Vars (NM_053292) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Bat6; G7a; TrsVal; Vars2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208326 representing NM_053292 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATCCTCTACGTCTCTCCCCACCCCGACGCCTTCCCCAGCCTGCGAGCCCTCATCGCTGCCCGCT ACGGGGAAGCTGGGGACGGTCCCGGGTGGGGAGGCCCTCACCCTCGCATCTGCCTGCAGCCTCCCCCGAG CAGCCGGACTCCATTCCCCCCGCCTCGCCTGCCCGCCCTGGAGCAGGGGCCTGGCGGCCTGTGGGTGTGG GGGGCCCCGGCTGTAGCTCAGCTGCTGTGGCCAGCAGGCCTGGGAGGACCCGGGGGCAGCCGGGCAGCTG TCCTGGTCCAGCAGTGGGTCAGTTATGCCGACACGGAGCTCACACCAGCTGCCTGCGGGGCGACGCTGCC TGCTCTAGGACTTCGAGGTCCTGGTCAGGATCCCCAGGCTGCCCTTGGGGCCCTGGGCAAGGCCTTGAAC CCCTTGGAGGAATGGCTTCGTCTGCACACCTACCTGGCTGGGGACGCTCCCACTCTGGCTGACTTAGCTG CTGTGACAGCCTTACTGCTGCCCTTCCGATACGTTCTGGACCCTTCTGCCCGCCGGATCTGGGGTAATGT GACTCGCTGGTTTAACACCTGTGTCCGGCAACCAGAATTCCGGGCTGTGCTGGGAGAAGTGGTCCTGTAC TCAGGGGCCAGGTCTGTCACTCAGCAGCCAGGTTCCGAGATCACTGCACCCCAAAAGACAGCTGCTCAGC TCAAGAAAGAAGCAAAGAAACGGGAGAAACTAGAGAAATTCCAACAGAAGCAGAAGACGCAGCAGCAGCA GCCGGCACACGGGGAGAAGAAACCAAAACCAGAGAAGAAGGAGAAGCGAGACCCCGGGGTCATTACCTAT GACCTCCCTACCCCACCTGGGGAGAAGAAAGACGTAAGTGGCACCATGCCCGACTCCTACAGCCCTCAGT ATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCGGCAGGGCTTCTTCAAGCCAGAGTACGGGCGTCCTAG CGTGTCAGCACCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAACGTGACAGGTTCCCTG CACCTGGGCCACGCGCTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGGGGGGAGA CCACCCTATGGAACCCAGGCTGTGACCATGCTGGCATCGCCACCCAGGTGGTGGTGGAAAAGAAACTCTG GAAAGAGCGGGGTCTGAACCGTCACCAGCTGGGCCGCGAGGCCTTTCTTCAGGAGGTCTGGAAGTGGAAA GCGGAGAAGGGTGACAGGATTTACCACCAATTAAAGAAACTTGGCAGCTCCTTGGACTGGGATCGAGCCT GCTTCACCATGGATCCAAAATTGTCAGCAACTGTGACAGAAGCTTTTGTTAGGCTCCATGAAGAAGGGGT CATTTACCGCAGTACCCGCCTGGTCAACTGGTCCTGCACCCTCAACTCTGCCATTTCTGACATCGAGGTG GATAAGAAAGAGCTGACGGGCCGCACCCTGCTCTCTGTGCCTGGCTACAAGGAGAAGGTGGAGTTTGGGG TCCTCGTGTCCTTTGCCTACAAGGTCCAAGGCTCAGACAGCGACGAGGAGGTGGTGGTGGCAACAACCCG GATCGAGACAATGCTGGGGGACGTGGCTGTGGCTGTGCACCCCAAAGATCCCAGATACCAGCATTTAAAG GGAAAGAGTGTCGTCCACCCGTTCTTGTCCCGGAGCCTTCCCATTGTCTTTGATGACTTTGTGGACATGG AGTTTGGCACAGGTGCTGTGAAGATCACCCCGGCCCATGACCAGAATGACTATGAGGTTGGGCAGCGACA TAGGCTGGAGGCCATTAGCATCATGGACTCGAAGGGGGCCCTTGTCAATGTGCCTCCACCTTTCCTGGGC CTGCCCAGGTTTGAGGCCAGGAAGGCTGTGCTGGCGGCCCTGAAGGAGCAGGGCCTGTTCCGCGGCATCA