17,010.00
货期*
7周
规格
| Cited in 3 publications. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ube1x |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN211933 representing NM_001014080
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCAGGTTCTAACTGCT CCTCTGCACAGTCAGTGCTGTCCGAAGTGTCTTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC CAGACATCCAGTGTCCTCGTCTCAGGCTTGCGGGGCTTAGGTGTCGAGATTGCTAAGAACATCATCCTTG GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGCACTACACAGTGGGCTGATCTCTCCTCCCAGTTTTA CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCTGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC TATGTACCTGTTACTGCCTACACTGGGCCTCTTGTTGAAGACTTCCTTAGTGGTTTCCAGGTGGTGGTCC TTACCAACAGCCCCCTGGAAGAACAGCTTCGAGTGGGTGAGTTCTGTCATAGCCGTGGCATCAAGCTAGT GGTGGCAGATACAAGAGGCCTATTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTTCTCACAGAT TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGGT CCAACTCAATGGATGTCAGCCCATAGAGATCAAAGTGCTGGGTCCTTATACTTTTAGTATCTGTGACACC TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGCCAGGTCAAAGTACCTAAAAAGATTAGCTTTA AATCCTTGCCAGCATCACTGGCAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC CCAACTGCACATTGGCTTTCAAGCTCTGCACCAATTCTGTGCTCAGCACAACCGACCACCTCGACCACGA AATGAGGAAGATGCAACAGAACTGGTGACCCTAGCTCAGGCTGTGAATGCTCGGTCCCCACCTGCAGTAC AACAGGACAACGTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCTAT AAATGCTTTCATTGGGGGCCTCGCTGCCCAGGAAGTCATGAAGGCCTGTTCTGGAAAGTTTATGCCCATC ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGACAAGT GCCTCCCACGTCAGAACCGTTATGATGGGCAGGTAGCTGTATTTGGCTCAGACCTTCAAGAGAAGCTGGG CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT GGGTTAGGCTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCGAATCTGA ACCGACAGTTTCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG ACAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGCCCTGACACGGAGCGCATCTAT GATGATGATTTTTTCCAAAATTTGGATGGTGTGGCCAATGCACTGGACAATGTAGATGCCCGGATGTACA TGGATCGCCGCTGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAATGT GCAGGTGGTGATCCCCTTCCTGACAGAATCATACAGCTCTAGCCAGGACCCACCAGAGAAATCCATCCCC ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTACAGTGGGCCCGGGATGAATTTGAAG GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT GCGGCTGGCGGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA CAGACTTGGGGCGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATACGGC AGCTGCTGCACAACTTTCCTCCCGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG ATGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC CTTTTTGCCCAGACCTATGGGTTGACTGGCTCCCAAGACCGAGCTGCTGTGGCCTCACTCCTACAGTCAG TACAAGTTCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATCCATGTTTCTGACCAGGAGTTGCAGAGCGC CAATGCCTCTGTTGATGACAGCCGTCTCGAGGAGCTCAAAGCCACACTGCCGAGCCCAGACAAGTTACCT GGATTTAAGATGTACCCCATTGATTTTGAAAAGGACGATGACAGCAATTTCCACATGGATTTCATTGTGG CTGCATCTAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGGCACAAGAGCAAGCTGATCGC AGGGAAGATCATCCCAGCTATCGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAACTCTAC AAGGTAGTTCAGGGGCACCAACAACTTGATTCCTACAAAAATGGTTTCCTGAATTTGGCCCTGCCCTTCT TTGGGTTTTCGGAACCTCTTGCTGCGCCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG CTTTGAAGTACAAGGGCTACAGCCTAATGGTGAGGAGATGACTCTCAAGCAGTTCCTTGATTACTTTAAG ACGGAGCACAAATTAGAGATCACCATGCTGTCCCAGGGTGTGTCCATGCTCTATTCTTTCTTCATGCCAG CTGCTAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT GGGCCGCCATGTGCGTGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGTGGCGAGGACGTCGAGGTC CCCTATGTACGATATACAATTCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001014080 |
| Insert Size | 3177 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001014080.1, NP_001014102.1 |
| RefSeq Size | 3618 bp |
| RefSeq ORF | 3177 bp |
| Locus ID | 314432 |
| UniProt ID | Q5U300 |
| Gene Summary | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.[UniProtKB/Swiss-Prot Function] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Central catalytic domain of BRAP (RNF52) recognizes the types of ubiquitin chains and utilizes oligo-ubiquitin for ubiquitylation
,null,
Biochemical Journal
,PubMed ID 28768733
[Uba1]
|
|
Stability of the human polymerase δ holoenzyme and its implications in lagging strand DNA synthesis
,null,
Proceedings of the National Academy of Sciences of the United States of America
,PubMed ID 26976599
[Uba1]
|
|
Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage.
,null,
Cell cycle (Georgetown, Tex.)
,PubMed ID 22456334
[Uba1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR211933 | Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 17,010.00 |
|
| RR211933L3 | Lenti ORF clone of Uba1 (Myc-DDK-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 18,620.00 |
|
| RR211933L4 | Lenti ORF clone of Uba1 (mGFP-tagged ORF) - Rat ubiquitin-like modifier activating enzyme 1 (Uba1), (10 ug) |
CNY 20,330.00 |
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