Sema5a (NM_001107659) Rat Untagged Clone

CAT＃: RN208579

Sema5a (untagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)

Datasheet
SDS

  "NM_001107659" in other vectors (3)

CNY 17,200.00

货期*
7周
规格
    • 10 ug

Product images

经常一起买 (3)
TF81001
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN208579 representing NM_001107659
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTCGGCCTGAGA
CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCCGTCGTCTCCTACAAAGAAATTGGCCCCTGGTT
ACGGGAATTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT
GTCGTAGGAGCAAGAAACTATCTCTTCAGACTACAGCTCGAGGATCTGTCTCTCATCCAGGCTGTGCAGT
GGGAGTGTGATGAAGCCACGAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA
CATACGAGTGCTCTTAGTGGGCGGGGACCGGCTCTTCACCTGTGGGACCAATGCCTTCACACCCGTCTGT
ACCATCCGCTCCTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCTCGATGTCCCTATA
GTCCCCAGCACAATTCTACAGCTCTACTCACTGCCAGTGGCGAGCTCTATGCTGCAACAGCCATGGATTT
CCCAGGGCGCGATCCAGCCATTTATCGAAGTCTGGGCACTTTACCTCCTCTTCGAACTGCACAGTACAAC
TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGGAACTTCACCTACTTCTTCTTCC
GAGAAAATGCCGTGGAACACGACTGTGGGAAGACTGTGTTCTCCAGGGCAGCCAGGGTCTGCAAGAATGA
CATTGGAGGACGATTTCTTCTGGAGGATACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCTCGG
CCTGGTGAGGTGCCGTTCTACTACAACGAGCTTCAAAGCACCTTCTTCCTGCCGGAGCTGGATCTGATCT
ACGGCATCTTCACCACCAATGTAAACAGCATTGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT
CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAGAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC
CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTATGTAAACCTGACGGAAAGAAACCTAC
AGGATGCTCAGAAGTTCATCCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCTTCCTTCATGGA
AGACAACAGCCGTTTCTCCCATGTGGCTGTCGATGTTGTGCAAGGCAGGGACACCCTTGTCCATATCATC
TATCTGGCCACAGATTATGGCACCATTAAAAAAGTGCGGGCTCCGCTGAGTCAGAGCTCGGGCAGCTGTT
TGCTAGAAGAGATTGAGCTTTTTCCAGAGAGGAAGAGTGAGCCCATCAGGAGCCTAAAGATCCTCCACAG
CCAGAGTGTCCTATTTGTGGGGCTACAGGAGCATGTGGTCAAGATCCCCCTGAAGAGATGCCATTTTCAT
CAAACACGTGGTGCCTGCATTGGTGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAGTGCA
CCAGCCTGGAGGAGAGCCTGAGCATGACCCAGTGGGATCAGAGCGTCCCCACCTGTCCGACCAGAAATCT
CACCGTAGATGGGAGCTTTGGCCCGTGGTCTCCATGGACACCCTGTACTCACACTGATGGCACTGCTGTG
GGATCCTGCCTCTGCCGGTCCCGATCCTGTGACAGCCCAGCTCCACAGTGTGGCGGCTGGCAGTGTGAGG
GCCCTAGAATGGAGATCACCAACTGTTCCAGGAATGGAGGCTGGACTCCCTGGACCTCCTGGTCTCCCTG
CAGCACCACTTGTGGCATTGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCAACCCTACGCCCAGGCATGGA
GGGCGTGTGTGCGTGGGCCAGAACAGGGAGGAAAGATACTGCAATGAACATTTACTCTGCCCACCACACG
TGTTCTGGACAGGCTGGGGACCTTGGGAACGCTGCACAGCCCAGTGCGGGGGTGGCATTCAAGCTCGCCG
TAGGACCTGTGAAAATGGGCCTGACTGTGCAGGATGCAATGTGGAATACCAGCCTTGTAACACCAATGCA
TGCCCAGAGTTGAAGAAGACCACACCATGGACCCCCTGGACACCTGTCAACATCTCTGACAATGGGGGCC
ACTATGAGCAGCGGTTCCGCTACACCTGTAAAGCTCGCCTGCCAGATCCAAACTTATTGGAAGTAGGAAG
ACAAAGGATAGAAATGCGGTACTGTTCTAGTGATGGGACCAGTGGCTGCTCCACAGATGGACTTTCTGGA
GACTTTCTAAGAGCTGGGAGATACTCTGCCCATACAGTCAATGGGGCATGGTCAGCCTGGACTTCCTGGT
CACAGTGCAGCAGAGACTGCAGCAGGGGCATTCGAAACAGGAAGCGTGTTTGCAACAACCCAGAGCCCAA
GTATGGAGGTATGCCATGCCTTGGTCCATCACTGGAGTTCCAGGAATGCAACATTTTACCCTGTCCAGTG
GATGGTGTGTGGTCTTGCTGGTCATCCTGGTCTAAATGCTCAGCAACTTGTGGGGGTGGACACTACATGA
GAACCCGTTCGTGTACGAATCCAGCCCCAGCATACGGAGGGGACATCTGCCTGGGACTGCACACGGAAGA
GGCCCTCTGTAACACACAGACCTGCCCAGAGAACTGGTCAGAGTGGTCAGAGTGGTCTGTGTGTGATGCA
TCTGGTACCCAGGTCCGTACTCGGCAGTGCATCCTTCTGTTTCCTGTGGGCAGCCAGTGTTCTGGAAATA
CTACAGAGAGCCGGCCTTGTGTATTTGACTCTAATTTCATCCCAGAAGTGTCTGTGGCAAGATCCAGCAG
CGTAGAAGAGAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATGGCTGTGGGACTTAGCAGTTCCATC
CTCGGCTGCCTCCTTACACTGCTTGTCTACACCTACTGCCAGCGGTACCAGCAGCAGTCCCATGATGCAA
CTGTCATCCATCCTGTTTCTCCCGCCGCCCTCAACAGCAGCATAACTAACCACATCAACAAACTGGATAA
ATACGATTCCGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAGAGAAACAAATAC
TTCAACCCACATCTCACTGGGAAGACCTACTCCAACGCCTACTTTACAGATCTCAACAATTATGATGAAT
ACTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107659
Insert Size 3225 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107659.2, NP_001101129.1
RefSeq Size 10501 bp
RefSeq ORF 3225 bp
Locus ID 310207
UniProt ID D3ZTD8
Gene Summary Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RR208579 Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)
    • 10 ug

CNY 17,200.00

RR208579L3 Lenti ORF clone of Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)
    • 10 ug

CNY 18,810.00

RR208579L4 Lenti ORF clone of Sema5a (mGFP-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)
    • 10 ug

CNY 20,620.00

其它Sema5a产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

    • 5 x 200 ul

CNY 1,280.00 暂停销售

RR208579L4

Lenti ORF clone of Sema5a (mGFP-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)

    • 10 ug

CNY 20,620.00

RR208579L3

Lenti ORF clone of Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)

    • 10 ug

CNY 18,810.00

RR208579L4V

Lenti ORF particles, Sema5a (GFP-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), 200ul, >10^7 TU/mL

    • 200 ul

CNY 21,185.00 暂停销售

RR208579L3V

Lenti ORF particles, Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), 200ul, >10^7 TU/mL

    • 200 ul

CNY 19,124.00 暂停销售

RR208579

Sema5a (Myc-DDK-tagged ORF) - Rat sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (Sema5a), (10 ug)

    • 10 ug

CNY 17,200.00

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