AGGACAACCCCATGGTAGTGCCACTATGCAACCGCTCCAAGGATGTGGTGGAGCCTCTGCTAAGGCCACA GTGGTACGTGCGCTGTGGGGAGATGGCTCAGGCCGCCAGTGCTGCCGTGACCCGGGGAGACCTCCGTATA CTGCCTGAGGCCCATCAGCGGACGTGGCATTCTTGGATGGACAACATCCGAGACTGGTGCATCTCTCGGC AGCTGTGGTGGGGCCACCGAATCCCTGCCTACTTCATCACTGTCCATGACCCAGCGGTACCCCCTGGGGA GGACCCTGATGGGCGGTACTGGGTAAGCGGACGCACTGAAGCAGAGGCCCGGGAGAAGGCAGCACGGGAA TTTGGAGTGTCCCCTGACAAGATCAGCCTTCAGCAAGATGAGGATGTGTTAGACACCTGGTTCTCGTCTG GTCTCTTTCCCTTCTCCATCTTTGGCTGGCCCAATCAGTCAGAAGATCTCAGTGTGTTCTACCCTGGGAC CCTGCTGGAGACGGGCCATGATATCCTCTTCTTCTGGGTGGCGCGAATGGTCATGCTTGGCCTGAAGCTC ACTGAGAAGCTGCCCTTCAGAGAGGTCTACCTCCATGCAATCGTGCGGGACGCTCATGGCCGGAAGATGA GCAAGTCTCTCGGCAATGTCATCGACCCCCTGGATGTCATCCATGGAGTTTCCTTGCAGGGCCTCCATGA CCAACTACTGAACAGCAACCTGGATCCCAGTGAGGTGGAGAAAGCCAAAGAAGGACAGAGGGCCGACTTT CCAGCAGGGATTCCCGAGTGTGGCACTGATGCCCTACGCTTTGGACTCTGTGCCTACACATCCCAAGGTC GAGACATTAACCTGGATGTGAACAGGATCCTGGGGTACCGTCACTTCTGCAACAAACTCTGGAATGCCAC CAAGTTTGCTTTGCGCGGCCTTGGGAAGGGCTTTGTACCCTCACCAACCTCCAAGCCGGAAGGGCACGAG AGCCTGGTGGACCGCTGGATCCGCAGCCGGCTGGCGGAGGCCGTGAGGCTCAGCAATGAAGGCTTCCAGG CTTATGATTTCCCAGCCGTCACCACTGCCCAGTACAGCTTTTGGCTCTATGAGCTCTGTGATGTCTACTT GGAGTGCCTAAAACCCGTTCTGAACGGAGTGGACCAGGTAGCAGCAGACTGTGCTCGGCAAACCCTCTAC ACCTGCCTGGATGTCGGCCTGCGGCTGCTCTCACCCTTCATGCCCTTTGTCACAGAGGAGCTATTCCAGA GGCTGCCTCGGCGGACACCAAACGCTCCTGCTAGCCTCTGTGTCACCCCCTACCCAGAGCCCTCAGAGTG CTCCTGGAAGGACCCTGAAGCCGAAGCTGCTCTTGAGCTAGCGCTGAGCATCACTAGAGCTGTGCGCTCC CTGCGTGCAGACTACAACCTGACCCGGACCAGGCCCGACTGTTTCTTGGAAGTAGCTGATGAGGCTACGG GTGCCTTGGCCTCGGCAGTGTCGGCCTACGTGCAGACTCTGGCCAGTGCGGGTGTGGTGGCCGTCCTGGC CCTGGGTGCTCCCGCACCACAGGGCTGCGCTGTGGCTGTGGCCTCAGACCGCTGCTCCATCCACCTGCAG CTGCAGGGGCTCGTGGACCCAGCCCGGGAGCTGGGCAAGCTGCAGGCCAAGCGGAGTGAGGCACAGCGAC AGGCTCAGCGGCTGCAGGAGCGCCGTGCTGCCTCTGGCTACTCAGCAAAGGTGCCCCTTGAGGTCCAGGA GGCCGACGAAGTGAAGTTACAACAGACAGAGGCGGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTGTTT CAGAAGATGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053292
Insert Size	3795 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053292.1, NP_445744.1
RefSeq Size	3980 bp
RefSeq ORF	3795 bp
Locus ID	25009
UniProt ID	Q04462
Gene Summary	catalyze the aminoacylation of tRNA by valine [RGD, Feb 2006]

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